Antioxidant and Anti-Hyaluronidase Activities of Dragon Fruit Peel Extract and Kaempferol-3-O-Rutinoside

Abstract

Aging is a natural process in human life and is triggered by the presence of free radicals (ROS). The use of antioxidants from natural ingredients is one of the breakthroughs to overcome aging and counteract the harmful effects caused by the free radicals. This study aimed to determine and compare the antioxidant activity of H2O2 scavenging and hyaluronidase inhibition of red dragon fruit peel extract (DFPE) and kaempferol-3-o-rutinoside (KOR) compounds. Dragon fruit peel extract (DFPE) is obtained through extraction by maceration method using 70% ethanol solvent. The design of this study included antioxidant and anti-aging activity assay of EKBN and KOR at the series concentration of 15.63; 31.25; 62.50; 125; 250; 500 µg/mL through H2O2 scavenging, as well as the DFPE and KOR hyaluronidase inhibition assay at the series concentration of 5.21; 10.42; 20.83; 41.7; 83.33; 166.67 µg/mL. EKBN shows that the average activity of H2O2 scavenging is lower than KOR. In addition, the IC50 values of KOR for H2O2 scavenging is lower (351.46±2.30ug/mL) than DFPE (409.64±23.17ug/mL). While, KOR also has higher values of inhibitory activity than of the DFPE. However, the IC50 value of KOR for hyaluronidase inhibition activity was 84.07±10.46µg/mL, equivalent to the IC50 value of DFPE (85.32±10.24µg/mL). The presence of antioxidant and anti-aging activity in the EKBN is probably caused by betalain and the KOR compound itself contained in red dragon fruit. The results of the paired-samples T-test on antioxidant activity and anti-aging of DFPE and KOR showed non-significant difference. Thus, DFPE has an equivalent antioxidant and anti-aging through H2O2 scavenging and hyaluronidase activity as possessed by the KOR compound.

Antioxidant and Elastase Inhibitor Potential of Petals and Receptacle of Rose Flower (Rosa damascena)

“…The both extract RPE and RRE were later diluted in dimethyl sulfoxide (DMSO) with following concentrations: 5000, 2500, 1250, 625, 312.50, 156.25, 1000, 500, 250, 125, 62.50, and 31.25 µg/mL. This dilution are later called working concentrations (Mahadi et al, 2019;Vrianty et al, 2019;Siregar et al, 2019;Girsang et al, 2019;Liana et al, 2019;Asan et al, 2019;Prahastuti et al, 2020).…”

“…Some examples of plants that have antioxidant and antiaging effects are roselle roses (Hisbiscus sabdarifa) (Widowati et al, 2017), jasmine (Jasminum sambac) (Widowati et al, 2018b), ripe sesoot fruit (Garcinia picrorrhiza Miq.) (Utami et al, 2018), black soybeans (Glycine max [L.] Merr) (Asan et al, 2019), dragon fruit peel (Hylocereus polyrhizus) (Liana et al, 2019), pineapple (Vrianty et al, 2019), and zalacca skin (Salacca zalacca (Gaert.) Voss) (Girsang et al, 2019).…”

“…Voss) (Girsang et al, 2019). Sources of antioxidants are mangosteen peel (Garcinia mangostana L.) (Widowati et al, 2014), black tea, green tea, oolong tea (Camelia sinensis L.) (Widowati et al, 2015), green meniran (Phyllanthus niruri) (Rusmana et al, 2017), black soybean (Glycine max L. Merr) (Asan et al, 2019), dragon fruit peel (Hylocereus polyrhizus) (Liana et al, 2019), pineapple (Vrianty et al, 2019), and zalacca bark (Salacca zalacca (Gaert.) Voss) (Girsang et al, 2019).…”

Potentials of rose (Rosa damascena) petals and receptacles extract as antioxidant and antihyaluronidase

“…RPE and RRE yields were 88.56 g and 2.72 g, respectively. The extracts were stored at -20°C (Asan et al, 2019;Girsang et al, 2019;Liana et al, 2019;Vrianty et al, 2019;Widowati et al, 2018b).…”

“…Then it was incubated again in the same condition for 10 mins. The absorbances were determined at 510 nm (Asan et al, 2019;Jusri et al, 2019;Liana et al, 2019;Utami et al, 2017). The scavenging activities percentage of samples were calculated using this following formula : H 2 O 2 Scavenging Activity (%) = (Ac -As)/Ac × 100 Ac: control absorbance As: sample absorbance…”