Antioxidant and Anti-Fatigue Constituents of Okra

Xia, Fangbo; Zhong, Yu; Li, Mengqiu; Chang, Qi; Liao, Yonghong; Liu, Xinmin; Pan, Rui‐Le

doi:10.3390/nu7105435

Cited by 122 publications

(117 citation statements)

References 43 publications

(50 reference statements)

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“…The mice in all the groups were subjected to the forced swimming test 30 min after the last administration. The test was carried out as previously described, with some modification . In brief, the mice were placed in an acrylic plastic pool (50 × 40 × 50 cm) filled with fresh water (25 ± 1 °C) to a depth of 30 cm.…”

Section: Methodsmentioning

confidence: 99%

“…The test was carried out as previously described, with some modification. 34 In brief, the mice were placed in an acrylic plastic pool (50 × 40 × 50 cm) filled with fresh water (25 ± 1°C) to a depth of 30 cm. Exhaustion was defined as loss of coordinated movements and failure to rise to the surface within 7 s, and the swimming time was recorded.…”

Section: Forced Swimming Testmentioning

confidence: 99%

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Myricanol rescues dexamethasone‐induced muscle dysfunction via a sirtuin 1‐dependent mechanism

Shen

Liao

Liu

et al. 2019

J cachexia sarcopenia muscle

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Background Muscle atrophy and weakness are adverse effects of high dose or the sustained usage of glucocorticoids. Loss of mitochondria and degradation of protein are highly correlated with muscle dysfunction. The deacetylase sirtuin 1 (SIRT1) plays a vital role in muscle remodelling. The current study was designed to identify myricanol as a SIRT1 activator, which could protect skeletal muscle against dexamethasone‐induced wasting. Methods The dexamethasone‐induced atrophy in C2C12 myotubes was evaluated by expression of myosin heavy chain, muscle atrophy F‐box (atrogin‐1), and muscle ring finger 1 (MuRF1), using western blots. The mitochondrial content and oxygen consumption were assessed by MitoTracker staining and extracellular flux analysis, respectively. Muscle dysfunction was established in male C57BL/6 mice (8–10 weeks old, n = 6) treated with a relatively high dose of dexamethasone (25 mg/kg body weight, i.p., 10 days). Body weight, grip strength, forced swimming capacity, muscle weight, and muscle histology were assessed. The expression of proteolysis‐related, autophagy‐related, apoptosis‐related, and mitochondria‐related proteins was analysed by western blots or immunoprecipitation. Results Myricanol (10 μM) was found to rescue dexamethasone‐induced muscle atrophy and dysfunction in C2C12 myotubes, indicated by increased expression of myosin heavy chain (0.33 ± 0.14 vs. 0.89 ± 0.21, * P < 0.05), decreased expression of atrogin‐1 (2.31 ± 0.67 vs. 1.53 ± 0.25, * P < 0.05) and MuRF1 (1.55 ± 0.08 vs. 0.99 ± 0.12, ** P < 0.01), and elevated ATP production (3.83 ± 0.46 vs. 5.84 ± 0.79 nM/mg protein, ** P < 0.01), mitochondrial content (68.12 ± 10.07% vs. 116.38 ± 5.12%, * P < 0.05), and mitochondrial oxygen consumption (166.59 ± 22.89 vs. 223.77 ± 22.59 pmol/min, ** P < 0.01). Myricanol directly binds and activates SIRT1, with binding energy of −5.87 kcal/mol. Through activating SIRT1 deacetylation, myricanol inhibits forkhead box O 3a transcriptional activity to reduce protein degradation, induces autophagy to enhance degraded protein clearance, and increases peroxisome proliferator‐activated receptor γ coactivator‐1α activity to promote mitochondrial biogenesis. In dexamethasone‐induced muscle wasting C57BL/6 mice, 5 mg/kg myricanol treatment reduces the loss of muscle mass; the percentages of quadriceps and gastrocnemius muscle in myricanol‐treated mice are 1.36 ± 0.02% and 0.87 ± 0.08%, respectively (cf. 1.18 ± 0.06% and 0.78 ± 0.05% in dexamethasone‐treated mice, respectively). Myricanol also rescues dexamethasone‐induced muscle weakness, indicated by improved grip strength (70.90 ± 4.59 vs. 120.58 ± 7.93 g, ** P < 0.01) and prolonged swimming exhaustive time (48.80 ± 11.43 vs. 83.75 ± 15.19 s, **...

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Section: Methodsmentioning

confidence: 99%

Section: Forced Swimming Testmentioning

confidence: 99%

Myricanol rescues dexamethasone‐induced muscle dysfunction via a sirtuin 1‐dependent mechanism

Shen

Liao

Liu

et al. 2019

J cachexia sarcopenia muscle

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“…Independence activity test was performed according to the method described previously with some modification [22]. Thirty minutes after AC administration, mice were placed individually in the autonomic activities instrument (ZZ-6, Chengdu Taimeng Science Technology Co., Ltd., Chengdu, China).…”

Section: Methodsmentioning

confidence: 99%

Antifatigue Effects of Antrodia cinnamomea Cultured Mycelium via Modulation of Oxidative Stress Signaling in a Mouse Model

Liu

et al. 2017

BioMed Research International

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Antrodia cinnamomea, a folk medicinal mushroom, has numerous biological effects. In this study, we aim to assess whether the antifatigue effects of A. cinnamomea mycelia (AC) and its underlying mechanisms are related to oxidative stress signaling using behavioral mouse models and biochemical indices detection. Mice were orally treated with AC at doses of 0.1, 0.3, and 0.9 g/kg for three weeks. AC had no effect on the spontaneous activities of mice indicating its safety on central nervous system. Furthermore, results obtained from weight-loaded forced swimming test, rotary rod test, and exhausted running test confirmed that AC significantly enhanced exercise tolerance of mice. Biochemical indices levels showed that these effects were closely correlated with inhibiting the depletion of glycogen and adenosine triphosphate stores, regulating oxidative stress-related parameters (superoxide dismutase, glutathione peroxidase, reactive oxygen species, and malondialdehyde) in serum, skeletal muscle, and liver of mice. Moreover, the effects of AC may be related with its regulation on the activations of AMP-activated protein kinase, protein kinase B, and mammalian target of rapamycin in liver and skeletal muscle of mice. Altogether, our data suggest that the antifatigue properties of AC may be one such modulation mechanism via oxidative stress-related signaling in mice.

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“…Antioxidant assays, including 1-diphenyl-2-picrylhydrazyl scavenging, ferric reducing antioxidant power, reducing power test and weight-loaded swimming test showed its seeds possessed significant antioxidant and antifatigue effects. Moreover, biochemical determination revealed that anti-fatigue activity of okra seeds is caused by reducing the levels of blood lactic acid and urea nitrogen, enhancing hepatic glycogen storage, promoting anti-oxidant ability by lowering malondialdehyde level and increasing superoxide dismutase and glutathione peroxides levels [37].…”

Section: Anti-fatigue and Anti-oxidant Effectsmentioning

confidence: 99%

A Review on Ethnomedicinal, Pharmacological, Phytochemical and Pharmaceutical Profile of Lady’s Finger (<i>Abelmoschus esculentus</i> L.) Plant

Chowdhury¹,

Jamaly²,

Farjana³

et al. 2019

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Abelmoschus is a genus of about fifteen species belongs to the family Malvaceae. The herb, popularly known as Lady's Finger or Okra (in English) is a nutritional source used for both medicinal and culinary purposes. The plant is widely distributed from Africa to Asia, Southern Europe, and America. This comprehensive account provides a botanical description of the plant, its phytochemical constituents and pharmacological activities focusing anti-diabetic, anti-oxidant, anti-adhesive, gastro-protective, hepatoprotective, and immunomodulating actions. Most of the pharmacological effects can be explained by the constituents like tannins, terpenoids, flavonoids and glycosides present in all plant parts. However, future efforts should concentrate more on in vitro and in vivo studies and also on clinical trials in order to confirm traditional wisdom in the light of a rational phytotherapy. The present review is an overview of phytochemistry and ethnopharmacological studies that support many of the traditional ethnomedicinal uses of the plant.

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Antioxidant and Anti-Fatigue Constituents of Okra

Cited by 122 publications

References 43 publications

Myricanol rescues dexamethasone‐induced muscle dysfunction via a sirtuin 1‐dependent mechanism

Myricanol rescues dexamethasone‐induced muscle dysfunction via a sirtuin 1‐dependent mechanism

Antifatigue Effects of Antrodia cinnamomea Cultured Mycelium via Modulation of Oxidative Stress Signaling in a Mouse Model

A Review on Ethnomedicinal, Pharmacological, Phytochemical and Pharmaceutical Profile of Lady’s Finger (<i>Abelmoschus esculentus</i> L.) Plant

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Antioxidant and Anti-Fatigue Constituents of Okra

Cited by 122 publications

References 43 publications

Myricanol rescues dexamethasone‐induced muscle dysfunction via a sirtuin 1‐dependent mechanism

Myricanol rescues dexamethasone‐induced muscle dysfunction via a sirtuin 1‐dependent mechanism

Antifatigue Effects of Antrodia cinnamomea Cultured Mycelium via Modulation of Oxidative Stress Signaling in a Mouse Model

A Review on Ethnomedicinal, Pharmacological, Phytochemical and Pharmaceutical Profile of Lady’s Finger (&lt;i&gt;Abelmoschus esculentus&lt;/i&gt; L.) Plant

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A Review on Ethnomedicinal, Pharmacological, Phytochemical and Pharmaceutical Profile of Lady’s Finger (<i>Abelmoschus esculentus</i> L.) Plant