Antioxidant and Anti-Cataract Effects of Chlorella on Rats with Streptozotocin-Induced Diabetes

Shibata, Shinya; Natori, Yu; Nishihara, Terumi; Tomisaka, Kazue; Matsumoto, Kunihiro; Sansawa, Hiroshi; Nguyen, Van Chuyen

doi:10.3177/jnsv.49.334

Cited by 90 publications

(52 citation statements)

References 28 publications

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“…Furthermore, α and β-carotene in C. vulgaris not only react with various ROS but also interfere with the processes of oxidation in the lipid and cellular compartment [38]. Chlorella vulgar has many health-promoting benefits as antioxidant and lipid reduction in animals and humans [39] with free radical scavenging action [40,41].…”

Section: (Handex1000) C: Liver Tissue Section Of Rats Treated With Delmentioning

confidence: 99%

Hepatoprotective Impact of Chlorella vulgaris Powder on Deltamethrin Intoxicated Rats

El-Sheikh

Galal

Fadil

2018

Zagazig Veterinary Journal

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This study was conducted to evaluate the possible protective impact of Chlorella vulgaris (C. vulgaris) on liver tissues of adult male albino rats intoxicated with deltamethrin (DLM). Thirtytwo adult rats were divided into four equal groups. The control group was orally administered 0.5 mL normal saline once daily for eight weeks. The second (Chlorella group (CV)) and third (Deltamethrin group (DLM)) groups, were orally received 50 mg C. vulgaris powder/kg BW and deltamethrin 3 mg/kg BW once daily for eight weeks respectively, while the fourth group was orally received CV and DLM together, with the same previous mentioned doses and duration. DLM treated group induced a significant elevation of serum alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities. Liver oxidant/antioxidant status was altered due to DLM intoxication. Meanwhile, C. vulgaris powder normalized the changes of ALT and ALP activities. Moreover, it improved oxidant/antioxidant status of the liver related to DLM intoxication. C. vulgaris supplementation could overcome the DLM-induced hepatotoxicity through eliminating the oxidative tissue injuries.

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Section: (Handex1000) C: Liver Tissue Section Of Rats Treated With Delmentioning

confidence: 99%

Hepatoprotective Impact of Chlorella vulgaris Powder on Deltamethrin Intoxicated Rats

El-Sheikh

Galal

Fadil

2018

Zagazig Veterinary Journal

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“…Chemiluminescense (CL), generated via free radicals, is related to lipid peroxidation ( 19 ), and was determined as described by a previous study ( 18 ). The urinary 8-hydroxy-2 ′ -deoxyguanosine (8-OHdG) concentration was measured using the 8-OHdG enzyme-linked immunosorbent assay kit (Japan Institute for Control Aging, Shizuoka, Japan), and adjusted by urinary Cr values.…”

Section: Measurement Of Lipid Peroxidation and Urinary 8-hydroxy-2 ′ mentioning

confidence: 99%

“…The lipid peroxide levels in the serum were measured as the thiobarbituric acid reactive substances (TBARS) using the method of Yagi ( 17 ) as described in a previous study ( 18 ). Chemiluminescense (CL), generated via free radicals, is related to lipid peroxidation ( 19 ), and was determined as described by a previous study ( 18 ).…”

Section: Measurement Of Lipid Peroxidation and Urinary 8-hydroxy-2 ′ mentioning

confidence: 99%

Effect of Astaxanthin in Combination with .ALPHA.-Tocopherol or Ascorbic Acid against Oxidative Damage in Diabetic ODS Rats

Nakano

Onodera

Saito

et al. 2008

J Nutr Sci Vitaminol

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SummaryThe present study was performed to investigate the effect of astaxanthin in combination with other antioxidants against oxidative damage in streptozotocin (STZ)-induced diabetic Osteogenic Disorder Shionogi (ODS) rats. Diabetic-ODS rats were divided into five groups: control, astaxanthin, ascorbic acid, ␣ -tocopherol, and tocotrienol. Each of the four experimental groups was administered a diet containing astaxanthin (0.1 g/kg), in combination with ascorbic acid (3.0 g/kg), ␣ -tocopherol (0.1 g/kg), or tocotrienol (0.1 g/ kg) for 20 wk. The effects of astaxanthin with other antioxidants on lipid peroxidation, urinary 8-hydroxy-2 ′ -deoxyguanosine (8-OHdG) excretion, serum creatinine (Cr) level, creatinine clearance (Ccr), and urinary protein content were assessed. The serum lipid peroxide levels and chemiluminescent (CL) intensity in the liver of the ␣ -tocopherol and tocotrienol groups were significantly reduced in comparison to that of the control group. In the ␣ -tocopherol group, urinary 8-OHdG excretion, serum Cr level, Ccr, urinary albumin excretion, and urinary protein concentration were significantly decreased as compared with those in the control group. Additionally, the CL intensity in the kidney of the ␣ -tocopherol group was significantly lower, but that of the ascorbic acid group was significantly higher than that in the control group. These results indicate that dietary astaxanthin in combination with ␣ -tocopherol has an inhibitory effect on oxidative stress. On the other hand, our study suggests that excessive ascorbic acid intake increases lipid peroxidation in diabetic rats.

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“…Some Chlorella species are widely used as a dietary supplement in many countries, especially in East Asia (e.g., Japan, Taiwan, Korea). Chlorella regularis has been reported to potentially prevent lifestylerelated diseases (Shibata et al, 2003;Shibata and Sansawa, 2006;Shibata et al, 2007). Chlorella powder (CP) made from C. regularis contains large amounts of carotenoids such as lutein, α-carotene, and β-carotene (approx.…”

Section: Introductionmentioning

confidence: 99%

Bioavailability of Lutein in Chlorella Powder: A Single Ingestion of Chlorella Powder Raises Serum Lutein Concentrations in Healthy Human Volunteers

Shibata

Hayakawa

2009

FSTR

Self Cite

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Chlorella powder (CP), a dietary supplement made from a green alga, contains abundant lutein (2.8 mg/g), but the bioavailability of lutein in CP has not been evaluated. In the present study, we assessed the effect of a single ingestion of CP (3 or 6 g) in tablet form on serum lutein concentrations in humans (n = 21). At 1 d, serum lutein concentrations increased from baseline values by 34% (25.6 nM/mg-lutein intake) after 3 g CP and by 66% (21.4 nM/mg-lutein intake) after 6 g CP. These results suggest that lutein in CP is highly bioavailable.Keywords: bioavailability, Chlorella, green alga, lutein *To whom correspondence should be addressed. E-mail: shinya-shibata@yakult.co.jp IntroductionLutein is the most abundant carotenoid in nature, and green leafy vegetables are the most popular source of dietary lutein. Lutein and zeaxanthin exist in the lens of the eye and the macular region of the retina (Krinsky et al., 2003). Many studies have indicated that the consumption of lutein-and zeaxanthin-rich food is associated with a reduced risk of agerelated macular degeneration (AMD) and cataracts (Moeller et al., 2000;Seddon et al., 1994). Seddon et al. (1994) have reported that daily intake of 6 mg of lutein was associated with a reduced risk of AMD. Richer et al. (2004) reported that a daily intake of 10 mg of lutein improved the vision of AMD patients.During the absorption of carotenoids from food, the carotenoids are initially transferred from the intact food matrix into emulsified lipid droplets. Subsequently, they are mixed into micelles, as are other dietary lipids, and then absorbed by the small intestine. These processes are influenced by various characteristics of the food; for example, fat facilitates the absorption of carotenoids, whereas dietary fiber inhibits it (van het Hof et al., 2000). Thus, to consume adequate amounts of vegetables and fruits in order to ensure sufficient intake of carotenoids, we must determine the bioavailability of carotenoids in food, not just the carotenoid content of the food.Chlorella species are unicellular green algae with high photosynthetic efficiency, and are rich in protein, essential nutrients, and plant pigments. Some Chlorella species are widely used as a dietary supplement in many countries, especially in East Asia (e.g., Japan, Taiwan, Korea). Chlorella regularis has been reported to potentially prevent lifestylerelated diseases (Shibata et al., 2003;Shibata and Sansawa, 2006;Shibata et al., 2007). Chlorella powder (CP) made from C. regularis contains large amounts of carotenoids such as lutein, α-carotene, and β-carotene (approx. 2.5 mg/g, 250 μg/g, and 500 μg/g, respectively). Thus, the ingestion of CP could improve low dietary intake of lutein due to low consumption of vegetables and fruits, because only a few grams of CP can supply 6 to 10 mg of lutein. However, to our knowledge, there is no available information about carotenoid bioavailability of CP.The aim of the present study was to determine the bioavailability of lutein from CP in humans. We assessed the e...

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Antioxidant and Anti-Cataract Effects of Chlorella on Rats with Streptozotocin-Induced Diabetes

Cited by 90 publications

References 28 publications

Hepatoprotective Impact of Chlorella vulgaris Powder on Deltamethrin Intoxicated Rats

Hepatoprotective Impact of Chlorella vulgaris Powder on Deltamethrin Intoxicated Rats

Effect of Astaxanthin in Combination with .ALPHA.-Tocopherol or Ascorbic Acid against Oxidative Damage in Diabetic ODS Rats

Bioavailability of Lutein in Chlorella Powder: A Single Ingestion of Chlorella Powder Raises Serum Lutein Concentrations in Healthy Human Volunteers

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