Antioxidant and Anti‐Cancer Potentials of Rheum emodi Rhizome Extracts

Abstract

The objective of this study was to determine antioxidant and cytotoxic efficacies of methanolic and aqueous extracts of Rheum emodi Wall. ex Meissn. rhizome. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging activities, inhibitory effect on lipid peroxidation and Fe3+ reducing antioxidant property have been used to investigate antioxidant properties of the extracts. Cytotoxicity of the extracts was tested on MDA-MB-435S and Hep3B cell lines. Both extracts displayed extensive cytotoxicity to the tested cell lines. The extracts were studied for their ability to protect pBR322 DNA from damage by UV induced photolysis of H2O2. The aqueous extract, though inferior to methanolic extract in its antioxidant potential exhibited efficiency in DNA protection, while the methanolic extract failed to protect the DNA. The amount of total polyphenolics in the extracts was measured by spectrophotometric method. The methanolic extract contained higher polyphenolic contents than aqueous extract. Significant positive correlations were observed (P < .05) between results of phenolic content estimation and that of antioxidant assays. Hence, high-performance liquid chromatography analysis was performed to identify few major phenolic compounds that might be responsible for these therapeutic properties. These results indicate that rhizome of R. emodi possesses antioxidant and cytotoxic activities and therefore have therapeutic potential.

Novel platinum&ndash;palladium bimetallic nanoparticles synthesized by Dioscorea bulbifera: anticancer and antioxidant activities

“…Reactive oxygen species production is found to be extensive in tumor cells, which upregulates certain growth factors and matrix metalloproteinases, promoting angiogenesis, metastasis, and secondary tumor developments. 41 In our study we found that the nanoparticles were not only cytotoxic against the cancer cells, but also possess antioxidant properties. It was evident that the free radical scavenging potential increased in the bimetallic nanoparticles (Pt-PdNPs) as compared to individual NPs.…”

Cytology of Rheum, a vulnerable medicinal plant from Kashmir Himalaya

“…The root powder is sprinkled on ulcers and wounds for quick healing and also is used for cleaning teeth (Chaterjee and Pakrash 1991;Kaul 1997). It is given in small doses against indigestion and abdominal disorders (Ballah and Churasia 2009).The rhizomes are a potential source of anticancer drug (Rajkumar et al 2010). The genus is also reported to have anti-microbial, anti-oxidant and anti-diabetic properties (Babu et al 2003, Matsuda et al 2001Radika et al 2010).…”

Total Phenolic Content and Antioxidant Activity of Standardized Extracts from Leaves and Cell Cultures of Three Callistemon Species

“…Polyphenolic compounds are also believed to have chemopreventive and suppressive activities against cancer cells by inhibition of metabolic enzymes involved in the activation of potential carcinogens or arresting the cell cycle [19]. Nevertheless, a compound with strong antioxidant potential can also contribute to DNA protection and prevent apoptosis [20]. Further studies are therefore required to detect potential anticancer activities of the extracts reported here.…”