Antioxidant and anti cancer activities of brown and red seaweed extracts from Chilean coasts

Miranda-Delgado, Alejandra; Montoya, María; Paz-Araos, Marilyn; Mellado, Marco; Villena, Joan; Arancibia, Paulina; Madrid, Alejandro

doi:10.3856/vol46-issue2-fulltext-6

Cited by 19 publications

(10 citation statements)

References 53 publications

(79 reference statements)

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“…Our results, therefore, are in support of the lipophilic nature of D. dichotoma cytotoxic agents, whereby the cytotoxic activity of several algae was attributed to the presence of several nonpolar compounds particularly in the chloroform fraction [26,[30][31][32]. Other studies also showed that the nonpolar petroleum ether extract [33] as well as dichloromethane [34] of the algae were significantly effective against proliferating cells.…”

Section: Cytotoxic Activity Against Cancer Cell Linessupporting

confidence: 83%

“…dichotoma are yet to be elucidated, many researchers believe that the wide array of diterpenes found in brown algae are involved [16,[34][35][36]. Hydroazulene diterpenes isolated from D. dichotoma were reported to be significantly cytotoxic to the cancerous murine cell line KA3IT [33].…”

Section: Cytotoxic Activity Against Cancer Cell Linesmentioning

confidence: 99%

“…Diterpenoids within D. dichotoma were reported to exert a potent antioxidant effect as assessed by the ABTS and erythrocytes hemolysis assays [36], whereas fucoxanthin was an effective radical scavenger, exhibiting 13.5 times higher hydroxyl radical scavenging activity compared to that of vitamin E [46]. With the reported lack of correlation between the various methods used to evaluate the antioxidant capabilities of extracts [34], the use of DPPH assay on its own in our study limits the ability to propose a perspective of the mechanism of the observed modest antioxidant activity of our crude extract.…”

Section: Antioxidant Activitymentioning

confidence: 99%

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The Yemeni Brown AlgaeDictyota dichotomaExhibit HighIn VitroAnticancer Activity Independent of Its Antioxidant Capability

El-Shaibany

Al‐Habori

Al-Maqtari

et al. 2020

BioMed Research International

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e aim of this study was to investigate the anticancer and antioxidant activities as well as the safety of the brown algae Dictyota dichotoma of the Western seacoast of Yemen. Cytotoxicity of methanol extract of D. dichotoma and several of its fractions, petroleum ether, chloroform, ethyl acetate, n-butanol, and aqueous extracts against seven different cancer cell lines was determined by crystal violet staining. e antioxidant activity was also assessed using the DPPH radical scavenging assay. Acute toxicity study was performed on rats at increasing doses of the methanol extract. Extracts of D. dichotoma exerted a significant dose-dependent cytotoxicity on the seven tumor cell lines but were generally more selective on MCF-7 and PC-3. Among all fractions, the chloroform fraction of the D. dichotoma displayed the highest cytotoxic activity and was most effective in MCF-7, PC3, and CACO cells (IC 50 � 1.93 ± 0.25, 2.2 ± 0.18, and 2.71 ± 0.53 μg/mL, respectively). e petroleum ether fraction was also effective, particularly against MCF-7 and PC-3 (IC 50 � 4.77 ± 0.51 and 3.93 ± 0.51 μg/mL, respectively) whereas the activity of the ethyl acetate fraction was more pronounced against HepG2 and CACO (IC 50 � 5.06 ± 0.21 and 5.06 ± 0.23 μg/mL, respectively). Of all the extracts tested, the crude methanolic extract of the algae exhibited only a modest antioxidant potential (IC 50 � 204.6 ± 8.3 μg/mL). Doses as high as 5000 mg/kg body weight of D. dichotoma methanolic extracts were safe and well tolerated by rats. e overall results showed that D. dichotoma exhibited a significant cytotoxic activity probably due to the occurrence of nonpolar cytotoxic compounds, which is independent of its antioxidant capability.

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Section: Cytotoxic Activity Against Cancer Cell Linessupporting

confidence: 83%

Section: Cytotoxic Activity Against Cancer Cell Linesmentioning

confidence: 99%

Section: Antioxidant Activitymentioning

confidence: 99%

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The Yemeni Brown AlgaeDictyota dichotomaExhibit HighIn VitroAnticancer Activity Independent of Its Antioxidant Capability

El-Shaibany

Al‐Habori

Al-Maqtari

et al. 2020

BioMed Research International

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“…20,21 This method has been widely used to evaluate antioxidant activities and has advantages over the other in vitro assays, including DPPH, ferric reducing antioxidant power (FRAP), and trolox equivalent antioxidant capacity (TEAC). 2,[22][23] Keratinocytes are the major cells that make up the skin epidermis, which have a similar topology as the outer layer of the skin. Therefore, the antioxidant assay on HaCaT cells is more representative than the other in vitro methods.…”

Section: Antioxidant Activity Of Merbaumentioning

confidence: 99%

**Protective Effect of Merbau (Intsia bijuga) Extract on Hydrogen Peroxide-Treated HaCaT Human Keratinocytes and Its Formulation as Antioxidant Cream**

et al. 2020

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Background: The excessive generation of ROS in the skin results in oxidative stress that can contribute to premature skin aging, inflammation, and skin carcinogenesis. To prevent these detrimental effects, the development of herbal medicine with a potent antioxidant activity into cosmetic products is required. This study aims to formulate cream that contains a safe and effective concentration of merbau (Intsia bijuga), which has been shown to have a strong antioxidant activity. Methods: Powdered merbau wood was macerated with methanol and the dried extract was evaluated for its cytotoxic effect and antioxidant activity on human keratinocytes cell line using MTS assay. Five cream formulations containing the extract were made and subjected to stability and physical evaluations, including organoleptic, types of cream, pH, viscosity, and homogeneity. Results: Cytotoxicity assay revealed that merbau extracts had an IC50 of 181.3 μg/mL (95% confidence interval (CI): 165.4 - 200.1 µg/mL). At a concentration of 31.25 μg/mL, the extract exhibited a protective effect against H2O2-induced oxidative stress, comparable to vitamin E. Five cream formulas that were developed demonstrated good physical properties that fulfilled the evaluation parameters, including o/w type of cream, homogenous, and stable based on centrifugation and freeze-thaw cycle tests. The pH values were between 5.65 ± 0.067 - 7.4 ± 0.050, while the viscosity values were between 131 ± 1.249 - 56,011 ± 2,729.27 mPa.s. All cream formulas exhibited shear-thinning properties upon increasing shear stress. Conclusion: Overall, this study has successfully formulated several cream formulations containing merbau extract at a concentration that shows antioxidant activity.

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“…For example, fucoxanthin, dimethylsultonipropionate have been described in Fucus vesiculosus as antibacterials (Saha et al, 2011;Svensson et al, 2013); and porphyrans and other polysaccharides have been characterized as antioxidant compounds in species of the genera Porphyra and Pyropia (Zhang et al, 2003;Zhao et al, 2006;Isaka et al, 2015). Recently, the presence of polyphenols, flavonoids and anthraquinones has also been demonstrated, which would have an antioxidant and anti-cancer activity in the seaweeds Desmarestia ligulata, Dictyota kunthii, Chondracanthus chamissoi and Laurencia chilensis (Miranda et al, 2018). In this context, our purpose was to identify compounds associated to desiccation stress tolerance mechanisms, but also those having a potential role as bioactive compounds under desiccation.…”

Section: Introductionmentioning

confidence: 99%

First approach of characterization of bioactive compound in Pyropia orbicularis during the daily tidal cycle

Latorre¹,

Castañeda²,

Meynard³

et al. 2019

LAJAR

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The red seaweed Pyropia orbicularis is an economic important species that occurs predominantly in the high intertidal zone along the chilean coast where it experiences extreme conditions under daily turning tides. Its gametophytic phase has been demonstrated to have a high desiccation tolerance, both at the genomic and proteomic levels, but studies at the metabolomic level are still lacking. This research aimed at characterizing compounds related to desiccation stress tolerance by performing several phase solid extractions with different solvents. Bioactivity-guided fractionation (antioxidant and antibacterial activities) was made for a more specific characterization. Compounds identification was done using LC-MS/MS. Results showed that P. orbicularis produces different compounds depending on the state of hydration during the tidal cycle. For example, minoxidil was only found under hydration, while vincamine only during desiccation. It was also found that the main antioxidant activity was most likely due to lutein and the antibacterial activity could be mainly attributed to compounds of lipid nature such as phosphatidylethanolamine (PE), phosphatidylserine (PS) and monogalactosyldiacylglycerol (MGDG). These results help to get a better understanding of the stress tolerance mechanisms in P. orbicularis and place it like a potential source of bioactive compounds.

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Antioxidant and anti cancer activities of brown and red seaweed extracts from Chilean coasts

Cited by 19 publications

References 53 publications

The Yemeni Brown AlgaeDictyota dichotomaExhibit HighIn VitroAnticancer Activity Independent of Its Antioxidant Capability

The Yemeni Brown AlgaeDictyota dichotomaExhibit HighIn VitroAnticancer Activity Independent of Its Antioxidant Capability

**Protective Effect of Merbau (Intsia bijuga) Extract on Hydrogen Peroxide-Treated HaCaT Human Keratinocytes and Its Formulation as Antioxidant Cream**

First approach of characterization of bioactive compound in Pyropia orbicularis during the daily tidal cycle

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