Antioxidant and angiotensin I-converting enzyme (ACE) inhibitory peptides of rainbow trout (Oncorhynchus mykiss) viscera hydrolysates subjected to simulated gastrointestinal digestion and intestinal absorption

Vásquez, Priscilla; Montoya, José Edgar Zapata; Chamorro, Verónica Celeste; Fillería, Susan F. García; Tironi, Valeria Anahí

doi:10.1016/j.lwt.2021.112834

Cited by 33 publications

(17 citation statements)

References 35 publications

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“…At 3.0 mg/mL, the ACEi rate of MSCH-1b was 76.25 ± 3.61%, which was markedly stronger than those of MSCH-1 (62.97 ± 2.39%), MSCH-1a (43.57 ± 1.59%), and MSCH-1c (35.24 ± 2.21%), respectively (Figure 2B) (P < 0.05). Gel filtration is a popular method to collect fractions with a particular MW dimension from a complex mixture and is generally applied in group isolation of protein hydrolysates from different sea food, such as by-catch shrimp waste (Joshi et al, 2020), miiuy croaker muscle (He et al, 2019b), skipjack tuna byproducts (Yang et al, 2019a;Wang et al, 2022a), red stingray (Dasyatis akajei) cartilages (Pan et al, 2019), rainbow trout viscera (Vaśquez et al, 2022), Antarctic krill (Zhao et al, 2019b), and three-spot seahorse (Shi et al, 2020). In the experiment, MSCH-1b displayed the best ACEi activity, but it does not obtain the lowest MW.…”

Section: Preparing Acei Peptides From Mschmentioning

confidence: 99%

“…Those fish byproducts will lead to serious environmental problems if they can't be handled properly (Zhang et al, 2019;Yang et al, 2019a). To make full use of these fish byproducts, many ACEi peptides were prepared from different processing byproducts, such as tuna black muscle and bone (Lee et al, 2010;Qiao et al, 2022), smooth-hound viscera (Abdelhedi et al, 2017;Abdelhedi et al, 2018), cuttlefish muscle (Balti et al, 2015), Nile tilapia skeleton (Borges-Contreras et al, 2019), rainbow trout viscera (Vaśquez et al, 2022) [34], and Alaska pollack skins (Yang et al, 2021). Those sea-food derived ACEi peptides exhibit high potential application value in diet or clinical treatment on antihypertension (Abdelhedi and Nasri, 2019;Fan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Isolation, identification, molecular docking analysis, and cytoprotection of seven novel angiotensin I-converting enzyme inhibitory peptides from miiuy croaker byproducts-swim bladders

et al. 2022

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For efficiently utilizing the processing byproducts of miiuy croaker to prepare novel angiotensin I-converting enzyme (ACE) inhibitory (ACEi) peptides, in vitro gastrointestinal (GI) digestion method was screened and employed to prepare swim bladder hydrolysate with the highest ACEi activity. Subsequently, seven novel ACEi peptides were isolated from the hydrolysate and identified as DEGPE, EVGIQ, SHGEY, GPWGPA, GPFGTD, SPYGF, and VIGPF with molecular weights of 545.49, 544.58, 591.55, 583.63, 592.59, 569.60, and 531.63 Da, respectively. SHGEY and SPYGF exhibited remarkable ACEi activity with IC50 values of 0.86 ± 0.12 and 0.37 ± 0.06 mg/mL. Molecular docking experiment illustrated that the significant ACEi activity of SHGEY and SPYGF with the affinity of -8.7 and -9.7 kcal/mol mainly attributed to effectively combining with the ACEi active sites by hydrophobic interaction, electrostatic force and hydrogen bonding. Moreover, SHGEY and SPYGF could significantly up-regulate the nitric oxide (NO) production and decrease the endothelin-1 (ET-1) secretion in human umbilical vein endothelial cells (HUVECs), but also abolished the negative impacting of norepinephrine to the levels of NO and ET-1. Furthermore, SHGEY and SPYGF showed significant protection to HUVECs against H2O2 damage by increasing superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity to lower the contents of reactive oxide species and malondialdehyde. Consequently, ACEi peptides derived from miiuy croaker swim bladders, especially SHGEY and SPYGF, are health-promoting ingredients for functional products as a supplementary treatment to hypertension and cardiovascular diseases.

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Section: Preparing Acei Peptides From Mschmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation, identification, molecular docking analysis, and cytoprotection of seven novel angiotensin I-converting enzyme inhibitory peptides from miiuy croaker byproducts-swim bladders

et al. 2022

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“…Therefore, searching for natural and safer ACEi molecules for alternative chemosynthetic drugs is vital for hypertension and CVD therapy. Currently, ACEi peptides have been prepared from a variety of marine protein resources, such as skate skin ( 36 ), mackerel skin ( 37 ), flounder fish muscle ( 38 ), smooth-hound viscera ( 39 ), Antarctic krill ( 34 ), stonefish ( 40 ), shrimp waste ( 41 ), rainbow trout viscera ( 42 ), and lizardfish ( 43 ). These ACEi peptides exhibit not only high nutritional value but also a significant biological activity for application in diet or clinical treatment on antihypertension ( 44 – 46 ).…”

Section: Introductionmentioning

confidence: 99%

Preparation, Characterization, and Cytoprotective Effects on HUVECs of Fourteen Novel Angiotensin-I-Converting Enzyme Inhibitory Peptides From Protein Hydrolysate of Tuna Processing By-Products

et al. 2022

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To effectively utilize skipjack tuna (Katsuwonus pelamis) processing by-products to prepare peptides with high angiotensin-I-converting enzyme (ACE) inhibitory (ACEi) activity, Neutrase was selected from five kinds of protease for hydrolyzing skipjack tuna dark muscle, and its best hydrolysis conditions were optimized as enzyme dose of 1.6%, pH 6.7, and temperature of 50°C using single factor and response surface experiments. Subsequently, 14 novel ACEi peptides were prepared from the high ACEi protein hydrolysate and identified as TE, AG, MWN, MEKS, VK, MQR, MKKS, VKRT, IPK, YNY, LPRS, FEK, IRR, and WERGE. MWN, MEKS, MKKS, and LPRS displayed significantly ACEi activity with IC50 values of 0.328 ± 0.035, 0.527 ± 0.030, 0.269 ± 0.006, and 0.495 ± 0.024 mg/mL, respectively. Furthermore, LPRS showed the highest increasing ability on nitric oxide (NO) production among four ACEi peptides combining the direct increase and reversing the negative influence of norepinephrine (NE), and MKKS showed the highest ability on directly decreasing and reversing the side effects of NE on the secretion level of endothelin-1 (ET-1) among four ACEi peptides. These findings demonstrate that seafood by-product proteins are potential ACEi peptide sources and prepared ACEi peptides from skipjack tuna dark muscle, which are beneficial components for functional food against hypertension and cardiovascular diseases.

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“…Due to mild process conditions and easy control of desired reactions, enzymatic hydrolysis is an efficient method to release bioactive peptides from protein molecules. Currently, several protein hydrolysates, including adzuki bean [ 3 ], hemp bran [ 4 ], rainbow trout viscera [ 5 ], fish sauce by-products [ 6 ], and sturgeon skin [ 7 ], have been reported to exhibit both ACE inhibition and antioxidant activity.…”

Section: Introductionmentioning

confidence: 99%

Effects of Spray Drying and Freeze Drying on Physicochemical Properties, Antioxidant and ACE Inhibitory Activities of Bighead Carp (Aristichthys nobilis) Skin Hydrolysates

Dong

Yan

Zhang

2022

Foods

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The physicochemical, structural properties, antioxidant, and angiotensin I-converting enzyme (ACE) inhibitory activities of fish skin protein hydrolysate (SPH) that were freeze-dried (SPH-FD) and spray-dried (SPH-SD) were investigated. SPH-SD showed abundant volatile compounds, higher DPPH radical scavenging activity and ferrous iron chelating activity than SPH-FD, while the ABTS radical scavenging activity and ACE inhibitory activity were not influenced by the drying method. Amino acid compositions showed a higher proportion of proline and hydroxyproline residues in SPH-FD. The major molecular weights were both distributed below 1000 Da. SPH-SD had spherical structures, while SPH-FD had glass shard-like structures. The results indicated that the drying method could affect the physicochemical properties of hydrolysates, and SPH-SD showed potential prospects in developing functional fortified foods.

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Antioxidant and angiotensin I-converting enzyme (ACE) inhibitory peptides of rainbow trout (Oncorhynchus mykiss) viscera hydrolysates subjected to simulated gastrointestinal digestion and intestinal absorption

Cited by 33 publications

References 35 publications

Isolation, identification, molecular docking analysis, and cytoprotection of seven novel angiotensin I-converting enzyme inhibitory peptides from miiuy croaker byproducts-swim bladders

Isolation, identification, molecular docking analysis, and cytoprotection of seven novel angiotensin I-converting enzyme inhibitory peptides from miiuy croaker byproducts-swim bladders

Preparation, Characterization, and Cytoprotective Effects on HUVECs of Fourteen Novel Angiotensin-I-Converting Enzyme Inhibitory Peptides From Protein Hydrolysate of Tuna Processing By-Products

Effects of Spray Drying and Freeze Drying on Physicochemical Properties, Antioxidant and ACE Inhibitory Activities of Bighead Carp (Aristichthys nobilis) Skin Hydrolysates

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