Antioxidant and Amine Oxidase Inhibitory Activities of Hydroxyurea

Liu, Yuh Hwa; Wu, Wen Chun; Lu, Yeh Lin; Lai, Ying Jang; Hou, Wen Chi

doi:10.1271/bbb.100096

Cited by 13 publications

(13 citation statements)

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“…l-Aspartic acid β-hydroxamate and l-glutamic acid γ-hydroxamate were reported to exhibit antioxidant and angiotensin-converting enzyme inhibitory activities (Liu et al 2004), while hydroxyurea exhibited antioxidant and amine oxidase inhibitory activities (Liu et al 2010). The nicotinic acid hydroxamate showed higher anti-monophenolase and anti-diphenolase activities of mushroom TYR than kojic acid, and also showed antipigmentation activity in murine melanoma B16F10 cells (Lin et al 2012a, b).…”

Section: Introductionmentioning

confidence: 99%

Glycine hydroxamate inhibits tyrosinase activity and melanin contents through downregulating cAMP/PKA signaling pathways

Lin

et al. 2014

Amino Acids

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Among the eight amino acid hydroxamates tested, Glycine hydroxamate (GH) was the best inhibitor of mushroom tyrosinase (TYR). With L-tyrosine as substrate, the GH inhibition of the monophenolase activity of the mushroom TYR was noncompetitive. GH decreased not only TYR protein expression, but also melanin content, tyrosinase-related protein (TRP)-1, TRP-2, and microphthalmia-associated transcription factor (MITF) expression in B16F10 melanoma cells while in the presence of α-melanocyte-stimulating hormone (α-MSH). GH also significantly decreased the isobutylmethylxanthine (IBMX)-induced increase in melanin content, which was not prevented by the ERK inhibitor PD98059. These results suggest GH has the potential for use in cosmetic hypopigmentation.

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Section: Introductionmentioning

confidence: 99%

Glycine hydroxamate inhibits tyrosinase activity and melanin contents through downregulating cAMP/PKA signaling pathways

Lin

et al. 2014

Amino Acids

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“…The relationships between calculated DPPH radical scavenging activities (Y) and the acetH concentrations (X) used were transformed into the equation: Y =2.166X−3.403−0.018X 2 ( R 2 =0.999). Therefore, the calculated IC 50 of acetH for DPPH radical scavenging activity was 34.86 μM (Figure 3A), as compared with 13.1 μM for ascorbic acid21,22 and 23 μM for HU 18Figure 3B shows the hydroxyl radical scavenging activities of acetH by ESR spectrometer; the hydroxyl radical is trapped by DMPO and intensities of the DMPO-OH adducts in the blank are recognized as 100.…”

Section: Resultsmentioning

confidence: 95%

“…Therefore, following previous reports,18,21,22 the effects of acetH on SSAO inhibitory activities were investigated. The hydrogen peroxide generated was determined by using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)/HRP systems, and the changes in absorbance at 420 nm were recorded during 1 min and expressed as SSAO inhibition (%).…”

Section: Methodsmentioning

confidence: 98%

“…The commercial bovine plasma product (P-4639, Sigma Chemical Co.) was reported to contain SSAO activities 18,21,22. Therefore, following previous reports,18,21,22 the effects of acetH on SSAO inhibitory activities were investigated.…”

Section: Methodsmentioning

confidence: 99%

“…Acetohydroxamic acid (acetH, CH 3 CONHOH) is the active drug in Lithostat ® , which is used to treat urinary tract infections 17. Hydroxyurea (HU, NH 2 CONHOH), or hydroxycarbamide, is a drug (Hydrea ® , Droxia™, or Mylocel™) used to treat antineoplasm or sickle cell diseases 18. AcetH and HU are both hydroxamic acid derivatives.…”

Section: Introductionmentioning

confidence: 99%

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Antiglycation, radical scavenging, and semicarbazide-sensitive amine oxidase inhibitory activities of acetohydroxamic acid in vitro

Liu¹,

Liu

et al. 2017

DDDT

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Advanced glycation endproducts (AGEs) can promote intracellular reactive oxygen species production, and the levels of AGEs are highly correlated with cardiovascular disease and diabetes complications. Acetohydroxamic acid (acetH) is a bacterial urease inhibitor drug used to treat kidney stones and infections in the urinary tract, and hydroxyurea (HU) is a drug used for antineoplasm and sickle cell diseases. Both acetH and HU are hydroxamic acid derivatives. It was found that acetH and HU at 2.5 or 5 mM showed anti-AGE formation by lowering the AGEs’ fluorescent intensities and Nε-(carboxymethyl)lysine formation in bovine serum albumin/galactose models, and both showed better and significant differences (P<0.05) compared to the positive control of aminoguanidine. Regarding radical scavenging activities, the half-inhibition concentrations (IC50) of acetH against α,α-diphenyl-β-picrylhydrazyl radical and hydroxyl radical were 34.86 and 104.42 μM, respectively. The IC50 of acetH against semicarbazide-sensitive amine oxidase was 10.56 μM, and acetH showed noncompetitive inhibition respective to the substrates (benzylamine). The antiglycation, antioxidant, and semicarbazide-sensitive amine oxidase inhibitory activities of acetH prove that it has the potential for treating cardiovascular disease and diabetes complications and it needs further investigation in animal models.

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Tiliroside, a glycosidic flavonoid, inhibits carbohydrate digestion and glucose absorption in the gastrointestinal tract

Goto

Horita

Nagai

et al. 2011

Molecular Nutrition Food Res

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Scope: Recent studies have reported that tiliroside, a glycosidic flavonoid, possesses antidiabetic activities. In the present study, we investigated the effects of tiliroside on carbohydrate digestion and absorption in the gastrointestinal tract. Methods and results: This study showed that tiliroside inhibits pancreatic a-amylase (IC 50 5 0.28 mM) in vitro. Tiliroside was found as a noncompetitive inhibitor of a-amylase with K i values of 84.2 mM. In male ICR mice, the increase in postprandial plasma glucose levels was significantly suppressed in the tiliroside-administered group. Tiliroside treatment also suppressed hyperinsulinemia after starch administration. Tiliroside administration inhibited the increase of plasma glucose levels in an oral glucose tolerance test, but not in an intraperitoneal glucose tolerance test. In human intestinal Caco-2 cells, the addition of tiliroside caused a significant dose-dependent inhibition of glucose uptake. The inhibitory effects of both sodium-dependent glucose transporter 1 (SGLT1) and glucose transporter 2 (GLUT2) inhibitors (phlorizin and phloretin, respectively) on glucose uptake were significantly inhibited in the presence of tiliroside, suggesting that tiliroside inhibited glucose uptake mediated by both SGLT1 and GLUT2. Conclusions: These findings indicate that the anti-diabetic effects of tiliroside are at least partially mediated through inhibitory effects on carbohydrate digestion and glucose uptake in the gastrointestinal tract.

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Antioxidant and Amine Oxidase Inhibitory Activities of Hydroxyurea

Cited by 13 publications

References 50 publications

Glycine hydroxamate inhibits tyrosinase activity and melanin contents through downregulating cAMP/PKA signaling pathways

Glycine hydroxamate inhibits tyrosinase activity and melanin contents through downregulating cAMP/PKA signaling pathways

Antiglycation, radical scavenging, and semicarbazide-sensitive amine oxidase inhibitory activities of acetohydroxamic acid in vitro

Tiliroside, a glycosidic flavonoid, inhibits carbohydrate digestion and glucose absorption in the gastrointestinal tract

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