Antioxidant Analysis of Sukun (Artocarpus Altilis (Parkinson) Fosberg) Leaves Using DPPH and IC50 Methods

Abstract

Sukun (Artocarpus altilis (Parkinson) Fosberg) are abundantly found in Indonesia. However, the usage of it was very limited. This research was conducted to analyze the antioxidant activity of Sukun leaves as one of the underused plants in Indonesia. Mature Sukun leaves were extracted using maceration method where 96% technical ethanol was used as the solvent. DPPH analysis was conducted using Burdan and Olezek method (2001). IC50 method was performed to analyze Vit C content of Sukun leaves. The analysis was performed on 2, 3, 4, 5, and 6 ppm concentrations. By using the DPPH method, it was found that the total antioxidant activity of the extract was 45.79; 48.11; 49.38; 50.95; and 51.70 respectively. IC 50 analysis shows that the vitamin C content of the extract was 54.82; 57.72; 60.64; 63.94; and 65.49 respectively for every concentration. It can be concluded that Sukun leaves showed strong antioxidant activity.
 Keywords: antioxidant analysis, Sukun (Artocarpus altilis), nutrition analysis, underused plant