Antioxidant Activity of the Sea Bird Nest (Eucheuma Cottonii) and Its Radical Scavenging Effect on Human Keratinocytes

Lim, Chooi Ling; Koh, Rhun Yian; Haw, Tatt Yhew; Boudville, Laura A.

doi:10.12720/jomb.4.6.461-465

Cited by 9 publications

(9 citation statements)

References 25 publications

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“…Similarly, the vascular ROS, especially superoxide anion was elevated in aorta of db/db mice and aorta exposed to high glucose and HBN decreased the superoxide production in the high-glucose exposed tissues, indicating HBN protects against endothelial dysfunction by inhibiting ROS production. This is in agreement with previous researches that showed edible bird's nest ameliorated oxidative stress by reducing production of ROS in SH-SY5Y cells and human keratinocytes (Yew et al, 2014;Hou et al, 2015;Lim et al, 2015).…”

Section: Discussionsupporting

confidence: 93%

Edible Bird’s Nest Protects Against Hyperglycemia-Induced Oxidative Stress and Endothelial Dysfunction

et al. 2020

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Increased oxidative stress by hyperglycemia is a major cause of vascular complications in diabetes. Bird's nest, which is made from the saliva of swiftlets has both medicinal and nutritional values dated back to ancient China. However, its role in improving endothelial dysfunction due to diabetes is yet to be elucidated. The present study examined the protective effect and mechanism of action of the aqueous extract of hydrolyzed edible bird nest (HBN) on endothelium in models of diabetes, in vitro and in vivo. Male db/m+ and db/ db mice were orally administered with or without HBN and glibenclamide for 28 days, followed by vascular reactivity studies in mouse aortas. Human umbilical vein endothelial cells (HUVECs) and isolated mouse aorta from C57BL/6J were treated with high glucose (HG), HBN, sialic acid (SA), glibenclamide, and apocynin, respectively. The effects of HBN on reactive oxygen species (ROS) production and nitric oxide (NO) bioavailability were assessed by Western blot, 2′,7′-dichlorofluorescin-diacetate (DCF-DA), and 4-amino-5methylamino-2′,7′ difluorofluorescein (DAF-FM DA) in HUVECs, isolated mouse aorta, and db/db diabetic mice. HBN significantly reversed the endothelial dysfunction in diabetic mice and isolated mouse aorta. HBN normalized ROS overproduction of NOX2 and nitrotyrosine, reversed the reduction of anti-oxidant marker, SOD-1 as well as restored NO bioavailability in both HUVECs challenged with HG and in db/db diabetic mice. Similarly, HG-induced elevation of oxidative stress in HUVECs were reversed by SA, glibenclamide, and apocynin. This attests that HBN restores endothelial function and protects endothelial cells against oxidative damage induced by HG in HUVECs, isolated mouse aorta, and db/ db diabetic mice via modulating ROS mechanism, which subsequently increases NO bioavailability. This result demonstrates the potential role of HBN in preserving endothelial function and management of micro-or macrovascular complications in diabetes.

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Section: Discussionsupporting

confidence: 93%

Edible Bird’s Nest Protects Against Hyperglycemia-Induced Oxidative Stress and Endothelial Dysfunction

et al. 2020

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“…Cell viability between EC extract- and control-treated cells did not differ significantly. Seaweed extracts have been previously reported to be non-toxic to normal cells [ 47 ] and human keratinocyte (HaCaT) cells [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

“…The EC extract has shown antioxidant, anti-inflammation, and anti-cancer properties [ 26 , 27 , 59 , 60 ], and has previously been demonstrated to slow tumor cell growth rate [ 27 ], promote wound healing [ 28 ], and upregulate cancer cell apoptosis [ 29 ]. Moreover, extracts of this seaweed improved cardiovascular, liver, and metabolic parameters in obese rat models [ 30 ] and presented anti-diabetic effects in streptozotocin-induced type 2 diabetic mice [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…Eucheuma cottonii is a red seaweed previously reported to demonstrate antioxidant, anticoagulant, anti-tumor, and anti-inflammation properties [ 20 – 24 ]. Eucheuma cottonii is also known as Kappaphycus alvarezii (KA) or the “sea-bird nest” [ 25 , 26 ]. In addition, studies have reported that extracts produced from this seaweed can slow tumor cell growth rate [ 27 ], promote wound healing [ 28 ], and upregulate cancer cell apoptosis [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

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Dietary polysaccharide-rich extract from Eucheuma cottonii modulates the inflammatory response and suppresses colonic injury on dextran sulfate sodium-induced colitis in mice

Sudirman

Hsu

et al. 2018

PLoS ONE

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Inflammatory bowel disease (IBD) is a known medical burden in most developed countries and a significant cause of morbidity. The IBD label includes Crohn’s disease (CD) and ulcerative colitis (UC). Pharmacological and surgical intervention are the two main management approaches for IBD. Some drugs have been developed for IBD therapy, but accessibility is limited due to high costs. Furthermore, these agents have demonstrated inactivity over long-term treatment courses. Therefore, an urgent need is present for new treatment options that are safe, able to sustain clinical remission, and improve mucosal gut healing. Seaweed has received much attention in the pharmacological field owing to its various biomedical properties, including the prolongation of blood clotting time, as well as antitumor, anti-inflammation, and antioxidant effects. This study therefore aimed to examine the effects of a dietary polysaccharide-rich extract obtained from Eucheuma cottonii (EC) on a model of colitis. Colitis was induced in male BALB/c mice by the administration of 2.5% (w/v) dextran sulfate sodium (DSS) for 7 days. DSS-induced mice were treated with either one of three different doses of EC extracts (0.35, 0.70, and 1.75 g/kg body weight) or curcumin as a positive control (0.10 g/kg). Mice were sacrificed post-treatment and blood samples were collected. The disease activity index (DAI) and inflammatory cytokine levels (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-10) were measured. After treatment for 7 days, EC extract administration protected against weight loss and decreased the colon weight per length ratio. EC extract administration also decreased pro-inflammatory cytokine expression, increased IL-10 levels, and reduced colonic damage. Therefore, a dietary polysaccharide-rich extract from E. cottonii reduced DSS-induced bowel inflammation, thereby becoming a promising candidate for the treatment of colitis.

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“…In this procedure, 2,2-diphenyl-1-1-picrylhydrazyl (DPPH) assay was performed based on procedure proposed by the previous work [25]. The stock solution of 1 M DPPH was prepared in methanol and kept at -20°C until analysis.…”

Section: Dpph Free Radical Scavengingmentioning

confidence: 99%

Evaluation of protein content and antioxidant activity of edible bird’s nest by various methods

2020

Biointerface Res Appl Chem

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The aim of this study was to evaluate the protein content and antioxidant activity of instant and raw Edible bird’s nest (EBN). This study was conducted using two types of EBNs, which are instant and raw samples. All EBN samples were extracted via three types of extraction method, namely, salt, alkaline, and hot extractions. Lowry’s method was used to analyze protein content and the antioxidant activities were analyzed via free radical scavenging assay 1,1-diphenyl-2-pycrylhydrazyl (DPPH), total phenolic content (TPC) assay and ferric reducing antioxidant power (FRAP). This study showed that the highest protein was 2165.90 µg/mL obtained from raw EBN extracted via alkaline solution. In addition, it was also found that the protein concentration of raw EBN was higher compared to instant EBN for all types of extraction procedures. Results from antioxidant assay showed that there was no significant difference between DPPH and FRAP of both EBN. Moreover, TPC results showed that the there was no phenolic compounds detected via all extraction procedures.

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Antioxidant Activity of the Sea Bird Nest (Eucheuma Cottonii) and Its Radical Scavenging Effect on Human Keratinocytes

Cited by 9 publications

References 25 publications

Edible Bird’s Nest Protects Against Hyperglycemia-Induced Oxidative Stress and Endothelial Dysfunction

Edible Bird’s Nest Protects Against Hyperglycemia-Induced Oxidative Stress and Endothelial Dysfunction

Dietary polysaccharide-rich extract from Eucheuma cottonii modulates the inflammatory response and suppresses colonic injury on dextran sulfate sodium-induced colitis in mice

Evaluation of protein content and antioxidant activity of edible bird’s nest by various methods

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