Antioxidant Activity of the Isoflavonoids from the Roots of <i>Maackia amurensis</i>

Кулеш, Н. И.; Fedoreyev, S. A.; Веселова, М. В.; Mischenko, Natalia P.; Денисенко, В. А.; Dmitrenok, Pavel S.; Zverev, Yakov F.; Zamyatina, S. V.

doi:10.1177/1934578x1300800511

Cited by 4 publications

(9 citation statements)

References 10 publications

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“…Additionally, the Oglycoside linkage in structures was confirmed, depending on the characteristic down-field location of C-6" at δ 70.37 ppm. Also the presence of doublet at δ 5.39 for the anomeric proton H-1′′′ of the terminal sugar moiety was intrinsic for (1′′′ -6′′) O-glycoside moiety 28 . The assignment of all other 13 C NMR resonances of compound 4 was achieved by comparison with the corresponding data of structural related compounds 9,15,19,22,23 .…”

Section: Characterization and Identification Of Isolated Compoundsmentioning

confidence: 99%

Investigation of Livistona decipiens Leaf Methanol Extract and Evaluation of Antioxidant, Antimicrobial and Cytotoxic Activities

Ibrahim

Elshaarawy

Haggag

2018

Journal of Advanced Pharmacy Research

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Objectives: This study aimed to isolate the polyphenolic constituents from the methanol extract of Livistona decipiens Becc leaves and evaluate the antioxidant, cytotoxic and antimicrobial activities of the total methanol extract and ethyl acetate fraction. Methods: The ethyl acetate and n-butanol fractions of Livistona decipiens leaf methanol extract were subjected separately to different chromatographic separation techniques. Structures of the isolated compounds were established by different spectroscopic techniques (1 H / 13 C NMR). Antioxidant activity was evaluated by DPPH assay, while evaluation of cytotoxicity was done according to MTT cell viability assay. Antimicrobial activity was done by agar diffusion method. Results: seven compounds were isolated from the ethyl acetate and n-butanol fractions, five compounds were identified for the first time from this plant as apigenin-8-C-β-D-glucopyranoside (Vitexin) (1), Quercetin-6-C-β-D-glucopyranoside (2),apigenin-6,8-di-C-β-D-glucopyranoside (Vicenin II) (3), 6-O-methyl Kaempferol 3-O-glucopyranoside (4), luteolin-3-C-gentiobiosyl (5), while two compounds vis; luteolin-6-C-β-Dglucopyranoside (Isoorientin) (6) and luteolin-8-C-β-D-glucopyranoside (Orientin) (7), were isolated for the second time from this species. The ethyl acetate fraction has shown moderate activity against Gram positive and Gram negatvie bacteria, also it showed moderate antioxidant activity with IC50 = 23 ± 0.74 µg/ml when compared to ascorbic acid IC50 = 14.2 ± 0.35 µg/ml. Also ethyl acetate extract has shown cytotoxic activity on MCF-7 cells (human breast cancer cell line), HepG-2 (human hepatocellular carcinoma) and HeLa cells (human cervical cancer cell line), whereas, the methanol extract has shown lower activity. Conclusion: Livistona decipiens have potential medicinal value being rich in polyphenolics and being antioxidant, cytotoxic and antimicrobial drug.

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Section: Characterization and Identification Of Isolated Compoundsmentioning

confidence: 99%

Investigation of Livistona decipiens Leaf Methanol Extract and Evaluation of Antioxidant, Antimicrobial and Cytotoxic Activities

Ibrahim

Elshaarawy

Haggag

2018

Journal of Advanced Pharmacy Research

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“…Äëÿ áîëåå ýôôåêòèâíîãî ïðèìåíåíèÿ óíèêàëüíîãî ðåëèêòîâîãî ðàñòåíèÿ â ôàðìàöåâòè÷åñêîé ïðîìûøëåííîñòè âàaeíî áûëî îöåíèòü âîçìîaeíîñòü èñïîëüçîâàíèÿ äðóãèõ îðãàíîâ ðàñòåíèÿ, íàïðèìåð êîðíåé, â êà÷åñòâå àëüòåðíàòèâíîãî èñòî÷íèêà ñûðüÿ äëÿ ñîçäàíèÿ ôàðìàöåâòè÷åñêèõ ïðåïàðàòîâ. Íåäàâíî ìû ïîêàçàëè, ÷òî êîðíè, â îòëè÷èå îò äðåâåñèíû ðàñòåíèÿ M. amurensis, ñîäåðaeàò ñâûøå 78 % ãëèêîçèäíûõ ôîðì èçîôëàâîíîâ è ïòåðîêàðïàíîâ [12,13]. Ïðè ýòîì êîìïëåêñ èçîôëàâîíîèäîâ èç êîðíåé M. amurensis îáëàäàåò âûðàaeåííûìè àíòèîêñèäàíòíûìè [13] è ãåïàòîïðîòåêòèâíûìè ñâîéñòâàìè [12 -15] è ÿâëÿåòñÿ ïåðñïåêòèâíûì äëÿ ñîçäàíèÿ íîâûõ ôàðìàöåâòè÷åñêèõ ñðåäñòâ íà åãî îñíîâå.…”

Section: ðàñòåíèå ìààêèÿ àìóðñêàÿ (Maackia Amurensisunclassified

“…Íåäàâíî ìû ïîêàçàëè, ÷òî êîðíè, â îòëè÷èå îò äðåâåñèíû ðàñòåíèÿ M. amurensis, ñîäåðaeàò ñâûøå 78 % ãëèêîçèäíûõ ôîðì èçîôëàâîíîâ è ïòåðîêàðïàíîâ [12,13]. Ïðè ýòîì êîìïëåêñ èçîôëàâîíîèäîâ èç êîðíåé M. amurensis îáëàäàåò âûðàaeåííûìè àíòèîêñèäàíòíûìè [13] è ãåïàòîïðîòåêòèâíûìè ñâîéñòâàìè [12 -15] è ÿâëÿåòñÿ ïåðñïåêòèâíûì äëÿ ñîçäàíèÿ íîâûõ ôàðìàöåâòè÷åñêèõ ñðåäñòâ íà åãî îñíîâå. Öåëüþ íàñòîÿùåé ðàáîòû ÿâèëîñü äåòàëüíîå èññëåäîâàíèå õèìè÷åñêîãî ñîñòàâà âûäåëåííîãî èç ýêñòðàêòà êîðíåé ìààêèè àìóðñêîé êîìïëåêñà èçîôëàâîíîèäîâ è èçó÷åíèå åãî âëèÿíèÿ íà ñîñòîÿíèå óãëåâîäíîãî îáìåíà ïå÷åíè êðûñ â óñëîâèÿõ èíòîêñèêàöèè ÷åòûðåõõëîðèñòûì óãëåðîäîì.…”

Section: ðàñòåíèå ìààêèÿ àìóðñêàÿ (Maackia Amurensisunclassified

“…Ïîñëå äîïîëíèòåëüíîé î÷èñòêè ïîëó÷åííûõ ôðàêöèé íà êîëîíêå, çàïîëíåííîé ñîðáåíòîì YMC-GEL ODS-A (cèëèêàãåëü Ñ-18) â ñèñòåìå ðàñòâîðèòåëåé âîäà -ñïèðò ñ ãðàäèåíòíûì óâåëè÷åíèåì ñîäåðaeàíèÿ ñïèðòà â ñèñòåìå äî 40 %, ïîëó÷åíû ñîåäèíåíèÿ I -VII, X -XII ñ âûõîäàìè 3, Ïðåäâàðèòåëüíî îñâîáîaeäåííûé îò îñòàòî÷íûõ ðàñòâîðèòåëåé ñïèðòà, ãåêñàíà è î÷èùåííûé îò ðàñòèòåëüíûõ âîñêîâ è ëèïèäîâ ýêñòðàêò èç êîðíåé M. amurensis è ëåãàëîí aeèâîòíûì ââîäèëè âíóòðèaeåëó-äî÷íî ÷åðåç çîíä â äîçå 100 ìã/êã â âèäå âçâåñè â 1 % êðàõìàëüíîì êëåéñòåðå. Ïðè îïðåäåëåíèè êîëè÷åñòâåííîãî ñîñòàâà ýêñòðàêòà ñðåäè áèîëîãè÷åñêè àêòèâíûõ ôðàêöèé äîìèíèðóþùèìè áûëè èçîôëàâîíîèäû, ïîýòîìó ñòàíäàðòèçàöèþ ýêñòðàêòà èç ìààêèè ïðîâîäèëè ïî ñóììàðíîìó ñîäåðaeàíèþ èçîôëàâîíîèäîâ I -XII [13] è äîçó ââîäèìîãî ïðåïàðàòà ðàññ÷èòûâàëè â ìã èçîôëàâîíîèäîâ íà 1 êã ìàññû aeèâîòíîãî. Ñóììàðíîå ñîäåðaeàíèå èçîôëàâîíîèäîâ ñîñòàâëÿëî 77 ìã â 100 ìã ýêñòðàêòà êîðíåé M. amurensis.…”

Section: ðàñòåíèå ìààêèÿ àìóðñêàÿ (Maackia Amurensisunclassified

“…Èõ ñòðóêòóðû îïðåäåëåíû ìåòîäàìè õðîìàòîìàññ-ñïåêòîìåòðèè (òàáë. 1) è ßÌÐ-ñïåêòðîñêîïèè êàê 7-O-ãåíòèîáèîçèäû äàèäçåè-íà (I), ãåíèñòåèíà (III), àôðîìîçèíà (IV), ïñåâäîáàïòèãåíèíà (VI), ôîðìîíîíåòèíà (VII), 5-O-ìåòèëãåíèñòåèíà (XII), 3-O-ãåíòèîáîçèäû ìààêèàèíà (X) è ìåäèêàðïèíà (XI), äàèäçèí (II) è ãåíèñòèí (V)[13,16]. Êðîìå ýòîãî èç êîðíåé ýòîãî ðàñòåíèÿ âûäåëåíû 7-O-ïðèìâåðîçèäû ïñåâäîáàïòèãåíèíà (VIII) è ôîðìîíîíåòèíà (IX) (ðèñ.…”

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Влияние изофлавоноидов из корней <i>Maackia amurensis</i> на метаболические реакции печени при экспериментальном токсическом гепатите

Кулеш¹,

Fedoreyev²,

Веселова³

et al. 2016

Хим.-фарм. ж.

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Из спиртового экстракта корней дальневосточного реликтового растения маакии амурской (Maackia amurensis Ruper et Maxim.) выделен комплекс изофлавоноидов, содержащий свыше 78 % гликозидных форм изофлавонов и птерокарпанов. В состав этого комплекса входят 7-O-гентиобиозиды даидзеина, генистеина, афромозина, псевдобаптигенина, формононетина, 5-O-метилгенистеина, 3-O-гентиобозиды маакиаина и медикарпина, даидзин, генистин, 7-O-примверозилформононетин и новый 7-O-примверозилпсевдобаптигенин. На модели токсического гепатита, вызванного интоксикацией крыс четыреххлористым углеродом, исследовано влияние комплекса изофлавоноидов на состояние углеводного обмена печени крыс. Показано, что введение этого комплекса при CCl4-гепатите способствует снижению активности маркерных ферментов цитолиза и удельной массы печени, обеспечивает сохранение уровня глюкозы крови и окисленных никотинамидных коферментов (НАД+), нормализует содержание пирувата и лактата в печени животных. Действие комплекса изофлавоноидов, выделенного из маккии амурской, оказалось более эффективным в восстановлении реакций углеводного обмена печени (глюконеогенез, цикл Кребса), чем у эталонного гепатопротектора «Легалон®».

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Cytotoxic Prenylated Polyphenolic Compounds from Maackia amurensis Root Bark

Веселова

Fedoreyev

Tarbeeva

et al. 2017

Natural Product Communications

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Two new prenylated flavanones, isomaackiaflavanone A (1), isomaackiaflavanone B (2) and a new prenylated stilbene maackiastilbene (3), along with five known flavanones were isolated from the root bark of Maackia amurensis using repeated column chromatography on silica gel, Sephadex LH-20 and octadecyl silica (ODS-A) sorbents. The structures of the new compounds were elucidated by HPLC-PDA-MS, HR-ESI-MS, 1 H, 13 C, 1 H-1 H COSY, HSQC, ROESY and HMBC NMR analyses. The cytotoxicity of compounds 1-2, 4-8 against two human cancer cell lines HeLa and SK-MEL-5 was tested using the MTS method. Compounds 4, 6 and 8 showed the strongest cytotoxic activity among the compounds tested with IC 50 values of 6.5, 8.8 and 7.7 μM, against SK-MEL-5 cells and 8.2, 18.8 and 12 μM against HeLa cells, respectively.

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Antioxidant Activity of the Isoflavonoids from the Roots of Maackia amurensis

Cited by 4 publications

References 10 publications

Investigation of Livistona decipiens Leaf Methanol Extract and Evaluation of Antioxidant, Antimicrobial and Cytotoxic Activities

Investigation of Livistona decipiens Leaf Methanol Extract and Evaluation of Antioxidant, Antimicrobial and Cytotoxic Activities

Влияние изофлавоноидов из корней <i>Maackia amurensis</i> на метаболические реакции печени при экспериментальном токсическом гепатите

Cytotoxic Prenylated Polyphenolic Compounds from Maackia amurensis Root Bark

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