Antioxidant Activity of Supramolecular Water-Soluble Fullerenes Evaluated by β-Carotene Bleaching Assay

Takada, Hiroya; Kokubo, Ken; Matsubayashi, Kenji; Oshima, Tairo

doi:10.1271/bbb.60491

Cited by 57 publications

(31 citation statements)

References 39 publications

(50 reference statements)

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“…That is to say the antioxidant activities are not comparable when different assay systems are used. 26,27) These results also indicate that the antioxidant properties of these essential oils are distinct. In the NO radical scavenging assay, the essential oil of T. vulgaris exhibited the lowest IC 50 value.…”

Section: Resultsmentioning

confidence: 73%

Antimicrobial, Antioxidant, and Anti-Inflammatory Activities of Essential Oils from Five Selected Herbs

Tsai

Lin

et al. 2011

Bioscience, Biotechnology, and Biochemistry

154

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Section: Resultsmentioning

confidence: 73%

Antimicrobial, Antioxidant, and Anti-Inflammatory Activities of Essential Oils from Five Selected Herbs

Tsai

Lin

et al. 2011

Bioscience, Biotechnology, and Biochemistry

154

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“…4 it can be seen that C 60 -PVP, in the same concentration range, suppresses lipid peroxidation similarly to BHT. Takada et al reported significant inhibitory effect of PVP entrapped fullerene on the oxidative discoloration of ␤-carotene [23], while Xiao et al have shown that the same supramolecular structure diminished the ROS amounts in the terms of the molecu- Fullerene coated with C 12 E 10 and TX100 demonstrated high antioxidative efficacy, decreasing the TBARS for more than 50% related to control at concentration of 8.60 and 8.14 M, respectively.…”

Section: Antioxidant Capacity Of C 60 Micellar Solutions (Tbars Assay)mentioning

confidence: 99%

“…These findings open the possibility for transferring individual molecules of fullerene to biological cells via liposome interaction with cell membrane. Other macromolecules such as calixarene [21], polyvinylpyrrolidone (PVP) [22][23][24], lecithin [25] have been used to prepare water stable C 60 aggregates. Surfactant solutions also offer a versatile solubilization method, since the micellar environment is chemically inert towards fullerenes.…”

Section: Introductionmentioning

confidence: 99%

Solubilization of fullerene C60 in micellar solutions of different solubilizers

Torres

Poša

Srdjenović

et al. 2011

Colloids and Surfaces B: Biointerfaces

108

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a b s t r a c tFullerene (C 60 ), the third carbon allotrope, is a classical engineered material with the potential application in biomedicine. However, extremely high hydrophobicity of fullerene hampers its direct biomedical evaluation and application. In this work, we investigated the solubilization of fullerene using 9 different solubility enhancers: Tween 20, Tween 60, Tween 80, Triton X-100, PVP, polyoxyethylene (10) lauryl ether, n-dodecyl trimethylammonium chloride, myristyl trimethylammonium bromide and sodium dodecyl sulphate and evaluated its antioxidant activity in biorelevant media. The presence of C 60 entrapped in surfactant micelles was confirmed by UV/VIS spectrometry. The efficacy of each modifier was evaluated by chemometric analysis using experimental data for investigating the relationship between solubilization and particle size distribution. Hierarchical clustering and principal component analysis was applied and showed that non-ionic surfactants provide better solubilization efficacy (>85%). A correlation was established (r = 0.975) between the degree of solubilization and the surfactant structure. This correlation may be used for prediction of C 60 solubilization with non-tested solubility modifiers. Since the main potential biomedical applications of fullerene are based on its free radical quenching ability, we tested the antioxidant potential of fullerene micellar solutions. Lipid peroxidation tests showed that the micellar solutions of fullerene with Triton and polyoxyethylene lauryl ether kept high radical scavenging activity, comparable to that of aqueous suspension of fullerene and BHT. The results of this work provide a platform for further solubilization and testing of pristine fullerene and its hydrophobic derivatives in a biological benign environment.

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“…This peroxyl radical reacts with β-Carotene to form a stable β-Carotene radical and subsequently, the amount of β-Carotene reduces in testing solution. If an antioxidant is present in the testing solution, it reacts competitively with the peroxyl radical (Takada et al 2006). Therefore antioxidant effects are easily monitored by bleaching the colour of a test solution with a spectrophotometer at 470 nm, which is the typical absorbance of β-Carotene.…”

Section: Changes In Tba Valuementioning

confidence: 99%

In vitro antioxidant assay of medium chain fatty acid rich rice bran oil in comparison to native rice bran oil

2014

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Antioxidant Activity of Supramolecular Water-Soluble Fullerenes Evaluated by β-Carotene Bleaching Assay

Cited by 57 publications

References 39 publications

Antimicrobial, Antioxidant, and Anti-Inflammatory Activities of Essential Oils from Five Selected Herbs

Antimicrobial, Antioxidant, and Anti-Inflammatory Activities of Essential Oils from Five Selected Herbs

Solubilization of fullerene C60 in micellar solutions of different solubilizers

In vitro antioxidant assay of medium chain fatty acid rich rice bran oil in comparison to native rice bran oil

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