Antioxidant activity of polysaccharide purified from Acanthopanax koreanum Nakai stems in vitro and in vivo zebrafish model

Kang, Min‐Cheol; Kim, Seo-Young; Kim, Eun-A.; Lee, Ji-Hyeok; Kim, Young-Sun; Yu, Seok-Kyu; Chae, Jun Byoung; Choe, In-Hu; Cho, Jin Hun; Jeon, You‐Jin

doi:10.1016/j.carbpol.2015.02.043

Cited by 69 publications

(31 citation statements)

References 40 publications

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“…No reduction in survival rate or morphological abnormalities were observed, demonstrating that these molecules, even at the higher exposure concentrations used, did not have any harmful effects, not only on human cells, but also on zebrafish development. This was in agreement with other publications that demonstrated the absence of toxicity of EPS on human dermal fibroblasts [64] and on zebrafish embryos [47]. Furthermore, the exposure of zebrafish embryos and larvae to the Phormidium sp.…”

Section: Discussionsupporting

confidence: 93%

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Anti-Inflammatory Activity of Exopolysaccharides from Phormidium sp. ETS05, the Most Abundant Cyanobacterium of the Therapeutic Euganean Thermal Muds, Using the Zebrafish Model

et al. 2020

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The Euganean Thermal District (Italy) represents the oldest and largest thermal center in Europe, and its therapeutic mud is considered a unique product whose beneficial effects have been documented since Ancient Roman times. Mud properties depend on the heat and electrolytes of the thermal water, as well as on the bioactive molecules produced by its biotic component, mainly represented by cyanobacteria. The investigation of the healing effects of compounds produced by the Euganean cyanobacteria represents an important goal for scientific validation of Euganean mud therapies and for the discovering of new health beneficial biomolecules. In this work, we evaluated the therapeutic potential of exopolysaccharides (EPS) produced by Phormidium sp. ETS05, the most abundant cyanobacterium of the Euganean mud. Specifically, Phormidium EPS resulted in exerting anti-inflammatory and pro-resolution activities in chemical and injury-induced zebrafish inflammation models as demonstrated using specific transgenic zebrafish lines and morphometric and expression analyses. Moreover, in vivo and in vitro tests showed no toxicity at all for the EPS concentrations tested. The results suggest that these EPS, with their combined anti-inflammatory and pro-resolution activities, could be one of the most important therapeutic molecules present in the Euganean mud and confirm the potential of these treatments for chronic inflammatory disease recovery.

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Section: Discussionsupporting

confidence: 93%

“…Based on these results and literature data on the use of EPS extracted from other species [47,48], Phormidium EPS were used at a 50 µg/mL concentration.…”

Section: Phormidium Eps Treatment Has No Toxicological or Teratogenicmentioning

confidence: 99%

Anti-Inflammatory Activity of Exopolysaccharides from Phormidium sp. ETS05, the Most Abundant Cyanobacterium of the Therapeutic Euganean Thermal Muds, Using the Zebrafish Model

et al. 2020

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“…To analyze neutral sugars CPs were hydrolyzed using 4 M of trifluoroacetic acid in sealed glass tubes. Analysis was carried out using the method described by Kang et al (2015).…”

Section: Characterization Of Cps By Fourier Transform Infrared Spectrmentioning

confidence: 99%

FTIR characterization and antioxidant activity of water soluble crude polysaccharides of Sri Lankan marine algae

Fernando

Sanjeewa

Samarakoon

et al. 2017

ALGAE

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178

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Polysaccharides of marine algae exhibit different structural characteristics and interesting biological functions. In this study, crude polysaccharides (CP) of eleven Sri Lankan marine algae obtained through hot water extraction and ethanol precipitation were investigated for DPPH, alkyl, and hydroxyl radical scavenging activities using electron spin resonance spectrometry and for intracellular reactive oxygen species scavenging activity in the Chang liver cell line. Characterization of CPs was done by Fourier transform infrared (FTIR) spectroscopy and by analysis of the monosaccharide composition. Time-dependent density functional theory quantum-chemical calculations at the RB3LYP/6-31G(d,p) level for constructed dimeric units of the corresponding polysaccharides were used to resolve the FTIR spectra. CPs from Chnoospora minima showed the highest DPPH and alkyl radical scavenging activities and higher intracellular reactive oxygen species scavenging effects for both AAPH and H 2 O 2 induced ROS production in "Chang" cells. The major polysaccharide constituent in C. minima CP was identified as fucoidan and it displayed a higher sulfate content. The degree of sulfation of these polysaccharides suggests a positive correlation with the observed antioxidant properties.

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“…Plant polysaccharides are important phytochemicals that occur in neutral and acidic types (Leivas and others ; Yi and others ). Polysaccharides can be effectively used to enhance immunity (Yu and others ) and to elicit antioxidation (Kang and others ), antitumor (Liu and others ; Pei and others ) and antifatigue (Zheng and others ) properties. For example, the antitumor effects of Panax ginseng polysaccharides (Fu and others ; Sun and others ) and the cardiovascular effects of Salvia miltiorrhiza polysaccharides (Geng and others ) had been described.…”

Section: Introductionmentioning

confidence: 99%

Structural Characterization and Antifatigue Effect In Vivo of Maca (Lepidium meyenii Walp) Polysaccharide

Tang¹,

Jin²,

Xie³

et al. 2017

Journal of Food Science

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Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo. The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses. The antifatigue effect of MP was evaluated by using a mouse weight-loaded swimming model. MP is an acidic heteropolysaccharide with an average molecular weight (M ) of 793.5 kDa. It is composed of D-GalA: D-Glc: L-Ara: D-Man: D-Gal: L-Rha = 35.07:29.98:16.98:13.01:4.21:0.75 (mol, %). The findings revealed that MP contained β-1,3-Galp(A), β-1,3-Glcp, and α-1, 3-Manp linked alternatingly to form a backbone (5:4:1). MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice. Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01). High-dosage MP could significantly reduce the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05). MP is an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca.

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Antioxidant activity of polysaccharide purified from Acanthopanax koreanum Nakai stems in vitro and in vivo zebrafish model

Cited by 69 publications

References 40 publications

Anti-Inflammatory Activity of Exopolysaccharides from Phormidium sp. ETS05, the Most Abundant Cyanobacterium of the Therapeutic Euganean Thermal Muds, Using the Zebrafish Model

Anti-Inflammatory Activity of Exopolysaccharides from Phormidium sp. ETS05, the Most Abundant Cyanobacterium of the Therapeutic Euganean Thermal Muds, Using the Zebrafish Model

FTIR characterization and antioxidant activity of water soluble crude polysaccharides of Sri Lankan marine algae

Structural Characterization and Antifatigue Effect In Vivo of Maca (Lepidium meyenii Walp) Polysaccharide

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