Antioxidant Activity of Pitcher Extracts from Three Nepenthes Species

Rosli, Muhammad Aqil Fitri; Azizan, Kamalrul Azlan; Goh, Hoe-Han

doi:10.17576/jsm-2018-4712-17

Cited by 8 publications

(7 citation statements)

References 27 publications

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“…These metabolites contain anti-microbial properties that prevent microbial competition for nutrient absorption. Efforts in the profiling of secondary metabolites from Nepenthes pitchers and their bioactivity are on-going ( Rosli et al, 2017 , 2018 ; Dávila-Lara et al, 2020 ). Meanwhile, genes involved in the biosynthesis of secondary metabolites such as phenylpropanoids, sesquiterpenoids, and triterpenoids in N. ampullaria were found to be influenced by endogenous protein depletion ( Goh et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Proteomic Analyses of Nepenthes ampullaria and Nepenthes rafflesiana Reveal Parental Molecular Expression in the Pitchers of Their Hybrid, Nepenthes × hookeriana

et al. 2021

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Nepenthes is a genus comprising carnivorous tropical pitcher plants that have evolved trapping organs at the tip of their leaves for nutrient acquisition from insect trapping. Recent studies have applied proteomics approaches to identify proteins in the pitcher fluids for better understanding the carnivory mechanism, but protein identification is hindered by limited species-specific transcriptomes for Nepenthes. In this study, the proteomics informed by transcriptomics (PIT) approach was utilized to identify and compare proteins in the pitcher fluids of Nepenthes ampullaria, Nepenthes rafflesiana, and their hybrid Nepenthes × hookeriana through PacBio isoform sequencing (Iso-Seq) and liquid chromatography-mass spectrometry (LC-MS) proteomic profiling. We generated full-length transcriptomes from all three species of 80,791 consensus isoforms with an average length of 1,692 bp as a reference for protein identification. The comparative analysis found that transcripts and proteins identified in the hybrid N. × hookeriana were more resembling N. rafflesiana, both of which are insectivorous compared with omnivorous N. ampullaria that can derive nutrients from leaf litters. Previously reported hydrolytic proteins were detected, including proteases, glucanases, chitinases, phosphatases, nucleases, peroxidases, lipid transfer protein, thaumatin-like protein, pathogenesis-related protein, and disease resistance proteins. Many new proteins with diverse predicted functions were also identified, such as amylase, invertase, catalase, kinases, ligases, synthases, esterases, transferases, transporters, and transcription factors. Despite the discovery of a few unique enzymes in N. ampullaria, we found no strong evidence of adaptive evolution to produce endogenous enzymes for the breakdown of leaf litter. A more complete picture of digestive fluid protein composition in this study provides important insights on the molecular physiology of pitchers and carnivory mechanism of Nepenthes species with distinct dietary habits.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic and Proteomic Analyses of Nepenthes ampullaria and Nepenthes rafflesiana Reveal Parental Molecular Expression in the Pitchers of Their Hybrid, Nepenthes × hookeriana

et al. 2021

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“…Formation of Ni‐NPs was evidenced by changing the color of nickel acetate (pale green) to black color. [ 33,34 ]…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Nanocapsules Based on Biosynthesized Nickel Nanoparticles and Potato Starch: Antimicrobial, Antioxidant, and Anticancer Activity

et al. 2021

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Biogenic fabrication of nanoparticles (NPs) by fungi becomes a promising protocol due to effective and environmentally friendly bioprocess. The capsulation of nanoparticles into biopolymer has received considerable attention owing to improve stability. The nickel nanoparticles (Ni-NPs) are synthesized using Penicillium chrysogenum and capsulated into potato starch to produce nanocapsules (Ni-NPs@st) for enhancing their antimicrobial, antioxidant, and anticancer properties. Physicochemical properties of Ni-NPs and Ni-NPs@st are carried out using UV-vis, FTIR, dynamic light scattering (DLS), TEM, and thermal gravimetric analysis (TGA). Results reveal that Ni-NPs@st has antimicrobial activity more than Ni-NPs against Escherichia coli ATCC25922, Bacillus subtilis ATCC605, Candida albicans ATCC90028, Cryptococcus neoformans ATCC 14116, Aspergillus niger RCMB 02724, and A. fumigatus RCMB 02568. Moderate antioxidant activity is recorded using Ni-NPs@st and Ni-NPs with IC50 60 and 97 µg mL −1 , respectively, in contrast to IC50 (7 µg mL −1 ) of ascorbic acid. Cytotoxicity test of Ni-NPs@st and Ni-NPs against Vero cell line indicated that IC50 of Ni-NPs@st and Ni-NPs is 204 and 238 µg mL −1 , respectively. Likewise, moderate anticancer activity of Ni-NPs@st and Ni-NPs toward cancerous cell lines MCF7 is observed with IC50 118 and 217 µg mL −1 , respectively. This study shows prospective biological activities for Ni-NPs@st nanocapsule which can be treat the chronic infections.

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“…Therefore, it is indicative that peristome development is activated upon pitcher opening. Meanwhile, secondary metabolism involving phenylpropanoids and terpenoids might confer a protective effect against oxidative stress from cellular metabolism 44 . Many secondary metabolites also have antifeedant and antifungal functions, especially flavonoids and naphthoquinones 45 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptome-wide shift from photosynthesis and energy metabolism upon endogenous fluid protein depletion in young Nepenthes ampullaria pitchers

Goh

Baharin

Salleh

et al. 2020

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faris 'imadi Mohd Salleh, Rishiesvari Ravee, Wan nor Adibah Wan Zakaria & normah Mohd noor carnivorous pitcher plants produce specialised pitcher organs containing secretory glands, which secrete acidic fluids with hydrolytic enzymes for prey digestion and nutrient absorption. The content of pitcher fluids has been the focus of many fluid protein profiling studies. These studies suggest an evolutionary convergence of a conserved group of similar enzymes in diverse families of pitcher plants. A recent study showed that endogenous proteins were replenished in the pitcher fluid, which indicates a feedback mechanism in protein secretion. This poses an interesting question on the physiological effect of plant protein loss. However, there is no study to date that describes the pitcher response to endogenous protein depletion. To address this gap of knowledge, we previously performed a comparative RNA-sequencing experiment of newly opened pitchers (D0) against pitchers after 3 days of opening (D3C) and pitchers with filtered endogenous proteins (>10 kDa) upon pitcher opening (D3L). Nepenthes ampullaria was chosen as a model study species due to their abundance and unique feeding behaviour on leaf litters. The analysis of unigenes with top 1% abundance found protein translation and stress response to be overrepresented in D0, compared to cell wall related, transport, and signalling for D3L. Differentially expressed gene (DEG) analysis identified DEGs with functional enrichment in protein regulation, secondary metabolism, intracellular trafficking, secretion, and vesicular transport. the transcriptomic landscape of the pitcher dramatically shifted towards intracellular transport and defence response at the expense of energy metabolism and photosynthesis upon endogenous protein depletion. This is supported by secretome, transportome, and transcription factor analysis with RT-qPCR validation based on independent samples. This study provides the first glimpse into the molecular responses of pitchers to protein loss with implications to future cost/benefit analysis of carnivorous pitcher plant energetics and resource allocation for adaptation in stochastic environments.Carnivorous plants are commonly found in habitats deprived of nutrients, especially nitrogen and phosphorus. To qualify as carnivorous, a plant must have at least one adaptation for active attraction, capture, digestion, and clear nutritional benefit from carnivory 1 . The convergent evolution of passive pitfall traps resulted in carnivorous pitcher plants of diverse orders and families, including Nepenthaceae (Caryophyllales), Cephalotaceae (Oxalidales), and Sarraceniaceae (Ericales) at distant geographical locations but sharing similar digestive fluid proteins 2-4 . A species-rich monotypic group of tropical pitcher plants from genus Nepenthes develop attractive pitchers of diverse shapes and sizes 5 . Recently, two highly conserved leaf developmental regulatory genes, ASYMMETRIC LEAVES1 (AS1) and REVOLUTA (REV), have been shown to be key in pitcher developme...

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Antioxidant Activity of Pitcher Extracts from Three Nepenthes Species

Cited by 8 publications

References 27 publications

Transcriptomic and Proteomic Analyses of Nepenthes ampullaria and Nepenthes rafflesiana Reveal Parental Molecular Expression in the Pitchers of Their Hybrid, Nepenthes × hookeriana

Transcriptomic and Proteomic Analyses of Nepenthes ampullaria and Nepenthes rafflesiana Reveal Parental Molecular Expression in the Pitchers of Their Hybrid, Nepenthes × hookeriana

Synthesis of Nanocapsules Based on Biosynthesized Nickel Nanoparticles and Potato Starch: Antimicrobial, Antioxidant, and Anticancer Activity

Transcriptome-wide shift from photosynthesis and energy metabolism upon endogenous fluid protein depletion in young Nepenthes ampullaria pitchers

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