Antioxidant activity of Macedonian Juniper ( Juniperus communis L.) fruit extracts

Kurti, Leonard; Jovanova, Blagica; Kelmendi, Ariana; Hamidi, Mojtaba; Kadifkova-Panovska, Tatjana; Kulevanova, S.

doi:10.1016/j.toxlet.2015.08.296

Cited by 2 publications

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“…The evaluation of the extraction efficiency and the phytochemical assays offers a first glance on the extract composition. The polyphenolics content it’s comparable with literature data regarding European native juniper extracts (Kurti et al [ 24 ] reported values ranging from 4.7 to 5.83 mg g −1 dry plant weight for ethanol extracts obtained from 20 localities in the Republic of Macedonia, while Miceli et al [ 45 ] reported values of 17 to about 60 mg GAE g −1 extract for methanol extracts of Juniperus communis L. var. communis and Juniperus communis L. var.…”

Section: Discussionsupporting

confidence: 80%

Genoprotective, antioxidant, antifungal and anti-inflammatory evaluation of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills

Fierăscu

Ungureanu

Avramescu

et al. 2018

BMC Complement Altern Med

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BackgroundJuniperus communis L. represents a multi-purpose crop used in the pharmaceutical, food, and cosmetic industry. Several studies present the possible medicinal properties of different Juniperus taxa native to specific geographical area. The present study aims to evaluate the genoprotective, antioxidant, antifungal and anti-inflammatory potential of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills.MethodsThe prepared hydroethanolic extract of Juniperus communis L. was characterized by GC-MS, HPLC, UV-Vis spectrometry and phytochemical assays. The antioxidant potential was evaluated using the DPPH assay, the antifungal effect was studied on Aspergillus niger ATCC 15475 and Penicillium hirsutum ATCC 52323, while the genoprotective effect was evaluated using the Allium cepa assay. The anti-inflammatory effect was evaluated in two inflammation experimental models (dextran and kaolin) by plethysmometry. Male Wistar rats were treated by gavage with distilled water (negative control), the microemulsion (positive control), diclofenac sodium aqueous solution (reference) and microemulsions containing juniper extract (experimental group). The initial paw volume and the paw volumes at 1, 2, 3, 4, 5 and 24 h were measured.ResultsTotal terpenoids, phenolics and flavonoids were estimated to be 13.44 ± 0.14 mg linalool equivalent, 19.23 ± 1.32 mg gallic acid equivalent, and 5109.6 ± 21.47 mg rutin equivalent per 100 g of extract, respectively. GC-MS characterization of the juniper extract identified 57 volatile compounds in the sample, while the HPLC analysis revealed the presence of the selected compounds (α-pinene, chlorogenic acid, rutin, apigenin, quercitin). The antioxidant potential of the crude extract was found to be 81.63 ± 0.38% (measured by the DPPH method). The results of the antifungal activity assay (for Aspergillus niger and Penicillium hirsutum) were 21.6 mm, respectively 17.2 mm as inhibition zone. Test results demonstrated the genoprotective potential of J. communis undiluted extract, inhibiting the mitodepressive effect of ethanol. The anti-inflammatory action of the juniper extract, administered as microemulsion in acute-dextran model was increased when compared to kaolin subacute inflammation induced model.ConclusionThe hydroalcoholic extract obtained from wild-growing Juniperus communis native to Romanian southern sub-Carpathian hills has genoprotective, antioxidant, antifungal and anti-inflammatory properties.

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Section: Discussionsupporting

confidence: 80%

Genoprotective, antioxidant, antifungal and anti-inflammatory evaluation of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills

Fierăscu

Ungureanu

Avramescu

et al. 2018

BMC Complement Altern Med

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“…In traditional medicine they are used as diuretics, appetizers, carminatives, stomachics, anticonvulsants and antihypertensive agents, as well as in the treatment of headaches, fever, bronchitis, asthma and some gynecological disorders [5,6]. Literature data also indicate remarkable antioxidant, antimicrobial and hypoglycemic activities of these berries [7][8][9][10]. Furthermore, juniper extracts have found wide applications in pharmaceutical industry, perfumery and aromatherapy [11].…”

Section: Introductionmentioning

confidence: 99%

Bioactivity of Juniperus communis essential oil and post-distillation waste: Assessment of selective toxicity against food contaminants

Nikolić

Vasilijević

Ćirić

et al. 2019

ARCH BIOL SCI BELGRA

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Previously chemically characterized Juniperus communis essential oil (EO) and post-distillation waste (PDW) were tested for cytotoxicity and antimicrobial activity against food contaminants. Microdilution assay showed that PDW induced moderate antifungal (minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values, ranging between 0.118-0.900 mg mL-1), and an antibacterial effect against Listeria monocytogenes (MIC and minimum bactericidal concentration (MBC) were 0.39 and 0.74 mg mL-1 , respectively). Combinations of EO/PDW with selected antibiotics induced synergistic antilisterial activity in the checkerboard assay. The MTT assay determined that cytotoxicity against colon cancer cells was high for the EO but negligible for PDW (IC 50 values were 0.087-0.106 and 1.450-6.840 mg mL-1 , respectively). The selectivity indices indicated high selectivity of PDW against tested fungi and L. monocytogenes. In the adhesion-inhibition assay, PDW reduced in vitro adhesion of L. monocytogenes to colon cells (29-62% of inhibition). In conclusion, PDW exhibited an antimicrobial effect against important food spoilage and poisoning fungi and L. monocytogenes, and also reduced in vitro adhesion of L. monocytogenes to colon cells. The results indicate that J. communis PDW could be considered as natural preservative against food spoilage and poisonous fungi, and as an adjuvant to conventional therapy of listeriosis.

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Antioxidant activity of Macedonian Juniper ( Juniperus communis L.) fruit extracts

Cited by 2 publications

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Genoprotective, antioxidant, antifungal and anti-inflammatory evaluation of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills

Genoprotective, antioxidant, antifungal and anti-inflammatory evaluation of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills

Bioactivity of Juniperus communis essential oil and post-distillation waste: Assessment of selective toxicity against food contaminants

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