Antioxidant activity of Lactobacillus plantarum JM113 in vitro and its protective effect on broiler chickens challenged with deoxynivalenol1

Abstract

The aim of this experiment was to study the antioxidant capacity of JM113 isolated from healthy intestinal contents of Tibetan chicken and its protective effect on broiler chickens challenged with deoxynivalenol (DON). Compared with PZ01 and M23, JM113 demonstrated maximum reducing ( < 0.05) activity and resistance in the presence of 1.2 mmol/L hydrogen peroxide, and great scavenging ability ( < 0.05) against hydroxyl, superoxide anion, and 1,1-diphenyl-2-picrylhydrazyl radicals in vitro. For each strain, the antioxidant activities of live bacterial strains were greater ( < 0.05) than of cell free extracts and dead bacterial strains. To examine the antioxidant capacity of JM113 in vivo, 192 1-d-old Arbor Acres chicks were randomly divided into 4 treatments groups consisting of 6 replicates with 8 birds per replicate. The dietary treatments were 1) control; 2) control diet supplemented with JM113 at 1 × 10 cfu/kg; 3) control diet contaminated with DON at 10 mg/kg; 4) control diet contaminated with DON at 10 mg/kg and supplemented with JM113 at 1 × 10 cfu/kg. Dietary supplementation with DON decreased ( < 0.05) superoxide dismutase activity in serum and increased ( < 0.05) malondialdehyde in the jejunal mucosa of broilers, compared to the control. However, supplementation with JM113 to both the DON-contaminated diet and the control diet, caused a significant reduction ( < 0.05) in malondialdehyde activity in the jejunal mucosa. A reduction ( < 0.05) in expression of nuclear factor erythroid 2-related factor 2 was observed in the jejunal mucosa of broilers fed dietary supplementation with DON, whereas the mRNA levels of and its corresponding downstream gene increased ( < 0.05) with JM113 treatment. Addition of JM113 resulted in longer villi ( < 0.05), even in combination with DON compared to the DON group. JM113 treatment, especially in the DON plus JM113 group, up-regulated ( < 0.05) the expression of mRNA. In conclusion, the present study demonstrates that the JM113 strain has great antioxidant activity and supplementation in feed protected the integrity of the intestinal barrier in broilers challenged with DON, suggesting its use for alleviation of negative effects of DON in poultry.

Lactic Acid Bacteria as Antifungal and Anti‐Mycotoxigenic Agents: A Comprehensive Review

“…Exposure of CFS of two L. plantarum strains in combination with DON significantly prevented these toxic effects on intestinal mucosa layers in piglets. Apart from these studies, various effects of LAB strains in ameliorating DON‐related toxigenic effects have been reported in ex vivo studies conducted on animals (Wan, Turner, Allen, & El‐Nezami, ; Yang, Li, Duan, & Yang, ). In a recent study (Wu et al., ), DON‐related intestinal toxicity in broiler chickens was found to be related to changes in intestinal morphology, particular jejunum and duodenum, that included shortening of villi and deepening of crypts.…”

“…The use of LAB strains with antioxidative effects has proved to be an effective approach to combat with DON-caused oxidative stress. For instance, Yang, Li et al (2017) demonstrated the amelioration of DON-based oxidative intestinal damage of broiler chickens by L. plantarum strain JM113 with a well-characterized anti-oxidant activity. There are many LAB strains that have been reported to have antioxidant activity (Amaretti et al, 2013;Li et al, 2012) and thus such strains are ideal supplements for the alleviation of oxidative stress caused by DON and many other mycotoxins.…”

Intestinal toxicity of deoxynivalenol is limited by supplementation with Lactobacillus plantarum JM113 and consequentially altered gut microbiota in broiler chickens

“…In comparison, Lactobacillus is considered to be a good candidate since it can improve intestinal health by stimulating the immune system, modulating gut microbiota, and preventing oxidative damage [ 22 ], thereby eliminating the hazards of DON. The Lactobacillus plantarum ( L. plantarum ) JM113 strain has high antioxidant activity and thereby supplementation in feed has the potential to protect the integrity of the intestinal barrier in broilers challenged with DON [ 22 ]. This study was carried out to evaluate the beneficial effects of L. plantarum JM113 on the intestinal toxicity of DON in broilers.…”

Effects ofin ovofeeding of vitamin C on post-hatch performance, immune status and DNA methylation-related gene expression in broiler chickens

“…The plasma antioxidant system is an important part of a broiler's antioxidant system, which plays an important role in enhancing anti-stress ability (24) . And T-AOC, GSH-Px and superoxide dismutase activities and malondialdehyde content are usually used as indicators for measuring plasma antioxidant capacity (25,26) . To the best of our knowledge, there have been few studies on the effect of IOF of vitamin C on plasma antioxidant capacity of broilers.…”