Antioxidant Activity ofMyristica malabaricaExtracts and Their Constituents

Abstract

The 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay of the ether, methanol, and aqueous extracts of the spice Myristica malabarica (rampatri) revealed the methanol extract to possess the best antioxidant activity. Column chromatography of the methanol extract led to the isolation of a new 2-acylresorcinol and four known diarylnonanoids of which the diarylnonanoid, malabaricone C, showed the maximum DPPH scavenging activity. Malabaricone C could prevent both Fe(II)- and 2,2'-azobis(2-amidinopropane)dihydrochloride-induced lipid peroxidation (LPO) of rat liver mitochondria more efficiently than curcumin. The anti-LPO activity of malabaricone C was attributed to its better radical scavenging and Fe(II) chelation capacities. The superior activity of malabaricone C was rationalized by a systematic structure-activity correlation of the results obtained with the structurally related diarylnonanoids and curcumin. Malabaricone C also prevented the gamma-ray-induced damage of pBR322 plasmid DNA in a concentration-dependent manner. The radioprotective activity was found to correlate with its (*)OH radical scavenging property, which matched well with that of d-mannitol.

Biochemical Characterization of Tunisian Cichorium Intybus L. Roots and Optimization of Ultrasonic Inulin Extraction

“…The antiradical activity was finally expressed as IC50 (µg ml-1). A lower IC50 value corresponds to a higher antioxidant activity of the plant extract 19 . All samples were analyzed in three replications.…”

Isolation of some compounds from nutmeg and their antioxidant activities

“…Compound 2: 1 H-NMR (CDCl 3 -d 6 , 500 MHz) δ: 1.373 (3H, dd, J = 6.9 Hz, 2.4 Hz, H-9), 1.869 (3H, dd, J = 1.5 Hz, 6.6 Hz, H-9'), 3.447 (1H, dd, J = 9.4Hz, 6.6Hz, H-8), 3.876 (6H, s, -OCH 3 -3, -OCH 3 -3'), 5.099 (1H, d, J = 8.4Hz, H-7 Compound 3 (27 mg), white crystals (petroleum-acetone), showed [M] + peak at m/z = 342. Its structure was established by comparing NMR data with those reported for malabaricone B (Pham et al 2000;Patro et al 2005). This compound was previously isolated from mace but this is the first time it has been isolated from nutmeg.…”

“…Compound 4 (43 mg), light yellow crystals (petroleum-acetone), gave a similar NMR spectrum data as that obtained with compound 3. Its molecular formula was determined to be C 21 H 26 O 5 by EI-MS ([M] + at m/z 358) and was established as malabaricone C by comparing the NMR data with those reported (Pham et al 2000;Patro et al 2005). (21), 248 (39), 220 (17), 194 (13), 165 (27), 149 (14), 137 (100), 123 (72), 110 (78).…”

Antipromastigote activity of the malabaricones of Myristica malabarica (rampatri)

“…The F2 sub-fraction was subjected to rigorous column chromatography (silica gel, 0-20% EtOAc/hexane) to isolate five compounds 1-5, which were fully characterized using IR and 1 H NMR spectroscopic data. The yields reported for compounds 1-5 are with respect to the weight of dry fruit rinds (Patro et al, 2005). Compounds 2-5 that were used for the present investigation were identified as malabaricones A-D. Each of the test samples was dissolved in tissue culture grade dimethyl sulfoxide (DMSO) (20 mg/mL) and stored at −20°C before use.…”

“…This plant has been attributed with hepatoprotective, anticarcinogenic and antithrombotic properties (Morita et al, 2003). The methanol extract of M. malabarica has also been shown to have superoxide scavenging, prolyl endopeptidase inhibitory activity and antioxidant activity (Khanom et al, 2000;Patro et al, 2005). The chemopreventive action of mace on methyl cholanthrene induced carcinogenesis in the uterine cervix of mice has also been reported (Hussain and Rao, 1992).…”