Antioxidant Activity of Glyceollins Derived from Soybean Elicited with Aspergillus sojae

Abstract

The extract of soybean exposed to biotic elicitors such as food-grade fungus is known to have antioxidant activity. Glyceollins were major bioactive compounds present in soybean elicited by fungi and shown to have antifungal and anticancer activities. The purpose of present study was to evaluate the antioxidant activities of glyceollins by measuring ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, singlet oxygen quenching, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, hydroxyl radical scavenging activity, and lipid peroxidation inhibition. In addition, the antioxidant potential of glyceollins were measured by a fluorescent probe, 2',7'-dichlorofluorescin diacetate (DCFDA), and dihydroethidium (DHE) in mouse hepatoma hepa1c1c7 cells in which they were insulted with H2O2 to generate reactive oxygen species (ROS). Glyceollins showed a strong reducing power and inhibited lipid peroxidation, with significant scavenging activities of radicals including singlet oxygen, superoxide anion, ABTS, and DPPH. We also found that glyceollins significantly suppressed H2O2-induced ROS production in hepa1c1c7 cells. Therefore, glyceollins deserve further study as natural antioxidants and nutraceuticals.

Soyabean glyceollins: biological effects and relevance to human health

“…In addition, the antioxidant potential of glyceollins were measured by a fluorescent probe, 2,7-dichlorofluorescin diacetate, and dihydroethidium in mouse hepatoma hepa1c1c7 cells in which they were insulted with H 2 O 2 to generate ROS. The compound showed a strong reducing power and inhibited lipid peroxidation and significant scavenging activities of radicals including singlet oxygen superoxide anion, 2,2 0 -azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-diphenyl-1-picrylhydrazyl (13) . It was also found that glyceollins significantly suppressed H 2 O 2 -induced LDL oxidation production (Fig.…”

“…However, it is generally agreed that an excessive production of ROS is associated with ageing, cancer development, atherosclerosis and neurodegenerative disorders (39,40) . Glyceollins isolated from soyabean that sprouted in the presence of A. sojae showed strong antioxidant activity and ROS scavenging potential when assessed by an in vitro model (13) . The antioxidant activities of glyceollins were confirmed by measuring ferric reducing antioxidant power, 2,2-diphenyl-1-picrylhydrazyl radical scavenging, singlet oxygen quenching, 2,2 0 -azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging, hydroxyl radical scavenging activity and lipid peroxidation inhibition.…”

“…Our previous studies have provided evidence that glyceollins might have anti-carcinogenic and anti-atherosclerotic activities through their antioxidant potential (10)(11)(12) . Furthermore, glyceollins have been reported to suppress human breast and ovarian cancer formation through their anti-oestrogenic effect (13)(14)(15) . This review will discuss the potential health beneficial effects of glyceollins and their mechanisms of action.…”

Effects of glyceollin on mRNA expression in the female mouse brain.

“…When soybean tissues are challenged by a microbial or physical stimulus, a special class of low molecular weight plant defense compounds (phytoalexins) called glyceollins (Glys) are synthesized de novo and accumulate in the plant's tissues (Burden and Bailey 1975). Glys have received much attention due to their known antibacterial, antioxidant, antifungal, antitumor and insulinotropic actions both in vitro and in vivo (Kim et al 2011a;Kim et al 2010b;Ng et al 2011;Park et al 2010;Salvo et al 2006). The focus of this chapter is to review the known health-promoting effects of Glys and to discuss potential central nervous system effects that have not been previously described.…”

“…This work led to further research focusing specifically on the antioxidant activity of Glys. One study showed that a mixture of Gly I, II and III isomers exhibited strong antioxidant potential in mouse brain, liver and kidney extracts treated with hydrogen peroxide (H2O2), measured using radical ABTS and DPPH scavenging activity, reduction of ferric ion (Fe 3+ ) to ferrous ion (Fe 2+ ), inhibition of singlet oxygen formation and thiobarbituric acid reactive substances (TBARS) (Kim et al 2010b). Moreover, this…”