Antioxidant Activity of Flavones from Scutellaria baicalensis in Lecithin Liposomes

Abstract

Trihydroxyflavones of Scutellaria baicalensis, Antioxidant Activity, Liposome, Peroxidation, MDA The antioxidant effect of a trihydroxyflavone extract from Scutellaria baicalensis on oxida­ tion induced by ultraviolet light, was studied with phosphatidylcholine liposome membrane. Also, as standards, the antioxidative activity of baicalin, wogonin, baicalein and butylated hydroxytoluene (BHT) was investigated. Comparison of the protective effects of thecom­pounds studied against photoinduced lipid peroxidation in lecithin liposome membranes showed that: (1) the inhibitory effect of those compounds (at 1.2 mol% antioxidant content in liposomes) on TBA reactive materials from lipid peroxidation decreased in the order of baicalin > BHT ≅ Scutellaria baicalensis. These were found much greater than wogonin and baicalein; (2) the depressed effect of those compounds (at 1.1 mol% compounds content in liposomes) on the production of conjugated dienes (proportional to oxidation index) could be classified as follows: Scutellaria baicalensis ≅ baicalin > BHT, these three were found more active much greather than baicalein and wogonin. Results obtained by ESR measure­ment confirm that Scutellaria baicalensis extract and the BHT compound significantly de­ pressed the effect of liposome oxidation. It was found that the new trihydroxyflavones of Scutellaria baicalensis, ensured a very satisfactory concentration-dependent protection of the liposome membrane against UV-induced oxidation. These findings suggest that some of the beneficial effects of the extract of the Scutellaria baicalensis can be mediated in certain diseases (for example in skin diseases) by their ability to scavenge free radicals and by their protective effect on lipid peroxidation caused by sunlight irradiation.

Thermosensitive Hydrogel Mask Significantly Improves Skin Moisture and Skin Tone; Bilateral Clinical Trial

“…The flavonoids contained in S. baicalensis have also strong anti-oxidant properties [71]. An extract from this plant was shown to possess protective activity against oxidation induced by UV light [72][73][74] and it could be used as an additive in cosmetic products for UV protection [75]. Moreover, it was shown that topical baicalin application mitigates DNA photo-damage [76] and baicalein exerts anti-ageing effects through attenuating oxidative stress [77].…”

The effect of cholesterol on the adsorption of phenyltin compounds onto phosphatidylcholine and sphingomyelin liposome membranes

“…27 In brief, the lipid (egg phosphatidylcholine, sphingomyelin and cholesterol) with an appropiate amount of fluorescent probe were mixed in chloroform. After removing solvent, the dry lipid film was hydrated with 140 mM NaCl with phosphorane buffer at pH 7.4 (PBS).…”

Anthocyanin Extracts with Antioxidant and Radical Scavenging Effect

“…Egg lecithin in chloroform was dried under vac uum in a nitrogen atmosphere (Gabrielska et al, 1997). A 0.05 m M Tris:HCl [(hydroxymethyl) aminomethane] buffer of pH 7.4 was added and the sample was shaken for 15 min (control sam ple), and after adding the appropriate anthocyanin extracts for another fifteen min.…”

“…Egg lecithin was mixed with an appropriate amount of anthocyanin extract in chloroform, dried under vacuum in a nitrogen atmosphere (Gabrielska et al, 1997), and hydrated with a 0.05 m M TrisrHCl [(hydroxymethyl)aminomethane] buffer, pH 7.4. The sample was than shaken for 15 min.…”