Antioxidant activity of dihydrofurocoumarins from Corydalis heterocarpa

Kim, You Ah; Lee, Jung Im; Kong, Chang‐Suk; Choe, Joong Chul; Oh, Kwang-suk; Seo, Youngwan

doi:10.1007/s12257-014-0462-1

Cited by 7 publications

(4 citation statements)

References 31 publications

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“…n-BuOH fraction was subjected to silica gel and C-18 column chromatography, and five compounds ( 1 – 5 ) were isolated ( Figure 2 ). The spectroscopic data (ESI-MS, 1 H-NMR, and 13 C-NMR spectra) and comparisons with previous data confirmed that these structures were (−)-pinoresinol 4- O -β- d -glucopyranoside ( 1 ) [ 26 ], (−)-marmesinin ( 2 ) [ 27 ], columbianetin β- d -glucopyranoside ( 3 ) [ 28 ], isomucronulatol 7- O -β- d -glucoside ( 4 ) [ 29 ], and ecliptasaponin A ( 5 ) [ 30 ] ( Figure 2 , Supplementary Materials Figures S1–S15 ).…”

Section: Resultssupporting

confidence: 56%

Inhibition of Osteoarthritis-Related Molecules by Isomucronulatol 7-O-β-d-glucoside and Ecliptasaponin A in IL-1β-Stimulated Chondrosarcoma Cell Model

et al. 2018

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Osteoarthritis (OA) is the common form of arthritis and is characterized by disability and cartilage degradation. Although natural product extracts have been reported to have anti-osteoarthritic effects, the potential bioactivity of Ryupunghwan (RPH), a traditional Korean medicinal botanical formula that contains Astragalus membranaceus, Turnera diffusa, Achyranthes bidentata, Angelica gigas, Eclipta prostrata, Eucommia ulmoides, and Ilex paraguariensis, is not known well. Therefore, the inhibitory effects of single compounds isolated from RPH on the OA-related molecules were investigated using IL-1β-stimulated chondrosarcoma SW1353 (SW1353) cell model. Two bioactive compounds, isomucronulatol 7-O-β-d-glucoside (IMG) and ecliptasaponin A (ES) were isolated and purified from RPH using column chromatography, and then the structures were analyzed using ESI-MS, 1H-NMR, and 13C-NMR spectrum. The expression or amount of matrix metalloproteinase 13 (MMP13), COX1/2, TNF-α, IL-1β or p65 was determined by RT-PCR, Western blot, and enzyme-linked immunosorbent assay (ELISA). RPH pretreatment reduced the expression and amounts of MMP13, and the expression of collagen II, COX1/2, TNF-α, IL-1β or p65, which were increased in IL-1β-stimulated SW1353 cells. IMG reduced the expression of all OA-related molecules, but the observed inhibitory effect was less than that of RPH extract. The other single compound ES showed the reduced expression of all OA-related molecules, and the effect was stronger than that in IMG (approximately 100 fold). Combination pretreatment of both single components remarkably reduced the expression of MMP13, compared to each single component. These synergic effects may provide potential molecular modes of action for the anti-osteoarthritic effects of RPH observed in clinical and animal studies.

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Section: Resultssupporting

confidence: 56%

Inhibition of Osteoarthritis-Related Molecules by Isomucronulatol 7-O-β-d-glucoside and Ecliptasaponin A in IL-1β-Stimulated Chondrosarcoma Cell Model

et al. 2018

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“…Cell Culture and Determination of Intracellular Formation of Reactive Oxygen Species (ROS) Using DCFDA Labeling. Intracellular formation of ROS was assessed using 2′,7′‐dichlorofluorescin diacetate (DCFDA) as previously reported …”

Section: Methodssupporting

confidence: 88%

“…These findings supported that the cytotoxic effect of compound 6 against AGS cells induced apoptosis by p53 and Bax. 27 MTT Cytotoxicity Assay. Figure 1.…”

mentioning

confidence: 99%

An Unusual Homoisoflavanone from Portulaca oleracea

Lee

Kim

Kong

et al. 2019

Bulletin Korean Chem Soc

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“…[3][4][5] As a part of our search for new bioactive compounds from natural resources, we have recently reported the anti-proliferative activity of coumarins from C. heterocarpa on human cancer cells, as well as their antiinflammatory and antioxidant activities. [6][7][8][9] In this study, coumarins from C. heterocarpa were tested for their cytotoxic effect against human cancer cells. In addition, their possible action of mechanism was examined using human cancer cell line models.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a furochromone from the halophyte Corydalis heterocarpa for cytotoxic activity against human gastric cancer (AGS) cells

Kong

Kim

et al. 2016

Food Funct.

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A furochromone, heterocarpin (1), was isolated from the halophyte Corydalis heterocarpa, along with four known compounds (2-5), which were obtained for the first time from this genus. The chemical structures of these compounds were determined by extensive 2-D NMR experiments and by comparison with the data reported in the literature. Compound 1 showed significant cytotoxic activities against four human cancer cells, AGS (human gastric cancer), HT-29 (human colon cancer), HT-1080 (human fibrosarcoma) and MCF-7 (human breast carcinoma). According to the cytotoxicity results, the mechanism behind the cytotoxic presence of compound 1 on AGS cells was investigated through the mRNA and protein levels of apoptotic pathway factors such as p21, p53, Bax, Bcl-2, XIAP, and caspases-3 and -9. The results indicated that heterocarpin (1) showed the cytotoxic effect on cancer cells by inducing apoptosis via regulated Bax-Bcl-2 ratio, overproduced caspases and suppressed XIAP. The inhibition of NFκB and activation of JNK and ERK pathways were also observed in the presence of heterocarpin (1). Therefore, heterocarpin and its source C. heterocarpa were suggested to be utilized as a functional food with potential pro-apoptotic activity against cancer cells.

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Antioxidant activity of dihydrofurocoumarins from Corydalis heterocarpa

Cited by 7 publications

References 31 publications

Inhibition of Osteoarthritis-Related Molecules by Isomucronulatol 7-O-β-d-glucoside and Ecliptasaponin A in IL-1β-Stimulated Chondrosarcoma Cell Model

Inhibition of Osteoarthritis-Related Molecules by Isomucronulatol 7-O-β-d-glucoside and Ecliptasaponin A in IL-1β-Stimulated Chondrosarcoma Cell Model

An Unusual Homoisoflavanone from Portulaca oleracea

Evaluation of a furochromone from the halophyte Corydalis heterocarpa for cytotoxic activity against human gastric cancer (AGS) cells

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