Antioxidant Activity of Biotransformed Sex Hormones Facilitated by Bacillus stearothermophilus

Abstract

Bacillus stearothermophilus, a thermophilic bacterium isolated from Kuwaiti desert, when incubated with exogenous progesterone for 10 days at 65 degrees C produced two monohydroxylated, two dihydroxy isomers of progesterone and a B-Seco compound. These metabolites were purified by TLC and HPLC followed by their identification through (1)H, (13)C NMR and other spectroscopic data. Microbial hydroxylation of 17beta-estradiol resulted in the production of estrone. The effect of some inducers resulted in the production of two metabolites from 17beta-estradiol, one of which was identified as 3,6beta,17beta-trihydroxyestra-1,3,5,14(10)-tetrene and the other metabolite remains unidentified. The transformation products were identified through their spectral data and comparison with reference compounds. Antioxidant activities of progesterone transformed mixture and purified metabolites of 17beta-estradiol were studied by linoleic acid/beta-carotene assay. An enhanced antioxidant activity for progesterone transformation products was observed when compared to progesterone. A comparison of antioxidant activity of progesterone and 17beta-estradiol transformation products is reported.

Microalgal transformation of progesterone by the terrestrial-isolated cyanobacterium Microchaete tenera

“…6α-Hydroxyprogesterone is another natural occurring metabolite which is produced during the metabolism of progesterone, especially by fetal liver and placental enzymes during pregnancy (Lisboa and Gustafsson 1969). In the study of Afzal et al (2010), it was found that progesterone transformation products including 20α-, 6α-, and 6β-hydroxyprogesterone exhibit antioxidant activity more than progesterone alone. 6β-Hydroxylation has been reported by the isolated cytochrome P-450 enzyme and various Bacillus thermoglucosidasius (Sideso et al 1998;Wang et al 2000).…”

Potent Anti-Ovarian Cancer with Inhibitor Activities on Both Topoisomerase II and V600EBRAF of Synthesized Substituted Estrone Candidates

“…Synthetic alterations of estrone lead to discovering of compounds with diverse biological activities, for example with antitumor effect [1], as anti-breast cancer agent [2] and with antioxidant activity [3]. Estrone derivatives with antitumor activities must be devoid completely of the estrogenic activities [4,5,6].…”