Antioxidant Activity of Aqueous Extract of Leaves and Seeds of Datura metel (Solanaceae) in Frog’s Heart Failure Model

Hacheked, Mbida; Tsala, David Emery; Aboubakar, S.; Habtemariam, Solomon; Edmond, J. J.; Bakwo, E. F.; Minkande, Jacqueline Zé

doi:10.1155/2022/5318117

Cited by 4 publications

(3 citation statements)

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“…Importantly, compared with other species like D. stramonium, D. metel’s antioxidant property is quite high ( Iqbal et al, 2017 ). Antioxidant action is estimated by the aqueous extract of seed and leaf of D. metel at 2.5 and 1.5 mg/mL, respectively; they also found that it prolongs the cardiac arrest to 35 to 37 min, when the heart's normal survival time is 14 min ( Mbida et al, 2022 ). Four extract concentrations of four different solvents of D. metel extract 25–100 mg/mL were investigated in DPPH scavenging activity, total phenolic content, the reducing power assay, hydroxyl-radical scavenging, and β-carotene bleaching test.…”

Section: Resultsmentioning

confidence: 99%

Ethnobotanical uses and phytochemical, biological, and toxicological profiles of Datura metel L.: A review

Islam

Ara

Islam

et al. 2023

Current Research in Toxicology

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Section: Resultsmentioning

confidence: 99%

Ethnobotanical uses and phytochemical, biological, and toxicological profiles of Datura metel L.: A review

Islam

Ara

Islam

et al. 2023

Current Research in Toxicology

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“…This is supported by the administration of Leaves and Seeds of D. metel increased levels of superoxide dismutase, glutathione peroxidase, and catalase in Frog’s Heart Failure Model. 30 …”

Section: Discussionmentioning

confidence: 99%

Anti-Diabetic Effects of the 80% Methanolic Extract of Datura Stramonium Linn (Solanaceae) Leaves in Streptozotocin- Induced Diabetic Mice

Baylie,

Kebad,

Ayelgn

et al. 2023

JEP

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Background Managing diabetes mellitus with currently available drugs is costly, and the chances of side effects are high, leading to further studies for new and better medications from plant sources with the affordable and lower side effects. This study aimed to evaluate the anti-diabetic effects of Datura stramonium Linn (Solanaceae) Leaves Extract in Streptozotocin- Induced Diabetic Mice. Methods Male Swiss albino mice were induced into diabetes using 150mg/kg of STZ. Mice were allocated randomly into six groups, five mice per group. Group I was a normal control, Group II was Diabetic negative control, group III was Diabetic positive control, Group IV–VI were Diabetic Mice that treated with extract (100, 200 and 400 mg/kg) for 14 days. The FBG measurements were done on 0, 7th, and 14th days of treatment. After 14th day of treatment the mice were anesthetized with diethyl ether. Then, blood was drawn by cardiac puncture to assess TC, TG, LDL-C, and HDL-C. The antioxidant activity of the extract was determined using a DPPH assay. The data were entered into Epi-Data version 4.6, exported to SPSS version 26.0, and analyzed using a one-way ANOVA followed by a Tukey post hoc test. P < 0.05 was considered statistically significant. Results The extract of D. stramonium reduced the FBG level by 19.71%, 30.27%, 40.95%, and 45.67%, respectively, for D. stramonium 100, 200, 400, and GLC 5 mg/kg on the 14th day of treatment. Diabetic mice treated with D. stramonium for 14 days   showed a significant decrease in serum TC, LDL, and serum TG and a significant increase in body weight, and HDL level as compared to diabetic negative control. Antioxidant activities of the leaves extract were comparable to ascorbic acid with an IC50 of 172.79 μg/mL. Conclusion These findings revealed that the D. stramonium leaves extract possesses significant Anti-diabetic activities.

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“…As above mentioned, "hairy" root lines can be valuable not only due to high antioxidant activity, but due to exceptional reducing power as well. Most methods of reducing power evaluation are based on the ability of plant extracts to reduce radicals and metals [10][11][12][13][14][15][16][17]. DPPH (2,2-diphenyl-1picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) and NO (nitric oxide) tests are the most common in the first group, and FRAP (ferric reducing antioxidant power), FTC (ferric thiocyanate) and CUPRAC (cupric ions reducing assay) methods in the second.…”

Section: Introductionmentioning

confidence: 99%

Optical Characteristics of Silver Nanoparticles Obtained Using Artemisia tilesii Ledeb. "Hairy" Root Extracts With High Flavonoid Content

Bohdanovych

Матвєєва

2023

Innov Biosyst Bioeng

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Background. "Hairy" roots can be used for production of plant-derived secondary metabolites, such as flavonoids, which have antioxidant and reducing properties. It seems promising to use the process of silver nanoparticles formation as a method of determining the level of reducing power. This approach allows simultaneously to obtain nanoparticles with different biological activity and evaluate the reducing potential of different plants. Objective. The aim of the study was to determine the dependence of optical properties (ultraviolet/visible spectroscopy, UV-Vis spectra) of solutions of silver nanoparticles obtained using wormwood "hairy" root extracts on the content of flavonoids as compounds with reducing activity. Methods. Four Artemisia tilesii “hairy” root lines from the collection of the Institute of Cell Biology and Genetic Engineering NAS of Ukraine were grown for five weeks in liquid 1/2 MS medium with 20 g/l sucrose. "Hairy" roots were homogenized in 70% ethanol, the extracts were centrifuged, total flavonoid content was determined, and extracts were used for the silver nanoparticles preparation via reduction of Ag+ to Ag0 from AgNO3. UV-Vis spectra at 300–600 nm range were recorded right after colloid solution preparation, in five, and in nine days. Results. Total flavonoid content varied from 4.01 ± 0.39 to 15.37 ± 1.08 mg RE/g FW. The UV-Vis spectra curves of absorption increased with the course of time, mostly from day 0 to day 5. At this period, absorption at 370–500 nm wavelength increased almost twofold. The peak absorption of all samples was detected at 440 nm, and the maximum values at the wavelength of 440 nm correlated with the content of flavonoids. This correlation did not change over time. Conclusions. The optical properties of silver nanoparticles colloid solutions obtained using A. tilesii "hairy" root extracts correlated with the total flavonoid content of the samples. Even though the absorbance of the colloid solutions increased with time, those increases correlated with flavonoid content as well. The determination of the optical properties of AgNPs colloid solution can be used as a convenient way of quickly comparing the reducing ability of extracts both right after the formation of a colloidal solution and after some time of its storage.

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Antioxidant Activity of Aqueous Extract of Leaves and Seeds of Datura metel (Solanaceae) in Frog’s Heart Failure Model

Cited by 4 publications

References 25 publications

Ethnobotanical uses and phytochemical, biological, and toxicological profiles of Datura metel L.: A review

Ethnobotanical uses and phytochemical, biological, and toxicological profiles of Datura metel L.: A review

Anti-Diabetic Effects of the 80% Methanolic Extract of Datura Stramonium Linn (Solanaceae) Leaves in Streptozotocin- Induced Diabetic Mice

Optical Characteristics of Silver Nanoparticles Obtained Using Artemisia tilesii Ledeb. "Hairy" Root Extracts With High Flavonoid Content

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