Antioxidant activity in the leaves of Melilotus albus and Trifolium medium from man-made disturbed habitats in the Middle Urals under the influence of copper

Abstract

Physiological mechanisms of adaptation to copper induced stress in two widespread legume plants, white sweet clover (Melilotus albus Merik.) and zigzag clover (Trifolium medium L.), growing in habi tats differing in the man made pollution. An antioxidant plant defense system was activated in response to 10 mM CuSO 4 , which is a stress factor. Specific biochemical features related to adaptation to soil contamination with copper were observed in tested plant species. Superoxide dismutase was activated in response to stress in both species from various habitats. M. albus from the impact zone manifested the better capacity of proline accumulation as compared with plants from less polluted habitats. T. medium plants from the impact zone contained more active peroxidase. It was suggested that plants growing for a long time under stressful condi tions manifest the greater tolerance to copper ions than plants, which did not experience stress or were sub jected to the milder stress.

In Vitro Approbation of Microbial Preparations to Shield Fruit Crops from Fire Blight: Physio-Biochemical Parameters

“…A calibration curve was constructed to obtain a linear trendline equation (y = 0.0467 x − 0.0037; R 2 = 0.9926) for calculating protein concentration [ 57 ]. The activity of CAT, APX, and GR was calculated based on Beer’s–Lambert law [ 58 ], while the activity of SOD was determined according to Fazlieva et al [ 59 ]. A detailed description of the protocols and formulas used to determine the protein synthesis and antioxidant enzyme activity was shown in Nurzhanova et al [ 56 ].…”

Hydrogen Sulfide Donor Influence on State of Antioxidant System of Wheat Seedlings Under Osmotic Stress

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