Antioxidant Activity Evaluation of FlexirubinType Pigment from Chryseobacterium artocarpi CECT 8497 and Related Docking Study

Abstract

The current research is focused on studying the biological efficacy of flexirubin, a pigment extracted from Chryseobacterium artocarpi CECT 8497.Different methods such as DPPH, H2O2, NO•, O2•-, •OH, lipid peroxidation inhibition by FTC and TBA, ferric reducing and ferrous chelating activity were carried out to evaluate the antioxidant activity of flexirubin. Molecular docking was also carried out, seeking the molecular interactions of flexirubin and a standard antioxidant compound with SOD enzyme to figure out the possible flexirubin activity mechanism. The new findings revealed that the highest level of flexirubin exhibited similar antioxidant activity as that of the standard compound according to the H2O2, •OH, O2•-, FTC and TBA methods. On the other hand, flexirubin at the highest level has shown lower antioxidant activity than the positive control according to the DPPH and NO. and even much lower when measured by the FRAP method. Molecular docking showed that the interaction of flexirubin was in the binding cavity of the SOD enzyme and did not affect its metal-binding site. These results revealed that flexirubin has antioxidant properties and can be a useful therapeutic compound in preventing or treating free radical-related diseases.

Characterization of Bioactive Colored Materials Produced from Bacterial Cellulose and Bacterial Pigments

“…Moreover, the results showed that highest ABTS + scavenging activity was obtained for BC samples functionalized with flexirubin-type pigment, at 38.96 ± 0.49% ( Figure 5 B). The antioxidant activity of the flexirubin-type pigment, which can be attributed to the hydrogen atom transfer from the phenol, has been previously determined through different assays, besides the radical scavenging activities, namely lipid peroxide inhibition and ferrous chelating ability [ 20 ], which support the significance of flexirubin-type pigment as a powerful antioxidant, relevant for a wide range of applications.…”

“…Moreover, the yellow flexirubin-type pigment, which has been applied in the treatment of chronic skin disease, eczema, and gastric ulcers, also displays a wide range of pharmacological effects, which includes a high antioxidant potential [ 19 , 20 ]. In this study, the flexirubin-type pigment used was produced by Chryseobacterium shigense isolated from lactic acid beverage [ 21 ].…”

Interplay between the microalgae Micrasterias radians and its symbiont Dyadobacter sp. HH091

“…Another interesting point, is that on the plant-bacteria interaction model, flexirubin also performs as free radical scavenging antioxidant protecting from the attack of free radicals ( Combes and Finet, 1997 ; Schöner et al, 2015 ). The antioxidant potential via hydrogen donating ability of flexirubin has been shown through the assessment using different assays such as radical scavenging activities, lipid peroxide inhibition and ferrous chelating ability ( Mogadem et al, 2021 ). Several studies show that microalgae produce reactive oxygen species (ROS) to get an advantage in the competition for resources against other algae, be a way to prevent fouling bacteria, and act as a signalling mechanism between cells ( Marshall et al, 2005 ).…”

The Role of Flavobacterium enshiense R6S-5-6 in the Wetland Ecosystem Revealed by Whole-Genome Analysis

“…Flexirubin which are widely distributed in the genus Flavobacterium . They are pigments that form a yellow colony and are composed of a 2–5 dialkylresorcinol (DAR) with a non-isoprenoid aryl-polyene carboxylic acid ester [ 38 , 39 ]. Although their ecological roles are unknown, DAR compounds are known to have antibacterial properties in Pseudomonas sp.…”