Antioxidant Activity, Cytotoxicity, and DNA Information of Glossogyne tenuifolia

Abstract

This study investigates the antioxidant activity and cytotoxicity of Glossogyne tenuifolia extract on various cancer cell lines. The 5.8s DNA of G. tenuifolia was isolated, and the species of this plant was confirmed by NCBI's DNA database. G. tenuifolia was then extracted with ethanol and separated into several fractions using the partition procedure with water, n-butanol, and ethyl acetate (EA). Among these, the EA fraction most significantly affected the activity of DPPH(*) and superoxide anion scavenging. Additionally, only the EA fraction exhibited cytotoxicity on breast cancer cells (MCF-7 and MDA-MB-231) and liver cancer cells (Hep G2 and Hep 3B). Next, the EA fraction was further separated by column chromatography, and 15 fractions were obtained. Three effective components were isolated and identified separately from the active fractions: oleanolic acid (OA) from fraction 6, luteolin from fractions 8-10, and luteolin-7-glucoside from fraction 12. The test of these three compounds on scavenging activity of DPPH(*) and superoxide anion indicates that luteolin had the highest antioxidant activity, whereas the effect of OA was negligible. Additionally, a synergistic effect between luteolin and luteolin-7-glucoside was observed. Kick-out experiments showed that the activities were vanished or decreased. Especially on MDA-MB-231 and MCF-7 cells, the cytotoxicity completely disappeared when luteolin was eliminated from fractions 8-10. These findings demonstrate that luteolin plays a crucial role in the inhibition of the growth of hepatoma cancer cell lines. Fraction 3, which did not contain luteolin, luteolin-7-glucoside, and oleanolic acid, had cytotoxicity on MDA-MB-231, MCF-7, Hep G2, Hep 3B, and A549, which implies that this fraction contained some other effective ingredients and requires further study. The investigation is currently underway in our laboratory.

Antimicrobial and antioxidant activities with acute toxicity, cytotoxicity and mutagenicity of Cystoseira compressa (Esper) Gerloff & Nizamuddin from the coast of Urla (Izmir, Turkey)

“…The results indicated that algal extracts had no significant cytotoxic activity against Hep 3B cells in all treated concentrations (5-50 lg/mL). Previous studies indicated that the antioxidant activities may have synergistic effects on anticancer activity, proliferation inhibition, and apoptosis of human tumor or cancer cell lines (Seeram et al 2004;Shao et al 2004;Hsu et al 2005). In our study, the result of cytotoxicity has shown correlation between the results of antioxidant and cytotoxic activity since there was no cytotoxicity on Hep 3B cells with moderate antioxidant activity of the extracts.…”

Chemical Constituents, in Vitro Antioxidant Activity, Oral Acute Toxicity and Ld50 Determination of Moringa Oleifera Leaves

“…The synergistic effect (Co-activity) play a vital role during the estimation of antioxidant activity of plant extracts as a complex mixture containing a large number of combined compounds. These compounds may be reinforcing each other leading to raising their antioxidant potential and vice versa [20,29,30].…”

Hypoglycemic and Antioxidative Effects of <i>Glossogyne tenuifolia</i> on Streptozotocin-Nicotinamide-Induced Diabetic Rats

“…Some effective components from GT have been isolated and shown to exert combinatorial anti-inflammatory and antiviral effects in production of some mediators, i.e., TNF-alpha, IL-6 and IFN-gamma in activated human whole blood [10]. Its components, e.g., luteolin and luteolin-7-glucoside, play a crucial role in increasing scavenging capacity of free radicals and inhibiting the growth of hepatoma cancer cell lines [11].…”