Antioxidant activity, ascorbic acid, phenolic compounds and sugars of wild and commercial Tuberaria lignosa samples: Effects of drying and oral preparation methods

Abstract

The antioxidant activity and phytochemical composition (ascorbic acid, free sugars and phenolic compounds) of decoctions and infusions of wild and commercial samples of Tuberaria lignosa (Sweet) Samp. (Fam. Cistaceae) aerial parts were evaluated and compared. Among wild samples, the effects of the drying method (freeze or shadedrying) on those parameters were studied. Infusion of the freeze-dried wild sample gave the highest levels of sugars, while infusion of shade-dried wild sample and decoction of the freeze-dried sample presented higher ascorbic acid and phenolic compounds content (including ellagitanins and flavonoids) than the other samples. The last two samples also revealed higher antioxidant activity and, in some cases, even higher than trolox.Decoctions gave lower amounts of disaccharides than infusions, which seemed to be hydrolyzed increasing the content in monosaccharides in the first samples. Commercial samples showed the lowest content in phenolic compounds, mainly in ellagitanins and flavonoids, as also the lowest antioxidant activity. This work gives scientific evidences to the traditional medicinal uses of wild Tuberaria lignosa, highlighting the interest of its decoctions and infusions as bioactive compounds source and functional beverages.

Seeds of Opuntia spp. as a novel high potential by-product: Phytochemical characterization and antioxidant activity

“…The supernatant was concentrated at 60 ºC under reduced pressure and defatted three times with 10 mL of ethyl ether, successively. After concentration at 40 ºC, the solid residues were dissolved in water to a final volume of 5 mL and filtered through 0.2 µm nylon filters from Whatman (Pinela et al, 2012). The equipment of analysis consisted of an integrated system with a pump (Knauer, Smartline system 1000, Berlin, Germany), degasser system (Smartline manager 5000), autosampler (AS-2057 Jasco, Easton, MD) and a RI detector (Knauer Smartline 2300).…”

“…Fatty acids were determined after a transesterification procedure as described previously by the authors (Pinela et al, 2012), using a gas chromatographer (DANI 1000) equipped with a split/splitless injector and a flame ionization detector (FID at 260 ºC) and a Macherey-Nagel (Düren, Germany) column (50% cyanopropyl-methyl-50% phenylmethylpolysiloxane, 30 m × 0.32 mm i.d. × 0.25 µm d f ).…”

“…Tocopherols were determined following a procedure previously optimized and described by the authors (Pinela et al, 2012). Analysis was performed by HPLC (equipment described above), and a fluorescence detector (FP-2020; Jasco, Easton, MD, USA) programmed for excitation at 290 nm and emission at 330 nm.…”

Different Citrus rootstocks present high dissimilarities in their antioxidant activity and vitamins content according to the ripening stage

“…Successive dilutions were made from the stock solution and submitted to the in vitro assays already described by Martins et al (2014), to evaluate the antioxidant activity of the samples. The sample concentrations (mg/mL) providing 50% of antioxidant activity or 0.5 of absorbance (EC 50 ) were calculated from the graphs of antioxidant activity percentages (DPPH, β-carotene/linoleate and TBARS assays) or absorbance at 690 nm (ferricyanide/Prussian blue assay) against sample concentrations (Pinela et al 2012). Trolox was used as a positive control.…”

Phytopharmacologic preparations as predictors of plant bioactivity: A particular approach to Echinacea purpurea (L.) Moench antioxidant properties

“…The authors observed a higher antioxidant potential, phenolics and flavonoids contents in the wild samples; overall, freeze-dried preparation presented the most promissory antioxidant effects; however, while freeze-dried decoction preparation presented the highest abundance in flavonoids (19.48±0.50 mg/g), shade-dried infusion was more rich in phenolics (250.14±0.57 mg/g) [51].…”