Antioxidant Activity and Stability of Radish Bulbs (Raphanus sativus L.) Crude Extract

Eveline,; Pasau, Rani Lumiling

doi:10.1088/1755-1315/292/1/012036

Cited by 4 publications

(3 citation statements)

References 8 publications

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“…The increase of the activity as a function of the concentration of these compounds was also evidenced in the study of Park et al (2016) carried out with three cultivars of radish. Eveline and Pasau (2019) determined that the ethyl acetate would be the best type of solvent for the extraction of bioactive compounds in radishes, due to higher flavonoid concentrations and to a higher capability of neutralization of free radicals obtained. In previous studies with plants belonging to the Brassicaceae, the percentages of DPPH radical scavenged were found ranging from 14% to 23% in extracts of rocket (E. sativa) at a concentration of 0.1 mg/mL (ARBOS et al, 2010), 51.05% in broccoli extracts (B. oleracea var.…”

Section: Evaluation Of the Antioxidant Activitymentioning

confidence: 99%

Study and evaluation of antimicrobial activity and antioxidant capacity of dry extract and fractions of leaves of Raphanus sativus var. oleiferus Metzg.

et al. 2020

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The radish (Raphanus sativus L.) is a vegetable of the Brassicaceae family cultivated worldwide and has several medicinal properties. Its biological activities are related to various secondary metabolites present in the species, especially phenolics. Thus, the objectives of this study were the chemical analysis and evaluation of the antioxidant and antimicrobial activities of the dry extract and fractions of the fodder turnip leaves (R. sativus var. oleiferus Metzg.). Samples were analyzed by mass spectrometry and the antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical method and the reducing power method. Antimicrobial activity was determined by the agar diffusion and microdilution methods. The total phenols were concentrated in the butanol fraction (121.27 mg GAE/g) and the flavonoids were concentrated in the ethyl acetate fraction (98.02 mg EQ/g). The ethyl acetate fraction showed the best antioxidants results, with 83.45% of free radical scavenging and 11.34% of ferric ions reduction. The analysis of antimicrobial activity showed that the dry extract had the highest average zone of inhibition against Bacillus subtilis (18.67 mm). Smaller values of the minimum inhibitory concentration for Micrococcus luteus were, and the ethyl acetate fraction showed a lower minimum inhibitory concentration (0.1 mg/ml) for that microorganism. There was a strong correlation between the antioxidant activity and the content of phenols and flavonoids. The results showed the potential antioxidant and antimicrobial activities of this extract with the ethyl acetate fraction being most promising for further studies.

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Section: Evaluation Of the Antioxidant Activitymentioning

confidence: 99%

Study and evaluation of antimicrobial activity and antioxidant capacity of dry extract and fractions of leaves of Raphanus sativus var. oleiferus Metzg.

et al. 2020

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“…Antioxidant activities of R. sativus was previously demonstrated (Barillari et al, 2006;Beevi et al, 2012;Eveline & Pasau, 2019;Goyeneche et al, 2015;Kim et al, 2016;Noman et al, 2021;Shehzadi et al, 2020;Sonam, 2019). With an RSa50 value of 165.49 µg/mL, the methanol extract of R. sativus showed a better free-radical scavenging activity against DPPH than the 70% ethanol extract which presented an RSa50 value of 359.7 µg/mL in a previous study (Noman et al, 2021), but with a higher total phenol content of 95.8 GAE (compared to 6.17 GAE found in this study).…”

Section: Discussionmentioning

confidence: 92%

Radical Scavenging Activities, Total Reducing Power, Total Phenolic and Flavonoids Contents of Four Common Vegetables

Djeussi

Kenfack

Anangmo

et al. 2022

EJBIO

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Aim: This study aimed at evaluating the antioxidant activities of extracts from four vegetables namely: Beta vulgaris, Raphanus sativus, and two varieties (red and green) of Brassica oleracea. Method: The antioxidant properties of these extracts were assessed using five different methods including 2,2-diphényl 1-picrylhydrazyl (DPPH), Nitrogen Oxyde (NO), total reducing power, total phenolic and flavonoids content. Results: All the four vegetable extracts showed free radical-scavenging activity against DPPH· with RSa50 (Radical scavenging activity 50) ranging between 129.77 and 323.64 µg/ml, and inhibitory activity against NO radical (RSa50 ranging from 1454,52 to 4479,97 µg/ml). The four vegetable extracts also showed total reducing powers ranging between 2.41 and 9.37 AAE (mg ascorbic acid equivalents per gram of dried extract). These antioxidant activities can be justified by the presence of different antioxidant compounds like total phenol contents which were present in all studied vegetable extracts with quantities varying between 4.37 and 11.83 GAE (mg of garlic acid equivalents per gram of dried extract) of dry extract, or flavonoids which were also present in all the plants with total contents ranging between 0.1 and 0.25 RE (rutin equivalents per gram of dried extract). Conclusion: The different antioxidant activities demonstrated in this study provide scientific evidence that some vegetables commonly consumed in Cameroon including B. oleracea, R. sativus and B. vulgaris can serve as a dietary supplement or in preventive medicine in the management of oxidative stress and associated pathologies.

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“…Radish was purchased from a local supermarket in Pagoh, Johor and the radish powder was prepared following Eveline and Pasau (2019) with slight modification. All chemicals were of analytical grade, and purchased from QRëC (New Zealand), except for calcium chloride trihydrate (HmbG, Germany).…”

Section: Methodsmentioning

confidence: 99%

Formulation of halalan toyyiban radish effervescent tablet

Azahar,

Muhammad,

Abdul Rahim

2023

IFRJ

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Radish is a vegetable high in nutritional and phytochemical contents that are beneficial to the human body, especially for managing kidney stone disease. However, it is less consumed as compared to other vegetables due to its pungent flavour and odour. Therefore, the objective of the present work was to formulate a radish effervescent tablet to enhance the palatability of the vegetable. The radish effervescent tablet was formulated by using Simplex Lattice Mixture Design where the percentages of sucralose and strawberry flavour were chosen as the factors. Five formulations were generated, and sensory acceptance test was conducted on them. Next, the halalan toyyiban principle compliance was evaluated based on the Halal Control Points (HCPs), toxicity assay (brine shrimp lethality assay), anti-urolithiatic properties (turbidimetric assay), and nutritional composition (energy, total protein, total fat, total carbohydrate, and total sugar). Formulation 5 (F5) with 20% citric acid, 12% sodium bicarbonate, 58% radish powder, 2.5% sucralose, and 7.5% strawberry flavour was selected as the most acceptable formulation (p < 0.05). For the halalan toyyiban principle compliance, F5 was evaluated, and it complied with the halalan toyyiban principles. It is halal, non-toxic, and safe for consumption as the LC50 was greater than 1,000 μg/mL (2,223.31 μg/mL) for toxicity test, and exhibited significant potential as an anti-urolithiatic agent (88.13% inhibition). In the future, prototype development of radish effervescent tablet with potential anti-urolithiatic and fast-dissolving properties can be carried out.

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Antioxidant Activity and Stability of Radish Bulbs (Raphanus sativus L.) Crude Extract

Cited by 4 publications

References 8 publications

Study and evaluation of antimicrobial activity and antioxidant capacity of dry extract and fractions of leaves of Raphanus sativus var. oleiferus Metzg.

Study and evaluation of antimicrobial activity and antioxidant capacity of dry extract and fractions of leaves of Raphanus sativus var. oleiferus Metzg.

Radical Scavenging Activities, Total Reducing Power, Total Phenolic and Flavonoids Contents of Four Common Vegetables

Formulation of halalan toyyiban radish effervescent tablet

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