Antioxidant activity and protection against oxidative-induced damage of Acacia shaffneri and Acacia farnesiana pods extracts: in vitro and in vivo assays

Abstract

BackgroundObesity is a worldwide public health issue, reaching epidemic condition in developing countries associated to chronic diseases. Oxidative damage is another side effect of obesity. Antioxidant activity from plant components regulates at some extent this imbalance. Main goal of the present study was to determine the antioxidant activity and protection against oxidative-induced damage of Acacia shaffneri (AS) and Acacia farnesiana (AF) pods extracts.MethodsTo evaluated antioxidant activity and radical scavenging capacity of AS and AF extracts, two experiments were performed: 1) pods extracts were challenged against H2O2 using kidney cells in an in vitro assay; and 2) (Meriones unguiculatus) was employed in an in vivo assay to observe the effect of pods extracts on scavenging properties in plasma.ResultsBoth pods extracts presented an important protective effect on radical scavenging capacity against ABTS• + and DPPH+, and also in TBARS formation in vitro. Vegetal pods extracts did not induce any pro-oxidative effect when added to kidney cells in DMEM. Cells damage in DMEM with addition of H2O2 was significantly higher than those when vegetal pods extracts were added at 50 (P < 0.05) or 200 ppm (P < 0.001). Plasma scavenging properties presented an important dose-dependent positive effect in those groups where pods extracts were administered.ConclusionsThe antioxidant protection of the acacia pods extracts reported in this study suggests the possible transference of antioxidant components and protective effects to animal products (milk, meat, and by-products) from Acacia pods when this vegetation is included in the diet. In order to evaluate, the possible transference of theirs antioxidant components to animal products, the incorporation of these non-conventional resources to ruminant feeding is a good opportunity of study. Profiling of Acacia farnesiana pods extract is necessary to identify the responsible bioactive compounds of protective properties.

Acacia hydaspica R. Parker ameliorates cisplatin induced oxidative stress, DNA damage and morphological alterations in rat pulmonary tissue

“…7- O -galloyl catechin, +catechin and methyl gallate are the main bioactive metabolites with anticancer potential against breast and prostate cancer [ 27 – 29 ]. Various species of genus Acacia were reported for their antioxidant and protective potentials against lung toxicity in animal models [ 31 ]. A. hydaspica AHE fraction showed significant hepato-protective potential against cisplatin persuaded hepatic damage in rats [ 32 ].…”

Doxorubicin-induced alterations in kidney functioning, oxidative stress, DNA damage, and renal tissue morphology; Improvement by Acacia hydaspica tannin-rich ethyl acetate fraction

“…Bioassay-guided isolation identified 7-O-galloyl catechin, catechin, catechin gallate, and methyl gallate as major antioxidant and anticancer phytoconstituents from A. hydaspica ethylacetate fraction (AHE) ( Afsar et al, 2016a , Afsar et al, 2018 , Afsar et al, 2016 ). Genus Acacia exposed antioxidant and nephroprotective proficiencies in animal prototypes ( Puga et al, 2015 ). The aqueous extract of A. Senegal showed significant nephroprotective potency against gentamicin (100 mg/kg) induced renal damage by lowering serum creatinine and urea levels in rats ( Mohammed, 2015 ).…”

Effects of Power Ultrasound on Stability of Cyanidin-3-glucoside Obtained from Blueberry

“…This phenomenon was caused by cavitation during power ultrasound, causing molecules to produce ·OH, which in turn attacked Cy-3-glu, resulting in the lost ability of the latter to scavenge free radicals. Thus, the ability of Cy-3-glu to remove ·OH decreased [23,24]; the greater the intensity of power ultrasound, the more severe the damage would be.…”

“…Consistent with the result of spectral analysis, the residual anthocyanins after power ultrasound remained to be in the form of salt cationic (AH + , red). The degradation products were not hydroxybenzoic acid, hydroxyl-benzaldehyde, and chalcone, so the antioxidant activity did not [22,23]. Turfan [19] found that the main mechanism of anthocyanin degradation by H 2 O 2 includes three reactions: ·OH generation and induction of the benzene of anthocyanins to generate CO 2 and H 2 O [24].…”