Antioxidant activity and phenolic profiles of the wild currantRibes magellanicumfrom Chilean and Argentinean Patagonia

Abstract

The Patagonian currant Ribes magellanicum is highly valued due to its pleasant flavor and sweet taste. The aim of this study was to characterize its constituents and to assess their antioxidant and cytoprotective properties. For the fruit phenolic‐enriched extract (PEE), total phenolics (TP), total flavonoids (TF), and antioxidant activity (DPPH, Ferric reducing antioxidant power (FRAP), and Trolox equivalent antioxidant activity (TEAC)) were determined. Argentinean samples presented better activity in the DPPH and FRAP assays. Best cytoprotection against oxidative stress induced by H2O2 in AGS cells was found in one Argentinean sample at 500 μg mL−1 (65.7%). HPLC MS/MS analysis allowed the tentative identification of 59 constituents, including eight anthocyanins, 11 conjugates of caffeic‐, ferulic‐, and coumaric acid, and 38 flavonoids, most of them quercetin and kaempferol derivatives. Argentinean samples showed a more complex pattern of anthocyanins, hydroxycinnamic acids (HCA), and flavonoids. Cyanidin rhamnoside hexoside and cyanidin hexoside were the main anthocyanins, accounting for 35 and 55% for the Argentinean and 60 and 27% for the ripe Chilean fruits. HCA content was about three times higher in Argentinean samples. The phenolic profiles of Chilean and Argentinean Ribes magellanicum show remarkable differences in chemical composition with higher HCA and flavonoid content in Argentinean samples.

Cytoprotective Mechanisms Mediated by Polyphenols from Chilean Native Berries against Free Radical‐Induced Damage on AGS Cells

“…The phenolic composition of the PEEs from R. geoides , Ribes spp., and F. chiloensis has been previously reported. The PEEs from the selected berries present antioxidant activity, but the chemical composition of the fruit phenolics is very different, as can be expected from different fruit (berries) species [ 9 , 16 , 24 – 26 ]. The PEE of Fragaria chiloensis presents hydrolizable and condensed tannins as well as flavonoids, while Ribes spp.…”

“…The study was performed with the polyphenolic-enriched extracts (PEEs) of the fruits. The PEEs were obtained according to the methodology previously reported [ 9 , 24 – 26 ]. Briefly, the fruits were homogenized and extracted with a MeOH : formic acid 99 : 1 ( v / v ) mixture and the solution was taken to dryness under reduced pressure.…”

“…The resin was washed with distilled water, and the polyphenols were desorbed with MeOH to afford after concentration under reduced pressure and lyophilization of the phenolic-enriched extracts (PEEs). The chemical characterization of the PEEs has been reported as follows: Ribes magellanicum [ 16 , 24 ], Rubus geoides [ 9 ], and Fragaria chiloensis ssp. chiloensis f .…”

Polyphenol Composition and (Bio)Activity of Berberis Species and Wild Strawberry from the Argentinean Patagonia

“…The (bio)activity and chemical profiles of Patagonian wild berries have been carried out mainly for Chilean collections [18,19,20,21,22]. Differences in the composition and bioactivity of Patagonian berries occurring in both sides of the Andes Mountains have been described [18,23], and additional differences should be expected.…”

“…The (bio)activity and chemical profiles of Patagonian wild berries have been carried out mainly for Chilean collections [18,19,20,21,22]. Differences in the composition and bioactivity of Patagonian berries occurring in both sides of the Andes Mountains have been described [18,23], and additional differences should be expected. The aim of the present work was to describe the polyphenol content and composition, antioxidant, and inhibitory activity towards metabolic syndrome-associated enzymes of different populations from Argentinean Patagonia berries (“calafates” and wild strawberry), and to compare the results with those reported for the Chilean samples.…”