Antioxidant Activity and Hepatoprotective Effects of Extracts from Different Parts of Papaver rhoeas L.

Abstract

This study examined the antioxidant activities of the two Papaver rhoeas L. (PR; PRP (pink flower PR), PRR (red flower PR)) on hydrogen peroxide (H2O2)-induced oxidative stress in HepG2 cells. Different parts (whole, leaf, and fruit) of PRP and PRR were extracted with 50% ethanol. Their antioxidant activities were analyzed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzoline-6-sulfonic acid) (ABTS) radical scavenging assay. The extracts were evaluated for cellular anti-oxidation and protection from the cell viability, intracellular reactive oxygen species (ROS) scavenging activity and antioxidant enzyme activities. The PR leaf and fruit extracts contained high amounts of bio-active compounds, such as total phenol and total flavonoid. The DPPH and ABTS results revealed antioxidant activity in the following order: leaf, fruit, and whole plant. The pretreatment with all the PR extracts increased the viability, antioxidant enzyme activities and intracellular ROS scavenging activity in a dose-dependent manner in H2O2-treated HepG2 cells. In particular, intracellular ROS scavenging activity was highest using the leaf extracts. These results suggest that 50% ethanol extracts of PR could be used as functional food sources.

Papaver species: usage areas, essential oil, nutrient and elements contents, biological activities

“…It has been reported that methanol extract of P. rhoeas collected from Slovakia has potent effects against TK6 human lymphoblastoid cell line in the highest used concentration, in lower concentration it has stimulating effect [46]. In a study reported from Korea, ethanol extract of whole, leaf, and fruit parts of P. rhoeas was reported to be effective against HepG2 cell lines [55]. It has been reported that alkaloid extracts of Papaver somniferum collected from Germany are effective against HeLa, Caco-2, MCF-7, CCRF-CEM and CEM/ADR 5000 [63].…”

“…It has been reported that the ethanol extracts and ethyl acetate fractions of Papaver rhoeas and Papaver nudicaule collected from Kore exhibit high antioxidant potentials as determined by DPPH and nitric oxide scavenging tests[42]. The ethanol-water extracts of has antioxidant activity in DPPH and ABTS tests[55]. EC50 values of DPPH test result of methanol extract of P. somniferum collected from Germany, whole plant, roots, capsule, seed, flowers, stems, leaves and alkaloid extracts were in the range of 7.4-157.6 μg/mL, EC50 values of ABTS test ranged 8.1-306.3 μg/mL and the result of the FRAP test was reported to be 750.1-1348.7 mM FeSO4/g [63].…”