Antioxidant Activity and Chemical Composition of Extracts from Fruiting Bodies of Xylotrophic Fungi Growing on Birch

Ermoshin, Alexander; Kiseleva, Irina; Nikkonen, Irina; Nsengiyumva, Dominique S.; Duan, Shen; Ma, Cui; Курченко, В. П.

doi:10.17516/1997-1389-0354

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…Phenolic compounds were extracted by 70% ethanol, and their concentration was determined using the Folin-Ciocalteu reagent and performed in µg g −1 FW in terms of gallic acid [22]. The content of Klason lignin (KL) and acid-soluble lignin (ASL) were determined in the dry ground roots or stems by the sulfuric acid method [23] and expressed in percentages (%).…”

Section: Biochemical Characteristicsmentioning

confidence: 99%

Copper Stress Enhances the Lignification of Axial Organs in Zinnia elegans

et al. 2022

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Zinnia elegans Jacq. is an ornamental plant, widely used in landscaping. Heavy-metal pollution in urban and rural areas is still increasing, which determines the actuality of studying plants’ reactions to pollutants. Zinnia was not sufficiently studied in this regard, so the aim of our research was to identify morphophysiological changes in this species under excess copper concentration in the soil. For this, we treated a growth substrate with 200 µM CuSO4 solution for 20 days. At the end of the treatment, several morphological, biochemical, and molecular genetic traits were evaluated: the root and the shoot size; the concentration of H2O2 and malondialdehyde (MDA), as indicators of stress; the amount of the phenolic compounds and lignin; and the level of the expression of genes, which encoded their biosynthesis. The Cu amount in the substrate and zinnia organs was quantified using atomic-absorption spectroscopy; hydrogen peroxide, MDA, and phenolic compounds were determined spectrophotometrically, while the amount of lignin was determined according to Klason. Real-time PCR was used for estimation of the gene-transcription level. Lignin in tissues was visualized by fluorescent microscopy. In experimental plants, Cu accumulation was higher in the root than in the stem. This caused an increase in stress markers and a decrease in the root and stem lengths. For the first time for zinnia, it was shown that for several genes—4-coumarate-CoA ligase (4CL), cinnamoyl alcohol dehydrogenase (CAD), and class III peroxidase (PRX)—the level of expression increased under copper treatment. The rise of the transcripts’ amount of these genes was accompanied by a thickening and lignification of the cell walls in the metaxylem vessels. Thus, the adaptation of zinnia to the excess Cu in the growth medium was associated with the metabolic changes in the phenylpropanoid pathway. As a result, the lignification increased in the root, which led to the accumulation of Cu in this organ and limited its translocation through the xylem to the stem, which provided plant growth.

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Section: Biochemical Characteristicsmentioning

confidence: 99%

Copper Stress Enhances the Lignification of Axial Organs in Zinnia elegans

et al. 2022

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“…Bark chemistry was analyzed using thin layer chromatography (Wagner et al, 1984;Ermoshin et al, 2021) in a solvent system toluene:ethyl acetate:formic acid, 30:18:2. One hundred milligrams of bark of six tree species in 3 repeats each were extracted in 1 mL acetone and 14 mkL of every extract was applied on a chromatographic plate.…”

Section: Analytical Proceduresmentioning

confidence: 99%

The Role of Secondary Metabolites and Bark Chemistry in Shaping Diversity and Abundance of Epiphytic Lichens

Paukov

Teptina

Ermoshin

et al. 2022

Front. For. Glob. Change

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Diversity of secondary lichen metabolites was studied in epiphytic lichens on six phorophytes—spruce, pine, birch, alder, aspen and poplar in the Middle Urals of Russia. Atranorin, usnic, fumarprotocetraric acid, zeorin, and gyrophoric acid were found in 31, 24, 23, 18, and 14 species, respectively, of 237 taxa collected. Seventy-seven species (i.e., 32% of total species documented) contained no secondary metabolites. Spectra of secondary metabolites of fruticose and foliose lichens varied on different phorophytes, while in crustose species the strong dependence on the tree species was not detected. This is different to the pH dependence of saxicolous lichens where crustose lichens were more susceptible to the rock chemistry. The results of Canonical Correspondence Analysis reveal the affinity of species containing depsides, depsidones or usnic acid to acidic substrata and those lacking secondary metabolites or containing terpenes and antraquinones to the pH-neutral bark. We suppose that phenolic compounds and flavonoids, as chemical constituents of bark, may interact with lichen symbioses and elements in phellem, and similarly to the lichen acids shape the affinity of species to the substrata.

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