Antioxidant activity and characterization of flavonoids and phenolic acids of Ammoides atlantica by RP–UHPLC–ESI–QTOF–MSⁿ

Abstract

In this study, phytochemical profiling of hydro-alcoholic extract of Ammoides atlantica aerial parts has been carried out using RP-UHPLC-ESI-QTOF-MS in negative ionization mode Chemical characterization was established according to the MS and MS/MS spectra. A total of 66 chemical compounds were detected. Among these, 45 compounds were identified: hydroxycinnamic acids and derivatives (26), hydroxybenzoic acids (4), flavones (11), flavonols (3), and a lignan. Total phenolics (371.57 mg/g) and total flavonoids (41.02 mg/g) contents were also determined. Moreover, the antioxidant activity of A. atlantica extract was also studied by six methods: DPPH (IC 50 : 23.31 µg/mL), ABTS + (IC 50 : 11.31 µg/mL), O 2 -DMSO alkaline (IC 50 : 3.19 µg/mL), ferrous ions chelating assays (IC 50 : 102.35 µg/mL), reducing power (A 0.50 : 92.70 µg/mL) and CUPRAC (A 0.50 : 13.56 µg/mL) assays. These results suggest that the antioxidant activity of the hydroalcoholic extract was comparable to common antioxidant additives in most of the tests, representing a good alternative.

Metal Chelating and Cupric Ion Reducing Antioxidant Capacities of Ammoides atlantica Aqueous Extract

“…The phenolic compounds acting as hydrogen donors, free radical acceptors, chain oxidation reaction interrupters or metal chelators 22 . This finding of the antioxidant capacity of Ammoides atlantica is in agreement with other studies 23,24,25 .…”

Use of High-Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry for Structural Characterization of Bioactive Compounds in the Olive Root Bark and Wood of Chemlali Cultivar

“…It is relevant to underline the strong correlation between the antioxidant activity and polyphenol concentration and chemical structure. 6 , 42 Notably, flavonoids, secoiridoids, and hydrophilic phenols, such as phenolic acids and phenolic alcohols, are responsible for the high antioxidant activity in olive oil and olive byproducts. 7 , 16 , 42 …”

Chemical Composition and Antioxidant Activity of Ammi visnaga L. Essential Oil

“…Concerning the in vitro reducing power, essential oil showed good activity at an EC 50 = 730.53 ± 0.017 µg/mL, although this is relatively lower when compared to other species within the Apiaceae family, such as the hydroalcoholic extract of the aerial parts of Ammoides atlantica with an IC 50 : 92.70 ± 1.00 µg/mL, the Tunisian Pimpinella anisum during maturation (EC 50 = 0.523 mg/mL), or the ethanolic seed extract of Trachyspermum ammi , with a reducing power ability at an optic density of 0.91 at 25 μg/mL [ 51 , 52 , 53 ].…”