Antioxidant activity analysis of collagen peptide-magnesium chelate

Structural characterisation of deer sinew peptides as calcium carriers, their promotion of MC3T3-E1 cell proliferation and their effect on bone deposition in mice

“…Fluorescence spectroscopy allows exploration of the interactions between organic ligands and metal ions by detecting changes in the wavelength and fluorescence intensity in the spectrum, and tracking changes in the folding or unfolding of peptides. 44 Trp, Phe, and Tyr are intrinsically fluorescent, and these three amino acids have different fluorescence excitation and emission spectra due to the differences in their side chain chromophores. 45 It can be seen from Fig.…”

Differential impact of the triple helix dissociation degree of collagen on the binding efficiency with cancer cells and normal cells

“…Briefly, 10 μL of CCK-8 solution was introduced into each well, and the cells were incubated at 37 °C for 4 h. The absorbance at 450 nm was measured to determine the cell viability. 33,34…”

“…Briefly, 10 mL of CCK-8 solution was introduced into each well, and the cells were incubated at 37 1C for 4 h. The absorbance at 450 nm was measured to determine the cell viability. 33,34 2.4.6 Scratch assay. Cells were seeded into collagen-coated wells as previously described.…”

Identification of novel antioxidant collagen peptides for preventing and treating H2O2‐induced oxidative stress in HepG2 cells through in vitro and in silico approaches

“…The plate was further incubated at 37 °C for 2 h and the absorbance at 450 nm was measured. Then, the H 2 O 2 ‐induced oxidative stress model was established according to a previously reported method in the literature, 34 to verify the protective effects of synthetic peptides (12.5–200 μmol L −1 ) on HepG2 cells. In the prevention group, cells were pre‐treated with samples for 24 h and exposed to H 2 O 2 (600 μmol L −1 ) for 6 h. In the repair group, cells were pre‐incubated with H 2 O 2 and then treated with samples.…”