Antioxidant Activity, Alpha-Glucosidase Inhibitors Ethanol Extract of Vernonia amygdalina Del. and Nutraceutical Potential

Abstract

Vernonia amygdalina Del is a shrub that is very well known in African continent and usually grows in tropical climates, including in Indonesia. In this research, we tested the The ethanol extract's anti-oxidant properties of Vernonia amygdalina Del leaves and the inhibitory activity of alpha-glucosidase. Vernonia amygdalina Del leaves ethanol extract was obtained using Ultrasound Assisted Extraction (UAE) method by variation of time and amplitude. Antioxidant activity of Vernonia amygdalina Del leaves ethanol extract was tested using DPPH and CUPRAC methods. Based on the research, IC50 of Vernonia amygdalina Del leaves ethanol extract was obtained by DPPH method respectively that is A (111.72±0.10)mg/L, B (154.08±0.02)mg/L, C (191.22±0.04) mg/L and D (190.99±0.08) mg/L. While IC50 of the Vernonia amygdalina Del leaves ethanol extract by CUPRAC method are A (26.71±4.85), B (78.57±5.04), C (43.50±4.62) and D (76.60±1.79). In this study, an alpha-glucosidase inhibition test was carried out where the greatest inhibitory activity was obtained by the Vernonia amygdalina Del leaves ethanol extract with variation A (30 minute extraction time with a 0.60 m amplitude) and IC50 is (0.69±0.13)mg/L. Based on this study, it can be concluded that Vernonia amygdalina Del has potential as an antidiabetic and nutraceutical drug.