Antioxidant Activities of Solanum nigrum L. Leaf Extracts Determined in In Vitro Cellular Models

Abstract

Several medicinal foods abound in traditional medicine with antioxidant potentials that could be of importance for the management of several diseases but with little or no scientific justification to substantiate their use. Thus, the objective of this study was the assessment of the antioxidant effect of two leave extracts of Solanum nigrum L. (SN), which is a medicinal plant member of the Solanaceae family, mainly used for soup preparation in different parts of the world. Then methanolic/water (80:20) (SN1) and water (SN2) leaves extracts were prepared. The total polyphenolic content and the concentration of phenolic acids and flavones compounds were determined. In order to verify whether examined extracts were able to restore the oxidative status, modified by glutamate in primary cultures of astrocytes, the study evaluated the glutathione levels, the intracellular oxidative stress, and the cytotoxicity of SN1 and SN2 extracts. Both extracts were able to quench the radical in an in vitro free cellular system and restore the oxidative status in in vitro primary cultures of rat astroglial cells exposed to glutamate. These extracts prevented the increase in glutamate uptake and inhibited glutamate excitotoxicity, which leads to cell damage and shows a notable antioxidant property.

Antiviral effect of Chinese herbal prescription JieZe-1 on adhesion and penetration of VK2/E6E7 with herpes simplex viruses type 2

“…S. nigrum L., bitter and sweet in flavor, slightly cold in property, acting on the lung and urinary bladder channels with the effects of clearing away heat, removing toxicity, detumescence, and relieving itching, mainly treating carbuncle, eczema, and dermatitis. S. nigrum L. is wildly used in TCM for the treatment of a wide range of diseases including some eye infections, seizure, epilepsy, inflammation, pain, diarrhea, ulcer, and jaundice (Campisi et al, 2019). T. mongolicum Hand.-Mazz, bitter and sweet in flavor, cold in property, acting on the liver and stomach channels with the effects of clearing away heat, removing toxicity, reducing swelling, and dissipating mass.…”

Untitled

“…Antioxidant properties of medicinal plants play an important role in Ayurvedic treatments due to their multifaceted action. The significant positive correlation between phenols, flavonoids with different antioxidant assays such as DPPH, RP, and CUPRAC indicated that these bioactive compounds play a role in the antioxidant property of the plant 32,33 . Earlier reports suggested that phenolic compounds concentrated in the fruit skin contribute to their antioxidant property.…”

Tanqiya wa Tadeel (Evacuation and Rejuvenation): The Unani Concept as Evolutionary Basis for Conventional Stroke Management

“…Neurodegeneration is supposed to be potentiated with uncontrolled production of free radicals which can be controlled up to some extent with external antioxidants. Inabussalab (Solanum nigrum Linn) is reported to have significant antioxidant and anti-inflammatory activities [45][46][47] . Anti-inflammatory action of Beikh-e-Kibr (Capparis spinosa Linn) has been proved in various reports.…”