Antioxidant Activities of<i>Crataegus monogyna</i>Extracts

Bahorun, Theeshan; Trotin, F; Pommery, Jean; Vasseur, J.; Pinkas, M

doi:10.1055/s-2006-959493

Cited by 106 publications

(88 citation statements)

References 9 publications

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“…Unlike the three fruit extracts (HE, HP, and HJ), the main polyphenolic components of HF were two C-glycosylflavones, of which vitexin-2 00 -rhamnoside was the more abundant (20.5%, w/w). This flavonoid profile was similar to those seen for European hawthorn leaves, 31) but this extract lacked procyanidins and other phenolics.…”

Section: Contents Of Organic Acid and Polyphenolssupporting

confidence: 75%

“…32) Many of the chemoprotective effects of phenolics, such as procyanidins, are related to the scavenging actions of oxygen radicals, and include resistance to lipid peroxidation, 31,33) antioxidation of low density lipoproteins, 4,5,34) and protection against damage from UV or -ray irradiation. 35,36) Our results regarding the scavenging capacities of hawthorn extracts and related compounds for O 2 À and OH radicals are shown in Table 2.…”

Section: Antioxidant Activities Of Hawthorn Extracts and Related Compmentioning

confidence: 99%

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Polyphenolic Content and Physiological Activities of Chinese Hawthorn Extracts

Cui

Nakamura

Tian

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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Hawthorn polyphenol (HP) was prepared by ethyl acetate treatment of the ethanol extract (HE) of Chinese hawthorn fruit. The concentrations of 15 polyphenols in the HP, HE, extraction residue (HJ), and a hawthorn leaf extract (HF) were determined by HPLC. For HP, the total content of the 15 polyphenols was 21.4%, comprised of 19.7% of procyanidins, 1.21% of chlorogenic acid, and 0.48% of flavonoids, compared to 2.55% for the HE. The yields of procyanidin monomer, dimer, trimer, tetramer, and pentamer were 50.5%, 30.3%, 23.0%, 14.6%, and 12.5% respectively, and the mean degree of polymerization was reduced to 1.39 (HP) from 1.65 (HE). Seven different physiological actions of the four extracts were investigated. The HP showed strong O 2 À and OH scavenging capacities (IC 50 values of 6.3 g/ml and 1.1 g/ml respectively), as well as selective prolyl endopeptidase inhibition (IC 50 of 60 g/ml). The active constituents appeared to be procyanidins.Key words: Crataegus pinnatifida; HPLC; procyanidin; antioxidant; free radicalsIn Europe, standardized extracts of the leaves and flowers of Crataegus oxyacantha and Crataegus monogyna are used in the therapy for moderate (NYHA II) congestive heart failure. 1,2) These extracts exert several pharmacological and clinical effects, such as antiischemia/reperfusion-injury, anti-arrhythmic, hypolipidemic, and hypotensive effects. 1,2) Flavonoids and procyanidins are considered to be the two main active constituents. 2) Species of Chinese hawthorn, particularly Crataegus pinnatifida and Crataegus pinnatifida Bge. var. major NE Br., have also been used as herbal medicines. 2,3) Studies indicate that Chinese hawthorn extracts have beneficial effects, such as antioxidant, 4,5) anti-inflammatory, 6) and hypolipidemic effects, 7) as well as protective effects on the brain and vascular endothelium 8,9) and endothelium-dependent relaxation. 10) Recently, we analyzed 37 major cultivars of Chinese hawthorn using high performance liquid chromatography (HPLC), and found that procyanidins, which were present in high concentrations, were the main phenolic active constituents in these fruits. 11) In general, the hawthorn found in China is an edible fruit that is popular for its special acidic taste. It is bigger than its European counterpart. Since the content of organic acid in Chinese hawthorn fruits can be up to 5% (fresh weight), they are used to produce various foods, such as jams, jellies, juices, tinned foods, wines, and various sweet foods. However, the high sugar content of these fruits counteracts the benefits of polyphenols for consumers of advanced age or for those who suffer from hypertension, cardiovascular disease, or diabetes. Hence, it is desirable to separate the polyphenol and sugar components. Previously, hawthorn extract (HE) for medical applications were ethanol-extracted from the dried fruit of the Chinese hawthorn. 12) These crude extracts contain many undesirable substances, such as carbohydrates, organic acids, and moisture, which not only reduce the concentration of acti...

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Section: Contents Of Organic Acid and Polyphenolssupporting

confidence: 75%

Section: Antioxidant Activities Of Hawthorn Extracts and Related Compmentioning

confidence: 99%

Polyphenolic Content and Physiological Activities of Chinese Hawthorn Extracts

Cui

Nakamura

Tian

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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“…They found that the scavenging activities of the cell suspension extracts were similar to those of the flowers. Furthermore, some reports studied the antioxidant actions of Crataegus monogyna callus cultures and showed that a high TEAC values were observed when the culture medium contained 2.0 mg/L 2,4-D and 0.5 mg/L KIN, these values were strongly influenced by the total flavonoids content (Bahorun et al 1994(Bahorun et al , 2003. In addition, Bahri-Sahloul et al showed a high DPPH radical scavenging effect in Salvia officinalis shoot cultures grown on MS medium containing a high ratio of BAP over IAA which was comparable to that of collected plants.…”

Section: Discussionmentioning

confidence: 99%

Effects of 2,4-dichlorophenoxyacetic acid combined to 6-Benzylaminopurine on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leaf tissue cultures of Crataegus azarolus L. var. aronia

et al. 2015

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The present research work describes the effects of 2,4-dichlorophenoxyacetic acid (2,4-D)/Ben-zylaminopurine (BAP) ratio on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leafderived calli of Crataegus azarolus (hawthorn). The supplementation of 1.0 mg/L 2,4-D and 1.0 mg/L BAP to MS medium was found to be the most efficient for callus induction (as percentage and fresh weigh). The results of biochemical analysis showed that the highest total phenolic contents were obtained in callus cultured on MS medium supplemented with 2.0 mg/L 2,4-D and 1.0 mg/L BAP (52 ± 0.56 mg GAE/g DM) and were significantly lower than those of intact leaves (76±1.72mg GAE/g DM). However, the highest ascorbic acid contents were found in callus cultured on MS medium supplemented with 1.0 mg/L 2,4-D and 0.5 mg/L BAP (0.96 ± 0.13 mg AAE/g DM) and these amounts were statistically similar to those found in leaf tissues (0.74 ± 0.07 mg AAE/g DM). Antioxidant activities of callus extracts were determined using two TEAC assays and results showed that extract of callus cultured on MS medium supplemented with 2.0 mg/L 2,4-D and 1.0 mg/L BAP have the greatest antiradical activities against DPPH (124 ± 2.92 mg TE/g DM) and ABTS (0.19 ± 0.02 mg TE/g DM) compared to the leaves of field-grown plant. Thus, the use of high level of 2,4-D over BAP can be suitable to enhance the quality more than the quantity of bioactive compounds in leaf callus culture of hawthorn.

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“…Crataegus may have cardio protective properties (Chatterjee et al, 1997). Antioxidant activity of hawthorn species has already been reported (Bahorun et al, 1994, Ljubuncic et al, 2005. Hawthorn contains different phenolic and flavonoids compounds, including: chlorogenic acid, epicatechin, rutin, hyperoside and vitexin (Zhang et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Antihypoxic activities of Crataegus pentaegyn and Crataegus microphylla fruits-an in vivo assay

Ebrahimzadeh

Khalili

Jafari

et al. 2018

Braz. J. Pharm. Sci.

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The aim of this study was to evaluate anti-hypoxia activity of polyphenolic extracts of Crataegus microphylla and Crataegus pentaegyn on mice. Three experimental models of hypoxia were considered, including asphyctic hypoxia, haemic hypoxia, and circulatory hypoxia. Polyphenolic extract of both plants exhibited significant anti-hypoxic activity and prolonged animal survival time. Anti-hypoia activity of C. pentaegyn was found to be superior to that of C. microphylla in circulatory and asphyctic hypoxia. Antihypoxic activity of these extracts may be attributed to their phenolic compounds.

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Antioxidant Activities ofCrataegus monogynaExtracts

Cited by 106 publications

References 9 publications

Polyphenolic Content and Physiological Activities of Chinese Hawthorn Extracts

Polyphenolic Content and Physiological Activities of Chinese Hawthorn Extracts

Effects of 2,4-dichlorophenoxyacetic acid combined to 6-Benzylaminopurine on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leaf tissue cultures of Crataegus azarolus L. var. aronia

Antihypoxic activities of Crataegus pentaegyn and Crataegus microphylla fruits-an in vivo assay

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