Antioxidant activities of flavonol derivatives from the leaves and stem bark of Scutia buxifolia Reiss

Boligon, Aline Augusti; Pereira, Romaiana Picada; Feltrin, Andriéli Cassel; Machado, Michel Mansur; Janovik, Vanessa; Rocha, João Batista Teixeira da; Athayde, Margareth Linde

doi:10.1016/j.biortech.2009.03.091

Cited by 96 publications

(89 citation statements)

References 43 publications

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“…The IC 50 values of extracts and fractions of leaves were significantly lower than stem in DPPH free radicals assay. The results were not fully agreed with Boligon's study (Boligon et al 2009), in which very good antioxidant activities were found for the butanolic and ethyl acetate fractions from both stem bark and leaves, with similar antioxidant profiles. In addition, the considerable differences might depend on the distinct distribution of the antioxidant constituents in which the plant has biosynthesized.…”

Section: Dpph Radical Scavenging Activitycontrasting

confidence: 75%

Comparative studies on antioxidant activities of extracts and fractions from the leaves and stem of Epimedium koreanum Nakai

et al. 2011

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The antioxidant potency of various extracts and fractions from the leaves and stem of Epimedium koreanum Nakai was evaluated using three esteblished methods, specifically the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radica-scavenging activity assay, the inhibitory effect on lipid peroxidation induced by Fe 2+ /ascorbate (MDA) assay and the ferric reducing power (FRP) assay. The amounts of total phenolics and total flavonoids in the extracts and fractions were determined by spectrophotometric methods and the content of icariin was determined by HPLC. The results showed that all the extracts and fractions exhibited antioxidant activities at different magnitudes of potency. The leaf extract and fractions demonstrated superior antioxidant activity in most of the assays. The decreasing order of antioxidant activities among the extracts/fractions assayed through the three methods were found to be n-BuOH fraction>ethyl acetate fraction>ethanol extract>petroleum ether fraction>-water fraction. A positive correlation was found between the amounts of total phenolics, total flavonoids and icariin and DPPH radical scavenging activity (R 2 =0.9935, 0.9944 and 0.9997, respectively) and inhibitory activity on lipid peroxidation (R 2 =0.9987, 0.9830 and 0.9886, respectively). The results suggested that Icariin was one of the main constituents contribute to the antioxidant activity of Epimedium koreanum Nakai and the n-BuOH fractions of leaf extract might be valuable antioxidant natural sources.

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Section: Dpph Radical Scavenging Activitycontrasting

confidence: 75%

Comparative studies on antioxidant activities of extracts and fractions from the leaves and stem of Epimedium koreanum Nakai

et al. 2011

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“…The essential oil showed only moderate activity against S. aureus and Micrococcus sp. (MIC = 500 μg/mL) and E. coli (250 μg/mL), previous study describes the activity of S. buxifolia against S. aureus (Boligon et al 2009). Sesquiterpenoids spathulenol, β-cubebene, germacrene D and carvacrol were the main components identified in this essential oil and may be responsible, in part, for the antimicrobial activity described, since spathulenol (Chinou et al 2004) and carvacrol (Burt 2004) have been reported to present notable antimicrobial activity against bacterial infections.…”

Section: Resultsmentioning

confidence: 99%

“…Ethanol was used to calibrate the spectrophotometer. The test was performed in triplicate and the calculation of the antioxidant activity followed the equation: % Inhibition = [(A 0 -A 1 )/A 0 ] x 100, where A 0 was the absorbance of the control sample (without essential oil) and A 1 was the absorbance in the presence of the sample (Boligon et al 2009 (1000, 750, 500, 250, 125, and 62.5 µg/ mL) were prepared. The first dilution was made in DMSO and further dilutions in the culture medium.…”

Section: Quantitative Analysis Of Antioxidant Activitymentioning

confidence: 99%

“…In these regions, an aqueous infusion prepared with stem bark and leaves of Scutia buxifolia has been described and widely used in folk medicine for cardiotonic, diuretic and antihypertensive purposes (Boligon et al 2009, Da Silva et al 2012. Phytochemical screening of fractions of Scutia buxifolia bark revealed the presence of cyclopeptide alkaloids (Maldaner et al 2011), polyphenols and flavonoids in fractions of Scutia buxifolia leaves and stem bark (Boligon et al 2009(Boligon et al , 2012a.…”

Section: Introductionmentioning

confidence: 99%

“…Cytotoxicity of extracts from leaves, twigs and stem bark of the plant were evaluated by the Artemia salina assay, as well as the antimicrobial activity against a panel of microorganism strains (Boligon et al 2012b). Extracts of the leaves and stem bark of S. buxifolia were effective against lipid peroxidation by inhibiting the production of thiobarbituric acid reactive substances and also presented DPPH scavenger activity (Boligon et al 2009). …”

Section: Introductionmentioning

confidence: 99%

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Scutia buxifolia Reissek essential oil: in vitro antioxidant and antimicrobial activities

Boligon

Piana

Brum

et al. 2014

An. Acad. Bras. Ciênc.

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The volatile oil from the stem bark of Scutia buxifolia (Rhamnaceae) has been obtained by hydrodistillation and analyzed by GC-MS. Twenty-one components were identified representing 99.93 % of the total oil composition, spathulenol (35.87%), β-cubebene (17.26%), germacrene D (6.43%), linalool (5.19%), carvacrol (4.05%) were the main components of S. buxifolia essential oil. Antioxidant and antimicrobial properties of the essential oil were evaluated by free radical scavenging (DPPH) assay and micro broth dilution method, respectively. S. buxifolia essential oil presented interesting radical scavenging activity (IC 50 = 15.03 ± 0.11 μg/mL). The antibacterial assay showed that S. buxifolia stem bark essential oil was moderately active against the Staphylococcus aureus and Micrococcus sp. (MIC = 500 μg/mL) and Escherichia coli (250 μg/mL). To the best of our knowledge, this is the first study on the composition, antioxidant and antimicrobial activities of essential oil from the S. buxifolia collected from Brazil.

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Gallic acid functions as a TRPA1 antagonist with relevant antinociceptive and antiedematogenic effects in mice

Trevisan

Rossato

Tonello

et al. 2014

Naunyn-Schmiedeberg's Arch Pharmacol

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The transient receptor potential ankyrin 1 (TRPA1) has been identified as a relevant target for the development of novel analgesics. Gallic acid (GA) is a polyphenolic compound commonly found in green tea and various berries and possesses a wide range of biological activities. The goal of this study was to identify GA as a TRPA1 antagonist and observe its antinociceptive effects in different pain models. First, we evaluated the ability of GA to affect cinnamaldehyde-induced calcium influx. Then, we observed the antinociceptive and antiedematogenic effects of GA (3-100 mg/kg) oral administration after the intraplantar (i.pl.) injection of TRPA1 agonists (allyl isothiocyanate, cinnamaldehyde, or hydrogen peroxide-H2O2) in either an inflammatory pain model (carrageenan i.pl. injection) or a neuropathic pain model (chronic constriction injury) in male Swiss mice (25-35 g). GA reduced the calcium influx mediated by TRPA1 activation. Moreover, the oral administration of GA decreased the spontaneous nociception triggered by allyl isothiocyanate, cinnamaldehyde, and H2O2. Carrageenan-induced allodynia and edema were largely reduced by the pretreatment with GA. Moreover, the administration of GA was also capable of decreasing cold and mechanical allodynia in a neuropathic pain model. Finally, GA was absorbed after oral administration and did not produce any detectable side effects. In conclusion, we found that GA is a TRPA1 antagonist with antinociceptive properties in relevant models of clinical pain without detectable side effects, which makes it a good candidate for the treatment of painful conditions.

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Antioxidant activities of flavonol derivatives from the leaves and stem bark of Scutia buxifolia Reiss

Cited by 96 publications

References 43 publications

Comparative studies on antioxidant activities of extracts and fractions from the leaves and stem of Epimedium koreanum Nakai

Comparative studies on antioxidant activities of extracts and fractions from the leaves and stem of Epimedium koreanum Nakai

Scutia buxifolia Reissek essential oil: in vitro antioxidant and antimicrobial activities

Gallic acid functions as a TRPA1 antagonist with relevant antinociceptive and antiedematogenic effects in mice

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