Antioxidant Activities of Extract Fractions of Leaves from Loquat (Eriobotrya japonica Lindl.) by Cultivars

Abstract

In this study, we compared the antioxidant activities of three loquat (Eriobotrya japonica Lindl.) by cultivars (Daebang, Bubang and native cultivar). The leaves were extracted by 80% ethanol and then fractionated with various solvents (n-hexane, ethyl acetate, n-butanol, and water) for biological activities and bioactive compounds analysis. Total polyphenol content of extracts and fractions was in the range of 84.93～478.50 mg/g gallic acid equivalent (GAE). From highest to lowest GAE, the fractions were n-butanol> ethyl acetate> n-hexane> 80% ethanol> water. Among the three cultivars, the highest polyphenol content was found from native cultivar. As for DPPH radical scavenging activity, the n-butanol fraction showed the highest activity, and native cultivar was the highest on the IC50 values (0.18 mg/mL). In the nitrite scavenging activity, the ethyl acetate fraction (54.99～60.86%) showed the most effective activity on the Bubang cultivar was higher than others. The ursolic acid content of the ethyl acetate fraction showed the highest (51.41 mg/g) in the Daebang cultivar. Based on all these results, the Bubang cultivar showed relatively higher antioxidant and nitrite scavenging activities, but the ursolic acid content was higher in the Daebang cultivar. These results suggest that extracts from loquats (Eriobotrya japonica Lindl.) can be used as bioactive and functional materials that could be important information for industrial use in the future.

Developed validation for simultaneous determination of three Di-caffeoylquinic acid derivatives from the Leaf of Eribotrya japonica Lindl. by HPLC-DAD

“…Also, this plant has been reported to have anti-inflammatory, antioxidant and antiviral effects (Bucak et al, 2019;Naveed et al, 2018). Especially, chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid has been focused on regulate fat metabolism and glucose with antioxidant activity and various physiological activities (Khanduja et al, 1999;Lee et al, 2004;Shin et al, 2012;Naveed et al, 2018). The current study was developed a method for the extraction, isolation, and identification of three phenolic compounds from E. japonica leaves.…”

“…Especially, the leaves of E. japonica have been widely used as a traditional medicine with beneficial effects as an expectorant, for hemoptysis, quenching thirst, chronic bronchitis, edema, septic settlement, and a strong stomach (Shin et al, 2012). According to recent study, it has been reported to be the effect of various component analyses (Bae et al, 2002;Bae et al, 2005), gastroenteritis improvement (Takuma et al, 2008), antioxidant (Hwang et al, 2010;Park et al, 2008;Jeong et al, 2009), anticancer (Kim et al, 2009c;Lee et al, 2004), antibacterial (Bae et al, 2002;Bae et al, 2005;, anti-inflammatory (Shin et al, 2012;Kim et al, 2009a;Banno et al, 2005), anti-diabetic (Kim et al, 2009b;Kim et al, 2006;Chen et al, 2008), skin whitening and skin allergy inhibition (Sun et al, 2007). Also, it has garnered attention in various herbal ingredients markets because it is safe and does not have side effects (Hwang et al, 2010;Lee et al, 2004).…”

Correlation of the free radical and antioxidant activities of Eriobotrya Japonica Lindl. with phenolic and flavonoid contents

“…It is grown mainly in mild climatic conditions such as Jeju, Gyeongnam and Jeonnam in Korea (Park & Park, 1995;Park & Kim, 2000). It has been known in traditional medicines that E. japonica fruits and leaves are effective for antitussives, expectorant, vomiting, hemoptysis, and diuresis, and are also reported to be effective in relieving breathing and thirst (Eom et al, 2009;Shin et al, 2012;Lee et al, 2016a). The E. japonica leaves contains a large amount of bioactive compounds such as terpenoid-based compounds (ursolic acid, oleanolic acid etc.)…”