Antioxidant activities of different solvent extracts of Piper retrofractum Vahl. using DPPH assay

Jadid, Nurul; Hidayati, Dewi; Hartanti, Sylviana Rosyda; Arraniry, Byan Arasyi; Rachman, Rizka Yuanita; Wikanta, Wiwi

doi:10.1063/1.4985410

Cited by 73 publications

(58 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…DPPH assay is a fast and efficient method to determine the free radical scavenging activity. The color changed from purple to yellow signifies the decline in absorbance of the DPPH radical (Jadid et al, 2017). In this study, all extracts showed a similar increasing trend in antioxidant activity with an increase in their concentrations (Table 9).…”

Section: Gc-ms Analysissupporting

confidence: 62%

“…In this study, all extracts showed a similar increasing trend in antioxidant activity with an increase in their concentrations (Table 9). IC means inhibition concentration and IC 50 is the concentration of the sample or antioxidant that is required to inhibit 50% of DPPH radicals (Jadid et al, 2017). Hence, the lower the IC 50 , the better the antioxidant activity.…”

Section: Gc-ms Analysismentioning

confidence: 99%

See 1 more Smart Citation

Preliminary Phytochemical Screening, Gc-MS Profiling and in Vitro Evaluation of Biological Activities of Garcinia Atroviridis Root Extracts.

SalsabilaAhmadRoslan¹,

Zareen²,

Zamri³

et al. 2018

IJAR

View full text Add to dashboard Cite

Section: Gc-ms Analysissupporting

confidence: 62%

Section: Gc-ms Analysismentioning

confidence: 99%

Preliminary Phytochemical Screening, Gc-MS Profiling and in Vitro Evaluation of Biological Activities of Garcinia Atroviridis Root Extracts.

SalsabilaAhmadRoslan¹,

Zareen²,

Zamri³

et al. 2018

IJAR

View full text Add to dashboard Cite

“…The differences may be the result of many factors, including the different varieties of the flowers, genetics, the environment of the plant; the choice of solvent; and the solubility of the product [1]. Ethyl acetate is typically used to extract terpenoids, flavonoids, and alkaloids, while ethanol is typically used to extract polyphenols, saponins, flavanols, and alkaloids [10].…”

Section: Fig 1: the Relationship Between The Percentage Of Inhibitiomentioning

confidence: 99%

Antioxidative Activity and Phytochemistry Profile of Hibiscus Sabdariffa Herb Extracts

Fitrotunnisa

Arsianti²,

Tejaputri

et al. 2019

Int J App Pharm

View full text Add to dashboard Cite

Objective: Hibiscus sabdariffa, known as Roselle, is a widely-cultivated herb in Indonesia and has been consumed as an herbal drink due to its medicinal properties. The purpose of this research is to identify the antioxidant activity and phytochemical profile of Hibiscus sabdariffa. Methods: The Hibiscus sabdariffa samples were extracted and macerated with three different organic solvents: ethyl acetate, ethanol, and n-hexane. These extracts were then analyzed using thin layer chromatography (TLC) and phytochemical tests to identify the extracts’ secondary metabolites. The extracts’ antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Results: The phytochemistry tests were positive for glycosides, alkaloids, steroids, triterpenoids, tannins, and flavonoids. The TLC analysis revealed that the extracts containing two to three organic compounds. The ethanol Hibiscus sabdariffa extracts with an IC50 value 103.63 ppm showed stronger antioxidant activity than the ethyl acetate extract. Conclusion: Ethanol Hibiscus sabdariffa extracts may be a potential source of natural antioxidant.

show abstract

“…DPPH assay is the fast and reliable method to estimate free radical scavenging activity (Pavithra & Vadivukkarasi, ). DPPH, a stable free radical accepts an electron of hydrogen radical from an antioxidant to form a diamagnetic molecule (Jadid et al, ). An antioxidant can donate an electron to DPPH, thereby reducing the violet color of DPPH to orange.…”

Section: Discussionmentioning

confidence: 99%

Crassocephalum rubens, a leafy vegetable, suppresses oxidative pancreatic and hepatic injury and inhibits key enzymes linked to type 2 diabetes: An ex vivo and in silico study

et al. 2019

View full text Add to dashboard Cite

Crassocephalum rubens falls under the wild edible, under‐cultivated traditional leafy vegetables (TLV) in Africa; it is used by locals in managing diabetes mellitus among other diseases. This study investigated the in vitro, ex vivo antioxidant and antidiabetic potentials of different extracts of C. rubens. The ameliorative effects of the extracts on Fe2+‐induced oxidative injury was investigated ex vivo together with the effects of the aqueous extract on intestinal glucose absorption and muscle glucose uptake in freshly harvested tissues from normal rats. The aqueous extract was subjected to Liquid Chromatography‐Mass Spectrometry (LC‐MS) analysis to identify possible bioactive compounds which were then docked with the tested enzymes through in silico modeling. The extracts exhibited antioxidant activity, inhibited α‐glucosidase and lipase enzyme activities, intestinal glucose absorption and enhanced muscle glucose uptake compared to controls. Sanguisorbic acid dilactone identified through LC‐MS analysis showed a high binding affinity for catalase and lipase enzymes. Practical applications The results of this study suggest that the aqueous extract of C. rubens possesses better antioxidant and possible antidiabetic potentials compared to other extracts which could be associated to the synergistic action of its identified bioactive compounds.

show abstract

Antioxidant activities of different solvent extracts of Piper retrofractum Vahl. using DPPH assay

Cited by 73 publications

References 29 publications

Preliminary Phytochemical Screening, Gc-MS Profiling and in Vitro Evaluation of Biological Activities of Garcinia Atroviridis Root Extracts.

Preliminary Phytochemical Screening, Gc-MS Profiling and in Vitro Evaluation of Biological Activities of Garcinia Atroviridis Root Extracts.

Antioxidative Activity and Phytochemistry Profile of Hibiscus Sabdariffa Herb Extracts

Crassocephalum rubens, a leafy vegetable, suppresses oxidative pancreatic and hepatic injury and inhibits key enzymes linked to type 2 diabetes: An ex vivo and in silico study

Contact Info

Product

Resources

About