Antioxidant activities of agaro-oligosaccharides with different degrees of polymerization in cell-based system

Chen, Haimin; Yan, Xiaojun

doi:10.1016/j.bbagen.2004.11.016

Cited by 103 publications

(54 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that the collapse of MMP (Δae m ) is closely related to apoptosis (37). The increased intracellular H 2 O 2 played an important role in AMA-induced cell death in liver cells (38,39) and A549 human lung cancer cells (14). Likewise, AMA induced MMP (Δae m ) loss in Calu-6 cells.…”

Section: Discussionmentioning

confidence: 99%

The effects of N-acetyl cysteine, buthionine sulfoximine, diethyldithiocarbamate or 3-amino-1,2,4-triazole on antimycin A-treated Calu-6 lung cells in relation to cell growth, reactive oxygen species and glutathione

Park¹

2009

Oncol Rep

View full text Add to dashboard Cite

Abstract. Antimycin A (AMA) inhibits mitochondrial electron transport between cytochrome b and c. We recently demonstrated that AMA inhibits the growth of lung cancer Calu-6 cells and the changes of reactive oxygen species (ROS) and glutathione (GSH) levels affect apoptosis in Calu-6 cells. Here, we examined the effects of N-acetylcysteine (NAC, a well known antioxidant), L-buthionine sulfoximine (BSO, an inhibitor of GSH synthesis), diethyldithiocarbamate (DDC, an inhibitor of Cu, Zn-SOD) or 3-amino-1,2,4-triazole (AT, an inhibitor of catalase) on AMAtreated Calu-6 cells in relation to cell death, ROS and GSH levels. Treatment with AMA induced cell growth inhibition, apoptosis and the loss of mitochondrial membrane potential (MMP) (Δae m ) in Calu-6 cells. While the intracellular ROS level was decreased in 50 μM AMA-treated Calu-6 cells, O 2•-levels among ROS were significantly increased. AMA also induced GSH depletion in Calu-6 cells. Treatment with NAC showed decreasing effect on O 2•-levels in AMA-treated cells preventing apoptosis, MMP (Δae m ) loss and GSH depletion in these cells. BSO significantly increased GSH depletion and apoptosis in AMA-treated cells. While both DDC and AT increased ROS levels in AMA-treated Calu-6 cells, only DDC intensified GSH depletion and apoptosis. BSO and AT increased the ROS level in Calu-6 control cells, but these agents did not induce apoptosis and GSH depletion. In conclusion, our results suggest that GSH depletion rather than ROS level in AMA-treated Calu-6 cells is more tightly related to apoptosis.

show abstract

Section: Discussionmentioning

confidence: 99%

The effects of N-acetyl cysteine, buthionine sulfoximine, diethyldithiocarbamate or 3-amino-1,2,4-triazole on antimycin A-treated Calu-6 lung cells in relation to cell growth, reactive oxygen species and glutathione

Park¹

2009

Oncol Rep

View full text Add to dashboard Cite

show abstract

“…reported similar results for polysaccharides from longan fruit pericarp (PLFP), of which the DPPH radical scavenging activity could be improved by application of various ultrasonic power, time and temperature. According to Chen and Yan (2005), Fig. 2.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Ultrasonic-assisted Extraction Technology of Sargassum fusiforme Polysaccharides and Evaluation of Their Antioxidant Activity

Wang

et al. 2013

FSTR

View full text Add to dashboard Cite

An ultrasonic-assisted extraction method was employed to get high quality bioactive polysaccharides from Sargassum fusiforme (SFP), with the technology optimized in light of an orthogonal test design L 9 (3 3 ). Being the major factors that influence the yield of the extracts, the ultrasonic power, water/solid ratio, and extraction time were selected on the basis of a single-factor test. The extraction yield and the antioxidant activity of SFP were used as dependent variables in the analysis of the orthogonal test results. The antioxidant activity of SFP was evaluated using IC 50 of scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) as the activity monitoring parameter. The optimum extraction conditions were obtained when the ultrasonic power, the extraction time, and the ratio of water to solid were 800 W, 10 min and 20, respectively. Under the optimized conditions, the SFP extracted with the optimized ultrasonic-assisted method exhibited strongest DPPH radical scavenging activity (P < 0.05) compared with conventional hot water extracted SFP and other two antioxidants.Keywords: Sargassum fusiforme, polysaccharides, orthogonal experiment, extraction, antioxidant *To whom correspondence should be addressed. E-mail: qiangy@zwu.edu.cn IntroductionSargassum fusiforme, the brown alga, is one of the most widely consumed seaweeds in China, Japan and Korea, not only as food, but also as herbal medicine. As an important economic alga, it is now cultured on a vast scale in coastal zone in China's Zhejiang and Fujian provinces (Huang et al., 2006). The polysaccharides extracted from Sargassum fusiforme (SFP) in medicine are polymeric carbohydrate structures and have widely been suggested as antioxidants (Siriwardhana et al., 2004), anti-coagulants (Kim et al., 1998, and tumor inhibitor (Han et al., 2009) because of their chemical properties and biological activities (Zhu et al., 2010). However, the utilization of SFP currently is restricted by its polysaccharides extraction technology, which is fundamental for its application or further research and development.The conventional and most common extraction method for polysaccharides is the hot water extraction (HWE) method and many papers have been aimed at investigating the influence of extraction parameters, such as particle size, ratio of solvent to raw material, extraction time, extraction temperature, pH value and number of extraction (Wang et al., 2009a. It should be noted that HWE of polysaccharides is always time-consuming, laborious and polysaccharides losing. To solve these problems, the acid extraction method came into being. This method is not without its flaws, however. It may easily alter the structure of the polysaccharide related immunocompetence (Abdel-Fattah et al., 1974). Therefore, alternative extraction techniques like the microwave-assisted extraction (MAE) and ultrasonic assisted extraction (UAE) technologies have attracted considerable interests and have been employed to extract polysaccharides from different plant materials, including Cyclocarya paliuru...

show abstract

“…It has been reported that the antioxidant activity of oligosaccharides can be affected significantly by the polymerization degree (Chen and Yan 2005). To date, data have revealed that a polymerization degree of six is beneficial to the improvement of antioxidant activity.…”

Section: Antioxidant Activity Of Fos In Vivomentioning

confidence: 99%

Antioxidant Activity In Vivo and In Vitro of Two Feruloyl Oligosaccharides Preparations Produced from Wheat Bran and Fermented by Aureobasidium pullulans

Yu¹,

Zhu²,

Gu³

et al. 2015

BioResources

View full text Add to dashboard Cite

The antioxidant functions of two feruloyl oligosaccharide (FO1 and FO2) were investigated in vivo and in vitro. Effects of FO1 and FO2 on hemolysis of rat red blood cell (RBC) and malondialdehyde (MDA) formation in rat liver homogenate and rat liver mitochondria in vitro were studied. Hemolysis of rat RBC and MDA formation in rat liver homogenate and rat liver mitochondria were inhibited in a dosagedependent manner by FO1 and FO2 in the tested concentration range of 0.5 to 10 mg/mL. The results showed that FO1 and FO2 had antioxidative activity in vitro, and the effect of FO2 was better than that of FO1. With increasing dosage, FO1 and FO2 could increase the activity of SOD and GSH-Px in serum of S180 tumor-bearing mice, reduce the level of MDA, and thus improve the activity of the antioxidant in vivo. When the dosage reached 250 mg/kg/d, FO2 was more likely to improve the capabilities of the antioxidants of tumor-burdened mice than were 5-FU and FO1 in vivo. Thus, these oligosaccharides may be used as functional biological materials produced from fermented lignocellulose of WB.

show abstract

Antioxidant activities of agaro-oligosaccharides with different degrees of polymerization in cell-based system

Cited by 103 publications

References 20 publications

The effects of N-acetyl cysteine, buthionine sulfoximine, diethyldithiocarbamate or 3-amino-1,2,4-triazole on antimycin A-treated Calu-6 lung cells in relation to cell growth, reactive oxygen species and glutathione

The effects of N-acetyl cysteine, buthionine sulfoximine, diethyldithiocarbamate or 3-amino-1,2,4-triazole on antimycin A-treated Calu-6 lung cells in relation to cell growth, reactive oxygen species and glutathione

Optimization of Ultrasonic-assisted Extraction Technology of Sargassum fusiforme Polysaccharides and Evaluation of Their Antioxidant Activity

Antioxidant Activity In Vivo and In Vitro of Two Feruloyl Oligosaccharides Preparations Produced from Wheat Bran and Fermented by Aureobasidium pullulans

Contact Info

Product

Resources

About