Antioxidant activities and simultaneous HPLC-DAD profiling of polyphenolic compounds from Moringa oleifera Lam. Leaves grown in Bangladesh

Abstract

Moringa oleifera Lam. has been used as a traditional medicine for the treatment of several diseases. In this study, the antioxidant activity and simultaneous HPLC profiling of polyphenolic compounds in 80% ethanol and water extracts of M. oleifera tender and mature leaves available in Bangladesh were investigated. The 80% ethanol extract of M. oleifera tender leaves contained high concentration and more amount of 3,4- dihydroxybenzoic acid, (-) epicatechin and rosmarinic acid (201.32±1.94, 213.08±1.96 and 133.84±1.42 mg/100 g of dry extract, respectively) than that of mature leaves (82.55±1.09, 141.86±1.10 and 16.23±0.76 mg/100 g of dry extract, respectively). On the other hand, the water extract of M. oleifera tender leaves contained a high concentration of catechin hydrate, trans-ferulic acid and quercetin (530.05±1.91, 166.38±0.90 and 771.74±1.34 mg/100 g of dry extract, respectively) than that of mature leaves (207.21±0.90, 62.42±0.45 and 483.25±1.04 mg/100 g of dry extract, respectively). It was also found that 3,4-dihydroxybenzoic acid, catechol, p-coumaric acid and trans-cinnamic acid were detected only in 80% ethanol extract of M. oleifera leaves but syringic acid was identified in the water extract. In DPPH free radical scavenging activity test, IC50 values of 80% ethanol extract of tender leaves (EETL), 80% ethanol extract of mature leaves (EEML), water extract of tender leaves (WETL) and water extract of mature leaves (WEML) were found to be moderately significant (105.50±1.05, 115.00±1.14, 216.00±1.34 and 269.50±1.56 μg/mL, respectively) when compared to the IC50 value of ascorbic acid, 3.32±0.07 μg/mL. The polyphenolic compounds are responsible for antioxidant activity. On the basis of the present study, it can be suggested that the leaves show antioxidant activity and it’s contained a significant amount and different types of polyphenolic compounds. Standardized polyphenolic compounds from M. oleifera leaves could be used as a better source of natural antioxidant supplement.

Effects of Drying Temperature and Solvents on In Vitro Diabetic Wound Healing Potential of Moringa oleifera Leaf Extracts

“…Previous research has reported the phytochemicals, namely, kaempferol and quercetin in M. oleifera leaf extracts [ 51 ]. Another study reported the ethanolic extract of M. oleifera contained p-Coumaric acid and quercetin [ 52 ]. Chlorogenic acid, catechin, caffeic acid, rutin, quercetin, and kaempferol were identified in the leaves of M. oleifera using HPLC analysis [ 53 ].…”

Extraction of a novel tanning agent from indigenous plant bark and its application in leather processing

“…X. granatum sample was taken into a 10 mL test tube separately [36]. 7.5 mL of de-ionized water and 0.5 mL Folin-Ciocalteu reagent (without dilution) were added to each tube followed by the addition of 1.0 mL of 35% (w/v) sodium carbonate.…”

“…Then automatic baseline correction and normalization were carried out by the FTIR built-in software. HPLC-DAD analysis was performed for the detection and quantification of polyphenolic compounds in methanol (100%) extracted X. granatum, mimosa tannin (standard), and quebracho tannin (standard) as delineated by Ahmed [36] with some modifications. HPLC instrument (Shimadzu LC-20A, Japan) consisting of the binary solvent delivery pump (LC-20AT), auto-sampler (SIL-20A HT), column oven (CTO-20A), a detector of photodiode array (SPD-M20A) was used and run by the LC solution software for analysis.…”

Analgesic and anthelmintic activities in common fruits of the Sundarbans mangrove forest, Bangladesh

“…where n = number of treatment; i = initial treatment number. Selected phenolic compounds in the extract of C. decandra were detected and quantified using HPLC-diode array detector (DAD) analysis (Ahmed et al 2021).…”