Antioxidant Action of Ergothioneine: Assessment of Its Ability to Scavenge Peroxynitrite

Aruoma, Okezie I.; Whiteman, Matthew; England, Timothy G.; Halliwell, Barry

doi:10.1006/bbrc.1997.6109

Cited by 120 publications

(95 citation statements)

References 37 publications

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“…SEM were obtained from three replicate plates. DOI: 10.3109/13880209.2013.765897 activity can accelerate damage to molecules such as DNA, carbohydrates, or proteins (Aruoma et al, 1997). We have previously demonstrated that both ascorbic acid and b-carotene induce concentration and time-dependent genotoxic and cytotoxic damage on HepG2 human hepatocellular carcinoma cells together with increased oxidative damage at their relevant achievable plasma level concentrations (Yurtcu et al, 2011) in contrast to their protective effect on lymphocytes (Yurtcu et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Corchorus olitorius(jute) extract induced cytotoxicity and genotoxicity on human multiple myeloma cells (ARH-77)

İşeri

Yurtçu²,

Şahin

et al. 2013

Pharmaceutical Biology

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Context: Corchorus olitorius L. (Malvaceae) has industrial importance in world jute production and is a widely cultivated and consumed crop in Cyprus and in some Arabic countries. Objective: The present study investigated cytotoxic and genotoxic effects of leaf extracts (LE) and seed extracts (SE) of the C. olitorius on the multiple myeloma-derived ARH-77 cells. The extracts were also evaluated for their total phenol content (TPC) and free radical scavenging activity (FRSA). Materials and methods: C. olitorius was collected from Nicosia, Cyprus. TPC and FRSA were measured by Folin-Ciocalteu and DPPH free radical methods, respectively. Cytotoxicity was evaluated by the MTT assay (4-2048 mg/mL range), and DNA damage (at IC 50 and ½IC 50 ) was measured by the comet assay. Results and discussion: The LE had significantly higher total phenol (78 mg GAE/g extract) than the SE (2 mg GAE/g extract) with significantly higher FRSA (IC 50 LE: 23 mg/mL and IC 50 SE: 10 401 mg/mL). Both LE and SE exerted cytotoxic effects on cells after 48 h. The IC 50 of SE (17 mg/mL) was lower than LE (151 mg/mL), which demonstrates its higher cytotoxicity on cells. The extracts were applied at 150 and 75 mg/mL for LE and at 17 and 8.5 mg/mL for SE, and the results of the comet assay revealed that the extracts induced genotoxic damage on ARH-77 cells. In both 48 h leaf and seed extract treatments, genotoxic damage significantly increased with increasing concentrations at relevant cytotoxic concentrations. Conclusion: To our knowledge, this is the first report demonstrating the high cytotoxic potential of C. olitorius SE and the genotoxic potential of LE and SE.

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Section: Discussionmentioning

confidence: 99%

Corchorus olitorius(jute) extract induced cytotoxicity and genotoxicity on human multiple myeloma cells (ARH-77)

İşeri

Yurtçu²,

Şahin

et al. 2013

Pharmaceutical Biology

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“…The functional ability to scavenge singlet oxygen, hydroxyl radicals and peroxyl radicals (Akanmu et al, 1991;Aruoma et al, 1997) has already been reported. Colognato et al (2006) and Rahman et al (2003) reported that EGT inhibited both H 2 O 2 -induced cell death and the TNF-α-mediated activation of NF-κB.…”

Section: Isolation and Identification Of Anti-oxidative And Antiinflamentioning

confidence: 95%

Ergothioneine as an Anti-Oxidative/Anti-Inflammatory Component in Several Edible Mushrooms

Ito

Kato

Tsuchida

et al. 2011

FSTR

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We measured the anti-oxidative and anti-inflammatory activities of hot water extracts prepared from 11 species of mushrooms. Anti-oxidative activity was evaluated using the oxygen radical absorbance capacity method, and anti-inflammatory activity was examined by measuring the inhibition of lysine chloramine formation by hypochlorous acid. Hot water extracts of Grifola gargal (G. gargal) showed the strongest anti-oxidative activity and inhibitory effects on lysine chloramines. Hot water extracts of G. gargal were evaluated by HPLC and divided into 8 fractions. The most active fraction among these 8 fractions was further purified by preparative HPLC. The active component isolated by HPLC was identified as ergothioneine (EGT) using spectral analysis. On HPLC analysis, the EGT content in G. gargal was the highest among the 11 species of mushrooms. We also examined the protective role of EGT by examining the inflammatory response of adipocyte cells induced by tumor necrosis factor-α.

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“…Ergothioneine also interacts with ROS, most probably through the quenching of singlet oxygen (Hartman et al, 1988). In addition, the compound reacts with hydroxyl radicals (Rougee et al, 1988;Akanmu et al, 1991), and scavenges hypochlorous acid (Akanmu et al, 1991), peroxyl radical (Asmus et al, 1996), and peroxynitrite (Aruoma et al, 1997). The effects of ergothioneine on oxidative and/or antioxidant enzymes are largely unexplored.…”

Section: Ergothioneine Protects Endothelial Cellsmentioning

confidence: 99%

“…Ergothioneine was first identified as an antioxidant in cell-free experiments. It inhibits formation of hydroxyl radical (Motohashi and Mori, 1986), and superoxide anions (Cargnoni et al, 1995), singlet oxygen at ASPET Journals on May 11, 2018 jpet.aspetjournals.org production (Obayashi et al, 2005), lipid peroxidation (Deiana et al, 2004), and peroxynitrite oxidative damage (Aruoma et al, 1997). As opposed to other naturally occurring thiols (e.g., glutathione, N-acetylcysteine), ergothioneine is present in aqueous solutions predominantly as a thione rather than a tautomeric thiol structure.…”

Section: Introductionmentioning

confidence: 99%

Uptake and Protective Effects of Ergothioneine in Human Endothelial Cells

Yang²,

Sit

et al. 2014

J Pharmacol Exp Ther

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Ergothioneine is a thiourea derivative of histidine found in food, especially mushrooms. Experiments in cell-free systems and chemical assays identified this compound as a powerful antioxidant. Experiments were designed to test the ability of endothelial cells to take up ergothioneine and hence benefit from protection against oxidative stress. Reverse-transcription polymerase chain reaction and Western blotting demonstrated transcription and translation of an ergothioneine transporter in human brain microvascular endothelial cells (HBMECs). Uptake of [3 H]ergothioneine occurred by the organic cation transporter novel type-1 (OCTN-1), was sodium-dependent, and was reduced when expression of OCTN-1 was silenced by small interfering RNA (siRNA). The effect of ergothioneine on the production of reactive oxygen species (ROS) in HBMECs was measured using dichlorodihydrofluorescein and lucigenin, and the effect on cell viability was studied using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. ROS production and cell death induced by pyrogallol, xanthine oxidase plus xanthine, and high glucose were suppressed by ergothioneine. The antioxidant and cytoprotective effects of ergothioneine were abolished when OCTN-1 was silenced using siRNA. The expression of NADPH oxidase 1 was decreased, and those of glutathione reductase, catalase, and superoxide dismutase enhanced by the compound. In isolated rat basilar arteries, ergothioneine attenuated the reduction in acetylcholine-induced relaxation caused by pyrogallol, xanthine oxidase plus xanthine, or incubation in high glucose. Chronic treatment with the compound improved the response to acetylcholine in arteries of rats with streptozotocin-induced diabetes. In summary, ergothioneine is taken up by endothelial cells via OCTN-1, where the compound then protects against oxidative stress, curtailing endothelial dysfunction.

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Antioxidant Action of Ergothioneine: Assessment of Its Ability to Scavenge Peroxynitrite

Cited by 120 publications

References 37 publications

Corchorus olitorius(jute) extract induced cytotoxicity and genotoxicity on human multiple myeloma cells (ARH-77)

Corchorus olitorius(jute) extract induced cytotoxicity and genotoxicity on human multiple myeloma cells (ARH-77)

Ergothioneine as an Anti-Oxidative/Anti-Inflammatory Component in Several Edible Mushrooms

Uptake and Protective Effects of Ergothioneine in Human Endothelial Cells

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