Antiosteoporosis Effects of 70% Ethanolic Extract Combination of Dayak Onion Bulbs (Eleutherine bulbosa (Mill.) Urb) and Cowpea (Vigna unguiculata (L.) Walp.) on the Hypoestrogen Rats

Abstract

Introduction: Cowpea and Dayak onion bulbs extracts were proven to have an effect on bone, cause by the content of daidzein and eleutherinol which has been known to have an affinity to bind with estrogen receptor selectively. The objective of this study to determine the effects of Dayak onion bulbs and cowpea extract combination on the level of bone calcium, bone weight, and the scoring of bone marrow fat in the hypoestrogen rats compared with the single dose of cowpea extract. Material and Methods: This study used 32 female, white Sprague-Dawley rats were divided into eight groups. The SHAM group was given CMC 0.5%; the negative group was given CMC 0.5%, the positive group was given raloxifene 1.08 mg/200 g BW, cowpea group was given cowpea extract 100 mg/200 BW. The dose variation was given 70% ethanolic extract combination of Dayak onion bulbs and cowpea with four doses variation 36 mg/200 g; 100 mg/200 g BW, 18 mg/200 g; 100 mg/200 g BW, 9 mg/200 g; 100 mg/ 200 g BW, and 4.5 mg/200 g; 100 mg/200 g BW. Ovariectomy was performed on all groups except the SHAM to obtain the condition of hypoestrogen. Four weeks after the surgery, the rats were treated with an oral administration of extract daily for 28 days. Determination of the levels of tibia bone calcium, tibia bone weight, and the scoring of tibia bone marrow fat counted on day 29. Results: The result showed that Dayak onion bulbs and cowpea extract combination were able to elevate the level of bone calcium and bone weight significantly, and reduced the scoring of bone marrow fat higher than a single dose of cowpea extract. Conclusions: The results showed that Dayak onion bulbs and cowpea extract combination could be developed to be a drug for osteoporosis in the future.

Effects of Eleutherine bulbosa (mill.) urb. bulb extract on mice glucocorticoid-induced osteoporosis models

“…E. bulbosa bulbs are reported to contain 2,4,7-Trihydroxy-9,10-dihydrophenanthrene (phenanthrene), cuspidatumin A (naphthoquinone), dendromoniliside E (glycoside), liquiritigenin (flavonoid), and natsudaidain (flavonoid). 29 In vitro studies have shown that liquiritigenin raises osteoblast activity and reduces osteoclast differentiation. 30 Furthermore, fish scales’ natural bone metabolism can be preserved by liquiritigenin.…”

Eleutherine bulbosa (Mill.) Urb. Bulb: Review of the Pharmacological Activities and Its Prospects for Application

“…Weniger et al [10] reported that the bulb of E. bulbosa displayed anti-fertility potential in the mouse. Bahtiar and Dewi [89] documented that the synergistic effect of E. bulbosa bulb and cowpea extract significantly elevated the calcium level and weight of the bone in ovariectomized rats. The combination of the extracts could also be able to alleviate the level of fat in the bone marrow of the rats, suggesting that a potential application could be further extrapolated in osteoporosis management in post-menopausal women.…”

Sistem Pakar Bawang Dayak Sebagai Obat Alternatif

“…Kandungan senyawa aktif flavonoid pada bawang dayak berperan dalam mengahambat pembentukan Kristal oksalat pada persendian (J.A. Widians, Puspitasari, & Febriansyah, 2019) , (Bahtiar & Dewi, 2019).…”

“…Penyakit rematik ada empat macam, yaitu osteoarthritis, arthritis rheumatoid, polimialgia rheumatoid, dan arthritis gout. Kandungan zat aktif seperti flavonoid dan tannin dalam bawang dayak berfungsi sebagai pereda rematik (Bahtiar & Dewi, 2019).…”