Antiobesity and Antidiabetic Effects of Portulaca oleracea Powder Intake in High-Fat Diet-Induced Obese C57BL/6 Mice

Abstract

This study investigated the hypothesis that Portulaca oleracea L. exerts antiobesity and antidiabetic effects by evaluating blood lipid profiles, blood glucose control factors, protein expression of lipid metabolism, and insulin sensitivity improvement. Three groups of high-fat diet (HFD) induced obese C57BL/6 mice (n = 8) received treatment with low (5%; HFD + PO5%) or high (10%; HFD + PO10%) concentrations of P. oleracea powder for 12 weeks or no treatment (HFD) and were compared with each other and a fourth control group. Weight gain was reduced by 34% in the HFD + PO10% group compared to the HFD group. Moreover, the perirenal and epididymal fat contents in the HFD + PO10% group were 6.3-fold and 1.5-fold, respectively, lower than those in the HFD group. The atherogenic index (AI) and cardiac risk factor (CRF) results in the P. oleracea-treated groups were significantly lower than those in the HFD group. The homeostasis model assessment of insulin resistance (HOMA-IR) levels was lower in the HFD + PO10% group than in the HFD group. The protein expression levels of the proliferator-activated receptor (PPAR)-α, glucose transporter (GLUT) 4 and PPAR-γ were upregulated in the HFD + PO10% group compared to the HFD group. However, the protein expression levels of tumor necrosis factor (TNF)-α were lower in the P. oleracea-treated groups than in the HFD group. Our results demonstrate that P. oleracea powder could be effectively used to treat and prevent obesity and diabetes-associated diseases through suppression of weight gain and reduction in body fat and blood glucose levels.

Similarities and differences in constipation phenotypes between Lep knockout mice and high fat diet-induced obesity mice

“…A similar pattern was observed in the HFD-treated mice. After HFD feeding for 12 weeks, the blood glucose and serum insulin concentrations were 130-160 mg/dL and 27-39 μU/mL, respectively [32][33][34]. In this study, the Lep KO and HFD-treated mice were used to compare the phenotypes of constipation.…”

Potential Roles of Purslane (Portulaca oleracea L.) as Antimetabolic Syndrome: A Review

“…In addition, these antioxidant compounds can also maintain the organ health due to their antimetabolic activity. 22,27,43 The presence of antioxidants in P. olaracea is associated with cellular oxidative stress. Oxidative stress is caused by reactive oxygen species that trigger cell and tissue damage, especially liver tissue.…”

“…18,50 Moreover, purslane extract is known to inhibit reactive oxygen species; decrease adipogenic activity involving the protein PPAR-γ, C/EBPα, FABP4, FATP1 and ACS1, and also acts as an anti-inflammatory agent by reducing TNF-α (tumor necrosis factor α). 3,18,43 As a result, there was a decrease in TC, TG, LDL-C (low density lipoprotein-cholesterol), VLDL-C (very low density lipoproteincholesterol), LPO (lipid peroxidation), AI (anthrogenic index), CRF (cardiac risk factor), CRI (coronary risk index), GOT (glutamateoxaloacetate transaminase), GPT (glutamate pyruvate transaminase), GSH (glutathione), HOMA-IR (homeostatic model assessment of insulin resistance), FBS (fasting blood sugar), and QUICKI (quantitative insulin sensitivity check index) compared with untreated obese mice. 7,22,43,44 Concurrently with this decrease, there was an increase in HDL-C (high density lipoprotein-cholesterol) which resulted in a decrease in body fat and weight loss.…”

Antibacterial diarrhea effect and action mechanism of Portulaca oleracea L. water extract based on the regulation of gut microbiota and fecal metabolism

“…Besides, P. oleracea also provides nutritional benefits due to its rich omega-3 fatty acids and antioxidant properties. 7 Modern pharmacological studies have shown that P. oleracea possesses a variety of bioactivities, including antibacterial, 8 antiulcerogenic, anti-inflammatory, 9 antioxidant, 10 neuroprotective, 11 antidiabetic, 12 antioxidant, 13 anticancer, 14 antimicrobial, 15 antiinflammatory, 16,17 antiulcerogenic, 18 hepatoprotective activity, 19 and wound-healing 20 properties. Besides, there are many compounds, including flavonoids, alkaloids, fatty acids, terpenoids, polysaccharides, vitamins, sterols, proteins and minerals, that have been isolated from P. oleracea.…”