Antinuclear Antibodies Detected by Indirect Immunofluorescence on HEp2 Cells and by Immunoblotting in Patients with Systemic Sclerosis

Briolay, Jérôme; Gioud, M; Monier, J. C.; Rodde, J. L.; Cohen, Jacques H. M.; Roux, B.; Kaliss, B.

doi:10.3109/08916938909019953

Cited by 14 publications

(5 citation statements)

References 32 publications

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“…In general, if a patient presents clinical manifestations of an autoimmune disease, the first test to be requested is ANA detection by indirect immunofluorescence using HEp-2 cells, due to its great sensitivity [1] , [3] . The different possible patterns, the intensity, and the titers obtained by consecutive dilutions must be carefully examined.…”

Section: Introductionmentioning

confidence: 99%

Predictive value of antinuclear antibodies in autoimmune diseases classified by clinical criteria: Analytical study in a specialized health institute, one year follow-up

Soto

Hernández-Becerril

Perez-Chiney

et al. 2015

Results in Immunology

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Introduction: Determination of antinuclear antibodies (ANA) by indirect immunofluorescence (IIF) is usually the initial test for the diagnosis of systemic rheumatic diseases (SRD). Assigning predictive values to positive and negative results of the test is vital because lack of knowledge about ANAs and their usefulness in classification criteria of SRD leads to inappropriate use. Methods: Retrospective study, ANA tests requested by different specialties, correlation to patients' final diagnosis. Results: The prevalence of autoimmune disease was relatively low in our population yielding a low PPV and a high NPV for the ANA test. 40% of the patients had no clinical criteria applied prior to test. Coexistence of two or more autoimmune disorders affects prevalence and predictive values. Conclusion: Application of the test after careful evaluation for clinical criteria remarkably improves the positive likelihood ratio for the diagnosis.

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Section: Introductionmentioning

confidence: 99%

Predictive value of antinuclear antibodies in autoimmune diseases classified by clinical criteria: Analytical study in a specialized health institute, one year follow-up

Soto

Hernández-Becerril

Perez-Chiney

et al. 2015

Results in Immunology

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“…Although we did not perform any experiment to identify the same target antigen in the 65 kDa band, we postulate that this band also contains DNA topoisomerase 1. Thus, it has now been well documented for near twenty years that patients with SSc bind to a 70 kDa antigen in Hela cells extracts and that the target antigen of the anti-Scl-70 Abs is a degraded form of topoisomerase 1 (Kumar et al 1988), and it was postulated that reactivities directed towards Hela cells proteins of 63 to 77 kDa molecular weights bound to topoisomerase 1 (Briolay et al 1989).…”

Section: Discussionmentioning

confidence: 99%

IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts

Tamby

Servettaz²,

Tamas³

et al. 2008

BTT

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By using a semi-quantitative immunoblotting technique, we have analyzed serum immunoglobulin G (IgG) reactivities of patients with limited cutaneous systemic sclerosis and anticentromere antibodies, patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies, patients with diffuse systemic sclerosis without antitopoisomerase 1 or anticentromere antibodies and age-and gender-matched healthy controls with normal human skin fi broblasts and HEp-2 cells antigens. Serum IgG reactivities of patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies differed signifi cantly from those of healthy controls or systemic sclerosis patients in other groups for reactivity with fi broblast proteins. IgG from patients with antitopoisomerase 1 antibodies bound to a 90 kDa fi broblast band and to a 100 kDa protein band in a HEp-2 cell protein extract. These two bands were further identifi ed as DNA topoisomerase 1. Our results indicate that IgG from patients with diffuse systemic sclerosis bind DNA topoisomerase 1 in normal human fi broblasts extracts.

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“…Antinuclear antibody (ANA) testing is currently used to diagnose systemic autoimmune rheumatoid disease (SARD). 1 This testing method is an indirect immunofluorescence (IIF) assay to assess binding of antibodies (Abs) to human HEp-2 cells that originated from human laryngeal epithelial carcinoma. The nature of the target antigen detected by SARD-associated autoantibodies differs between diseases; as such, unique reaction patterns are revealed via ANA testing.…”

Section: Introductionmentioning

confidence: 99%

Stratification of Nuclear Homogeneous Patterns on HEp-2 Cells Based on Neutrophil Nuclear Staining

et al. 2021

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Antinuclear antibody (ANA) testing is used to diagnose systemic autoimmune rheumatic disease (SARD). Nuclear homogeneous patterns on ANA-HEp-2 cells can result from anti-double-stranded DNA (dsDNA), anti-nucleosome, anti-histone, anti-Scl-70, or anti-dense fine speckles 70 (DFS70) antibodies (Abs). This study aimed to find a way to discriminate DFS70 Abs from others by way of assessing neutrophil nuclear staining on anti-neutrophil cytoplasmic antibody (ANCA) testing. Nuclear staining on ANCA-neutrophils was assessed to stratify nuclear homogeneous patterns on ANA-HEp-2 cells. Enrolled subjects included (1) young individuals with a dense fine speckled pattern on ANA testing (young non-SARD group, n=71) and patients with (2) systemic lupus erythematosus (SLE group, n=35); (3) rheumatoid arthritis possibly with histone, nucleosome Abs, and others (RA group, n=51); and (4) diffuse systemic sclerosis with Scl-70 Abs (diffuse SSc group, n=19). Negative rates (95% confidence interval) of neutrophil nuclear staining were 97.2% (90.2%–99.7%) in the young non-SARD group, 2.9% (0.1%–14.9%) in the SLE group, 3.9% (0.5%–13.5%) in the RA group, and 47.4% (24.5%–71.1%) in the diffuse SSc group. The negative rate of the young non-SARD group was significantly higher than those of the other groups (all p<0.05). In conclusion, this study suggests that the assessment of nuclear staining on ANCA-neutrophils can help to stratify nuclear homogeneous patterns on ANA-HEp-2 cells and thus to determine whether the ANA pattern is attributed to DFS70 Abs, which can be found in healthy individuals, especially in young individuals.

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Antinuclear Antibodies Detected by Indirect Immunofluorescence on HEp2 Cells and by Immunoblotting in Patients with Systemic Sclerosis

Cited by 14 publications

References 32 publications

Predictive value of antinuclear antibodies in autoimmune diseases classified by clinical criteria: Analytical study in a specialized health institute, one year follow-up

Predictive value of antinuclear antibodies in autoimmune diseases classified by clinical criteria: Analytical study in a specialized health institute, one year follow-up

IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts

Stratification of Nuclear Homogeneous Patterns on HEp-2 Cells Based on Neutrophil Nuclear Staining

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