Antinociceptive Properties of the Hydroalcoholic Extract and the Flavonoid Rutin Obtained from Polygala paniculata L. in Mice

Abstract

The present study examined the antinociceptive effects of a hydroalcoholic extract of Polygala paniculata in chemical and thermal behavioural models of pain in mice. The antinociceptive effects of hydroalcoholic extract was evaluated in chemical (acetic-acid, formalin, capsaicin, cinnamaldehyde and glutamate tests) and thermal (tail-flick and hot-plate test) models of pain or by biting behaviour following intratecal administration of both ionotropic and metabotropic agonists of excitatory amino acids receptors glutamate and cytokines such as interleukin-1 β (IL-1 β) and tumour necrosis factor-α (TNF-α) in mice. When given orally, hydroalcoholic extract (0.001-10 mg/kg), produced potent and dose-dependent inhibition of acetic acid-induced visceral pain. In the formalin test, the hydroalcoholic extract (0.0001-0.1 mg/kg orally) also caused significant inhibition of both the early (neurogenic pain) and the late (inflammatory pain) phases of formalin-induced licking. However, it was more potent and efficacious in relation to the late phase of the formalin test. The capsaicin-induced nociception was also reduced at a dose of only 1.0 mg/kg orally. The hydroalcoholic extract significantly reduced the cinnamaldehyde-induced nociception at doses of 0.01, 0.1 and 1.0 mg/kg orally. Moreover, the hydroalcoholic extract (0.001-1.0 mg/kg orally) caused significant and dose-dependent inhibition of glutamate-induced pain. However, only rutin, but not phebalosin or aurapten, isolated from P. paniculata , administered intraperitoneally to mice, produced dose-related inhibition of glutamate-induced pain. Furthermore, the hydroalcoholic extract (0.1-100 mg/kg orally) had no effect in the tail-flick test. On the other hand, the hydroalcoholic extract caused a significant increase in the latency to response at a dose of 10 mg/kg orally, in the hot-plate test. The hydroalcoholic extract (0.1 mg/kg orally) antinociception, in the glutamate test, was neither affected by intraperitoenal treatment of animals with l-arginine (precursor of nitric oxide, 600 mg/kg) and naloxone (opioid receptor antagonist, 1 mg/kg.) nor associated with non-specific effects such as muscle relaxation or sedation. In addition, oral administration of hydroalcoholic extract produced a great inhibition of the pain-related behaviours induced by intrathecal injection of glutamate, N-methyl-d-aspartate (NMDA), IL-1 β and TNF-α , but not by α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA), kainate or trans-1-amino-1.3-cyclopentanediocarboxylic acid (trans-ACPD). Together, our results suggest that inhibition of glutamatergic ionotropic receptors, may account for the antinociceptive action reported for the hydroalcoholic extract from P. paniculata in models of chemical pain used in this study.

Beneficial effects of the ethanol extract of Caesalpinia pyramidalis on the inflammatory response and abdominal hyperalgesia in rats with acute pancreatitis

“…For instance, rutin decreased paw edema induced by carrageenan and arthritis induced by adjuvant plus carrageenan in rats (Guardia et al, 2001;Rotelli et al, 2003). It also reduced nociception induced by glutamate in mice paws (Lapa et al, 2009), renal inflammation induced by cysplatin in rats (Arjumand et al, 2011) and cystitis induced by cyclophosphamide in mice (Boeira et al, 2011). These studies reinforce the possibility of rutin's role in the effects observed in CBDO-induced pancreatitis in rats.…”

Antinociceptive activity of aqueous extract and isolated compounds of Lithrea molleoides

“…Both compounds were active in chemical nociceptive models used in this work, but with less activity than the whole extract. The phytochemical analysis showed that other compounds are present in the aqueous extract and they could contribute to the analgesic activity of the extract, since the antinociceptive activity of chlorogenic acid, caffeic acid, gallic acid, isoquercetin and rutin has previously reported (De Campos Buzzi et al, 2009;Dos Santos et al, 2006;Gorzalczany et al, 2011b;Krogh et al, 2000;Lapa et al, 2009). In accordance with our results, Leal et al (2011) showed antinociceptive properties induced by vanillic acid in the formalin test and compounds with similar chemical structure, such as vanillin also possess an antinociceptive profile (Park et al, 2009).…”

Antioxidant capacity and phenolic content of commonly used anti-inflammatory medicinal plants in Colombia

“…Aerial part Asthma Treatment Antidepressant (Bettio et al, 2011), antifungal (Johann et al, 2011), antinociceptive (Lapa et al 2009), antioxidant (Silva et al 2005), antiprotozoal , gastroprotective (Lapa et al 2007), vasorelaxant and hypotensive (Lapa et al 2011 Anticancer (Adebayo et al, 2010), antioxidant (Adebayo et al, 2010), antiprotozoal (Nour et al, 2010), analgesic, antipyretic and anti-inflammatory, anticoagulation, antimicrobial, anticonvulsant, chronic pain in osteoarthrotic patients, and neuromuscular blocking (Okunade, 2002). JCJ85…”