Antinociceptive effect of Lonchocarpus araripensis lectin: activation of l-arginine/NO/cGMP/K+ATP signaling pathway

Assreuy, Ana Maria Sampaio; Amorim, Renata Morais Ferreira; Martins, Stephanie Lian; Martins, Maria Gleiciane de Queiroz; Cajazeiras, João Batista; Silva, Mayara Torquato Lima; Pires, Alana F.; Nascimento, Kyria S.; Cavada, Benildo Sousa; Mota, Mário Rogério Lima

doi:10.1007/s10787-020-00729-z

Cited by 5 publications

(5 citation statements)

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“…It is also possible that the lectin is modulating the expression of in ammatory pathways as COX and TNF-α via the nuclear factor kappa B (NF-kB). Other Dalbergieae lectin also show modulator effect on the gene expression of in ammatory pathways such as Lonchocarpus araripensis [42].…”

Section: Discussionmentioning

confidence: 99%

The anti-inflamatory effect of Andira anthelmia lectin in rats involves inhibition of the prostanoid pathway, TNF-α and lectin domain

et al. 2022

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Objective: To investigate the effect and mechanisms of Andira anthelmia lectin in rat models of acute in ammation.Material: AAL anti-in ammatory activity was evaluated in Wistar rat models of paw edema, peritonitis and hyperalgesia.Methods: AAL (0.01-1 mg/kg i.v.) was injected 30 min before stimulation with carrageenan and with initial and late phase in ammatory mediators into the animals paw and peritoneum for evaluation of: cell migration (optical and intravital microscopy), paw edema (plethysmometry and histopathology); hyperalgesia (analgesimetry).Results: AAL inhibited leukocyte migration induced by carrageenan, mainly neutrophils to the peritoneal uid, decreasing leukocyte adhesion. In the peritoneal uid, AAL reduced the gene expression of TNF-α and cyclooxygenase, as well the levels of PGE 2 . ALL also inhibited the paw edema induced by carrageenan, mainly the late phase, but also the edema induced by serotonin, histamine, TNF-α, PLA 2 and PGE 2 , but not by L-arginine and bradykinin. In this model, AAL inhibited mechanical hypernociception induced by TNF-α, PGE 2 , db-cAMP and capsaicin, and the activity of myeloperoxidase in the paw tissues.Conclusion: AAL presents anti-in ammatory effect in acute models of rat in ammation involving the participation of prostaglandins, TNF-α and lectin domain.

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Section: Discussionmentioning

confidence: 99%

The anti-inflamatory effect of Andira anthelmia lectin in rats involves inhibition of the prostanoid pathway, TNF-α and lectin domain

et al. 2022

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“…CB1 agonists are known to inhibit membrane excitation and Ca 2+ conductance and also to increase potassium conductance, inducing an antinociceptive effect [21], in addition to stimulate nitric oxide synthesis [22]. In this line, the LAL antinociceptive effect involves the activation of the L-arginine/NO/GMPc/K+ATP pathway [6] and the decrease in the total Na+ current [5]. Moreover, CB2 agonists play an important role in the control of the inflammatory process, especially in the initial events that modulate the communication between endothelial cells and leukocytes, leading to inhibition of cell migration [23].…”

Section: Discussionmentioning

confidence: 99%

“…The lectin isolated from Lonchocarpus araripensis (LAL), belonging to Dalbergieae tribe, recognizes and specifically binds to N-acetyl-D-glucosamin [2]. Several effects have already been evidenced for LAL, such as vasorelaxant [3], anti-inflammatory [2,4], and antinociceptive [5,6], also a protective effect on sepsis model [7]. In respect to the antinociceptive activity, LAL has a pleiotropic effect in several nociceptive pathways, being associated to the modulation of inflammatory and/or hypernociceptive mediators [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…Several effects have already been evidenced for LAL, such as vasorelaxant [3], anti-inflammatory [2,4], and antinociceptive [5,6], also a protective effect on sepsis model [7]. In respect to the antinociceptive activity, LAL has a pleiotropic effect in several nociceptive pathways, being associated to the modulation of inflammatory and/or hypernociceptive mediators [5,6]. However, there are no studies regarding LAL antinociception in the cannabinoid system.…”

Section: Introductionmentioning

confidence: 99%

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The Antinociceptive Effect of Lonchocarpus Araripensis Lectin is Mediated by Endocannabinoid Receptors

Amorim¹,

Oliveira²,

Cavada³

et al. 2021

International Journal of Scientific Advances

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The involvement of endocannabinoid receptors in the antinociceptive activity of Lonchocarpus araripensis lectin (LAL) was investigated in the model of carragenan-induced hypernociception. Swiss mice received LAL (10 mg/kg) by intravenous (i.v.) route 30 min before subcutaneous (s.c.) injection of carrageenan in the paws. Animals were treated with the antagonists of CB1 (AM251) or CB2 (AM630) cannabinoid receptors 30 min before the lectin. LAL inhibited the hypernociception induced by carrageenan (1 h: 11.87 ± 0.61 g; 3 h: 9.7 ± 0.70 g). AM251 (1 h: 4.75 ± 0.41 g; 3 h: 4.5 ± 0.53 g) and AM630 (1 h: 5.67 ± 0.56 g; 3 h: 5 ± 0.68 g) reversed the inhibitory effect of LAL. The antinociceptive effect of Lonchocarpus araripensis lectin is mediated by endocannabinoid receptors.

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“…Lipofundin MCT/LCT is involved in attenuating K ATP channel-induced vasodilation by inhibiting basally released endothelial NO and/or cGMP ( Lee et al, 2020 ). The antinociceptive effects of methanol extracts of B. spectabilis, cardamonin and Lonchocarpus araripensis lectin ether are partially or completely mediated by the NO/cGMP/K ATP pathways ( Assreuy et al, 2020 ; Ferdous et al, 2020 ; Pui Ping et al, 2020 ). The K ATP channel, a gastroprotective factor, is involved in the gastroprotective effects of N-acylarylhydrazone derivatives on ethanol-induced gastric lesions in mice via the NO/cGMP pathway ( da Silva Monteiro et al, 2019 ).…”

Section: Regulation Of K Atp Channels By Small Act...mentioning

confidence: 99%

Functional Regulation of KATP Channels and Mutant Insight Into Clinical Therapeutic Strategies in Cardiovascular Diseases

et al. 2022

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ATP-sensitive potassium channels (KATP channels) play pivotal roles in excitable cells and link cellular metabolism with membrane excitability. The action potential converts electricity into dynamics by ion channel-mediated ion exchange to generate systole, involved in every heartbeat. Activation of the KATP channel repolarizes the membrane potential and decreases early afterdepolarization (EAD)-mediated arrhythmias. KATP channels in cardiomyocytes have less function under physiological conditions but they open during severe and prolonged anoxia due to a reduced ATP/ADP ratio, lessening cellular excitability and thus preventing action potential generation and cell contraction. Small active molecules activate and enhance the opening of the KATP channel, which induces the repolarization of the membrane and decreases the occurrence of malignant arrhythmia. Accumulated evidence indicates that mutation of KATP channels deteriorates the regulatory roles in mutation-related diseases. However, patients with mutations in KATP channels still have no efficient treatment. Hence, in this study, we describe the role of KATP channels and subunits in angiocardiopathy, summarize the mutations of the KATP channels and the functional regulation of small active molecules in KATP channels, elucidate the potential mechanisms of mutant KATP channels and provide insight into clinical therapeutic strategies.

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Antinociceptive effect of Lonchocarpus araripensis lectin: activation of l-arginine/NO/cGMP/K+ATP signaling pathway

Cited by 5 publications

References 25 publications

The anti-inflamatory effect of Andira anthelmia lectin in rats involves inhibition of the prostanoid pathway, TNF-α and lectin domain

The anti-inflamatory effect of Andira anthelmia lectin in rats involves inhibition of the prostanoid pathway, TNF-α and lectin domain

The Antinociceptive Effect of Lonchocarpus Araripensis Lectin is Mediated by Endocannabinoid Receptors

Functional Regulation of KATP Channels and Mutant Insight Into Clinical Therapeutic Strategies in Cardiovascular Diseases

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