Antinociceptive and Anti-inflammatory Effects of the Hydroalcoholic Fraction from Leaves of Cenostigma macrophyllum Tul. var. acuminata Teles Freire (Leguminosae) in Rodents

Abstract

Introduction: Cenostigma macrophyllum Tul. var. acuminata Teles Freire (Leguminosae) is locally known as "caneleiro" and its barks, leaves and flowers are used in folk medicine against stomach disorders and abdominal pain. HPLC analysis of hydroalcoholic fraction from leaves (Cm-HAF) indicated two biflavones as main constituents: agathisflavone and amentoflavone.Purpose: To investigate antinociceptive and anti-inflammatory/antiarthritic effects of Cm-HAF in animal models. Materials and Methods:We used male Swiss mice (25-30 g) and female Wistar rats (150-210 g). Acute antinociception was evaluated using formalin, capsaicin and glutamate tests. Complete Freund´s adjuvant (CFA)-induced monoathritic rats were treated with Cm-HAF (50, 100 and 200 mg/kg p.o.) for 15 days and evaluated for acute D (0-1), sub-acute D (3-7) and chronic D (10-14) anti-inflammatory (paw edema) and antinociceptive effects (mechanical nociception). Histological evaluation of paws, blood urea nitrogen (BUN), creatinine and alanine-amino transferase (ALT) dosage, total body weight variation, wet weight of kidneys and liver and macroscopical evaluation of gastric mucosae were performed on D (14).Results: Cm-HAF produced significant antinociceptive effect in capsaicin, glutamate tests and in the late phase of formalin test. CFA-induced monoarthritic rats treated with Cm-HAF (50, 100 and 200 mg/kg p.o.) showed no significant differences on paw diameter or histological evaluation, but showed a significant antinociception (Cm-HAF 100 and 200 mg/kg p.o.) similar to indomethacin (IND -3 mg/kg p.o.). Conclusion:Cm-HAF has antinociceptive but not antiedematogenic/antiarthritic effects in rodents. These results contribute to the pharmacological knowledge of this species and support its ethnomedical use.

Application of response surface methodology for optimization of ultrasound‐assisted solid‐liquid extraction of phenolic compounds from Cenostigma macrophyllum

“…It is popularly known as “canela‐de‐velho,” “caneleiro,” and “catingueira.” 5 The leaves of this species are used in folk medicine for the treatment of stomach and intestinal diseases 5 . The extracts and compounds found in this plant show a wide range of biological activities, including antioxidant, 6 gastroprotective, 7 acetylcholinesterase inhibitory, 8 antinociceptive, and anti‐inflammatory effects 8–10 . Phytochemical investigation of the C .…”

“…5 The extracts and compounds found in this plant show a wide range of biological activities, including antioxidant, 6 gastroprotective, 7 acetylcholinesterase inhibitory, 8 antinociceptive, and anti-inflammatory effects. [8][9][10] Phytochemical investigation of the C. macrophyllum leaves led to the isolation and identification of flavonoids, bioflavonoids, and benzoic acid derivatives as the main polyphenol compounds. 11,12 An analytical method has been developed and validated for the simultaneous determination and quantification of bioflavonoids (agathisflavone and amentoflavone) and gallic acid and its derivatives in the C. macrophyllum leaves.…”

Antinociceptive activity of theCaesalpinia eriostachysBenth. ethanolic extract, fractions, and isolated compounds in mice

“…Var. acuminate Teles Freire that contained agathisflavone showed antinociceptive action in rodents (Cavalcanti et al, 2017), neuroprotective and anti‐inflammatory effects (Dos Santos Souza et al., 2018), and decrease in oxidative stress in the brain (Dumitru et al., 2019). However, so far, there is no available research on the antinociceptive activity of agathisflavone as an isolated compound in various pain models.…”

Evaluation of anti-inflammatory, antinociceptive and biological activities of Cenostigma macrophyllum standardized extracts and determination and quantification of the main metabolites

“…In other studies dealing with antinociceptive actions of the ethanol, ethyl acetate extract and emulsion of hexane extract obtained from stem bark in streptozotocin (STZ)-induced diabetic rats showed a pain threshold higher than those in the STZ-vehicle group indicating antinociceptive activity but in this study the chemical composition of the extracts were presumed based on simple thin layer chromatography analysis and no evidences about the phenolics content. [8][9][10] Phytochemical studies with C. macrophyllum have led to the isolation and identication of avonoids, biavonoids, benzoic acid derivatives, peptide derivatives, triterpenoids, steroids, vitamin A and E, fatty acids, coumarin and volatile esters. 3,11,12 However, there are no reports in the literature on the development of a validated chromatographic method for the quantication of active principles present in this species.…”

“…In other studies dealing with antinociceptive actions of the ethanol, ethyl acetate extract and emulsion of hexane extract obtained from stem bark in streptozotocin (STZ)-induced diabetic rats showed a pain threshold higher than those in the STZ-vehicle group indicating antinociceptive activity but in this study the chemical composition of the extracts were presumed based on simple thin layer chromatography analysis and no evidences about the phenolics content. 8–10 …”