Antinociceptive and anti-inflammatory activities of ethyl acetate fraction from Zanthoxylum armatum in mice

Guo, Tao; Deng, Yunxia; Xie, Hui; Chen, Yao; Cai, Cheng-Cheng; Pan, Shengli; Wang, Yanglin

doi:10.1016/j.fitote.2010.11.004

Cited by 69 publications

(41 citation statements)

References 14 publications

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“…3c) as compared to LPS treatment alone. anti-vascular inflammatory, antiproliferative, antinociceptive and anticaries potentials (Guo et al, 2010;Li et al, 2010;Pereira et al, 2010;Chou et al, 2011;Rodriguez-Guzman et al, 2011). Unfortunately, the scientific evidence of LPFE (Z. acanthopodium DC.)…”

Section: Effects Of Lpfe On the Protein And Gene Expression Of Inflammentioning

confidence: 99%

“…Evidences showed that the methanol extracts of Z. chalybeum and Z. usambarense, medicinal plants from Kenya, possessed dual anti-bacterial and anti-inflammatory activities (Matu and Staden, 2003). Also, the ethyl acetate fraction of Z. armatum strongly inhibited xyleneinduced ear swelling in mice in vivo (Guo et al, 2010). Some active constituents isolated from Zanthoxylum species, i.e., glycoprotein, phenylpropenoids, neolignan and alkaloids, were found to have anti-inflammatory properties through blocking the expression of inflammatory cytokines (TNF-α, IL-1β and IL-6) in various inflammatory cells in vitro (Chen et al, 2007;2008;Hu et al, 2006;Lee and Lim, 2009a;2009b).…”

Section: Effects Of Lpfe On the Protein And Gene Expression Of Inflammentioning

confidence: 99%

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Lemon Pepper Fruit Extract (Zanthoxylum acanthopodium DC.) Suppresses the Expression of Inflammatory Mediators in Lipopolysaccharide-Induced Macrophages In Vitro

Yanti¹,

Pramudito²,

Nuriasari³

et al. 2011

American Journal of Biochemistry and Biotechnology

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Problem statement: Lemon pepper fruits (Zanthoxylum acanthopodium DC.; Rutaceae) have been used as a traditional source against stomach ache by Batak people in North Sumatera province, Indonesia. However, its scientific evidence for treatment of inflammatory disorders particularly gastritis has not been reported. Approach: Here, we investigated the inhibitory effects of Lemon Pepper Fruit Extract (LPFE) against inflammatory biomarkers by conducting cell culture experiments in vitro. The fruits of lemon pepper were dried and extracted twice in 70% ethanol, followed by evaporation and freeze-drying. The concentrated extract was further tested for its potential inhibition on the protein and gene expression of several inflammatory biomarkers, i.e. ) and LPS (2 µg mL −1 ) had no cytotoxicity effects on macrophages. LPFE dose dependently decreased the expression of TNF-α and COX-2 proteins and MMP-9 activity in macrophages treated with LPS. At the gene level, LPFE were effectively found to block the mRNA expression of TNF-α, IL-6, iNOS, COX-2 and MMP-9. Conclusion: Our results suggest that LPFE significantly inhibits selected inflammatory biomarkers at the protein and gene levels in LPS-induced macrophages. Further in vivo study using animal models is needed to determine the exact anti-inflammatory potential of LPFE.

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Section: Effects Of Lpfe On the Protein And Gene Expression Of Inflammentioning

confidence: 99%

Section: Effects Of Lpfe On the Protein And Gene Expression Of Inflammentioning

confidence: 99%

Lemon Pepper Fruit Extract (Zanthoxylum acanthopodium DC.) Suppresses the Expression of Inflammatory Mediators in Lipopolysaccharide-Induced Macrophages In Vitro

Yanti¹,

Pramudito²,

Nuriasari³

et al. 2011

American Journal of Biochemistry and Biotechnology

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“…Pepper is commonly used in traditional Chinese medicine and is among the eight main condiments in China. Current research shows that Zanthoxylum alkylamides mainly comprise α-, β-and γ alkylamides, which have antioxidant (Batool et al 2010, Xia et al 2011, anticancer (Chou et al 2011) and hepatoprotective (Guo et al 2011) effects.…”

Section: Introductionmentioning

confidence: 99%

Zanthoxylum alkylamides ameliorate protein metabolism disorder in STZ-induced diabetic rats

Ren

Zhu

Xia

et al. 2017

Journal of Molecular Endocrinology

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This study aimed to evaluate the protein metabolism effect of Zanthoxylum alkylamides and to explore the potential mechanism in streptozotocin (STZ)-induced diabetic rats. Diabetic rats were orally treated with 2, 4 and 8 mg per kg bw of alkylamides daily for 28 days. Alkylamides decreased the relative weight of the liver and food intake, significantly increased the relative skeletal muscle weight and significantly decreased the blood urea nitrogen levels. Insulin, insulin-like growth factor 1, total protein (TP) and albumin (ALB), globular proteins and ALB proteins/globulin protein levels in serum significantly increased. TP, RNA content and RNA/DNA ratio significantly increased in the skeletal muscle of diabetic rats. Real-time quantitative polymerase chain reaction results indicated that alkylamides significantly increased the mRNA expression of insulin receptor (InR), IGF1 and insulin-like growth factor 1 receptor (IGF1R) in the liver and skeletal muscle. Moreover, the mRNA and protein expression levels of PI3K, PKB and mTOR significantly increased, whereas those of atrogin-1, muscle ring finger 1 and FOXO in the skeletal muscle significantly decreased. Alkylamides may advance protein synthesis by the PI3K/PKB/mTOR signalling pathway and attenuate the catabolism of protein through the ubiquitin-proteasome pathway. Therefore, it was possible that alkylamides ameliorate protein metabolism disorders in diabetic rats by activating the mTOR pathway.

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“…Scientific bioactivity determination studies have revealed its larvicidal (Tiwary et al, 2007), hepatoprotective, antioxidant (Ranawat et al, 2010), antinociceptive, antiinflammatory, and antipyretic activities (Guo et al, 2011). Various phytopharmaceuticals, such as berberine, dictamnine, xanthoplanine, armatamid, asarinin, fargesin, a-and b-amyrins and lupeol are present in the plant (Kalia et al, 1999;Nadkarni, 2002).…”

Section: Introductionmentioning

confidence: 99%

Regulation of apoptosis through bcl-2/bax proteins expression and DNA damage byZanthoxylum alatum

Karmakar

Haldar

Chakraborty

et al. 2015

Pharmaceutical Biology

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Context: Many of the major chemotherapeutic agents are secondary metabolites found in nature. Zanthoxylum alatum Roxb. (Rutaceae) is traditionally used in the treatment of various diseases. Objective: The present study evaluates the apoptotic activity of methanol extract of Z. alatum (MEZA) on Ehrlich ascites tumor (EAT) in Swiss albino mice. Materials and methods: The presence of flavonoids in MEZA was standardized by HPLC. The in vitro cytotoxicity of MEZA was measured by the MTT assay. The in vivo antitumor activity of MEZA (100 and 200 mg/kg b.w., i.p. for 9 days) was also evaluated. On the 10th day, EAT tumor volume, cell viability, and hematological parameters were assayed. Apoptotic morphology was determined by acredine orange/ethedium bromide using fluorescence microscopy. Apoptosis percentage was measured by flow cytometric analysis using annexine-V-FITC. Also, DNA damage and bcl-2/bax were estimated by UV-method and western blot, respectively. Results and discussion: HPLC analysis revealed presence of three flavonoids, rutin, myricetin, and quercetin. MEZA showed satisfactory cytotoxicity in MTT assay (IC 50 ¼ 111.50 mg/ml). The extract significantly (p50.01) changed the tumor volume, viable, non-viable cell count, and hematological parameters towards the normal. Apoptotic activity of MEZA was confirmed by acridine orange/ethidium bromide staining, annexin-V-FITC staining, DNA fragmentation, and Bcl-2/Bax ratio. Conclusion: The study showed that MEZA has antitumor activity which may be due to the presence of flavonoids in the extract.

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Antinociceptive and anti-inflammatory activities of ethyl acetate fraction from Zanthoxylum armatum in mice

Cited by 69 publications

References 14 publications

Lemon Pepper Fruit Extract (<i>Zanthoxylum acanthopodium</i> DC.) Suppresses the Expression of Inflammatory Mediators in Lipopolysaccharide-Induced Macrophages <i>In Vitro</i>

Lemon Pepper Fruit Extract (<i>Zanthoxylum acanthopodium</i> DC.) Suppresses the Expression of Inflammatory Mediators in Lipopolysaccharide-Induced Macrophages <i>In Vitro</i>

Zanthoxylum alkylamides ameliorate protein metabolism disorder in STZ-induced diabetic rats

Regulation of apoptosis through bcl-2/bax proteins expression and DNA damage byZanthoxylum alatum

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