Antinociceptive and anti-hypernociceptive effects of Se-phenyl thiazolidine-4-carboselenoate in mice

Abstract

In this study, the antinociceptive, anti-hypernociceptive and toxic effects of orally administered (R)-Se-phenyl thiazolidine-4-carboselenoate (Se-PTC, 1-50 mg/kg) were evaluated in mice. Se-PTC did not change plasma aspartate (AST) and alanine aminotransferase (ALT) activities or urea and creatinine levels. Furthermore, in an open field test, Se-PTC did not alter the number of crossings and rearing. Se-PTC significantly reduced the amount of writhing when assessed by acetic acid-induced visceral nociception and attenuated the licking time of the injected paw in the early and late phases of a formalin test. In addition, Se-PTC reduced nociception produced by intra-plantar (i.pl.) injection of glutamate, capsaicin, cinnalmaldehyde, bradykinin, phorbol myristate acetate and 8-Bromo-cAMP. Se-PTC caused a significant increase in hot plate and tail-immersion response latencies, but the antinociceptive effect of Se-PTC in the tail immersion was not abolished by pretreatment with the non-selective opioid receptor antagonist, naloxone. Se-PTC (25 mg/kg) significantly inhibited nociceptive behavior induced by intrathecal (i.t.) injection of glutamate, N-methyl-D-aspartate (NMDA) and (±)-1-aminocyclopentane-trans-1,3-dicarboxylic acid (trans-ACPD), but failed to affect nociception induced by kainate and α-amino-3-hydroxy-5-mehtyl-4-isoxazolepropionic acid (AMPA). Mechanical hypernociception induced by carrageenan and Complete Freund's Adjuvant was attenuated by Se-PTC administration. These results indicate that Se-PTC produces antinociception in several models of nociception.

Antinociceptive and anti-hyperalgesic effects of bis(4-methylbenzoyl) diselenide in mice: Evidence for the mechanism of action

“…Our results provide evidence that BMD inhibited PMA and 8-bromocAMP-induced nociception in mice. Thus, these results are similar to those shown in others study of our group (Jesse et al, 2009b,c;Pavin et al, 2011;Pinto et al, 2008;Savegnago et al, 2007a;Wilhelm et al, 2009).…”

“…Pretreatment of animals with naloxone (a non-selective opioid receptor antagonist) did not reverse the antinociceptive effect caused by BMD, demonstrating that the opioid system is not involved in this effect. In accordance with these results, we did not observe any opioid system involvement in the antinociceptive effect of other organoselenium compounds (Luchese et al, 2010;Pavin et al, 2011;Pinto et al, 2008;Savegnago et al, 2007a).…”

“…Furthermore, dipyrone, indomethacin and acetaminophen produced antinociceptive effects on the second phase of the formalin test (Abbott & Hellemans, 2000). Additionally, morphine (Shibata et al, 1989), organoselenium compounds (Luchese et al, 2010;Pavin et al, 2011;Savegnago et al, 2007a) and other nonsteroidal anti-inflammatory drugs (NSAIDs) (Martindale et al, 2001) were reported to inhibit nociception in both phases of the formalin test.…”

“…Intraplantar injection of glutamate into the mouse hindpaw produces nociceptive-like behaviors of rapid onset and short duration (about 15 min). As well as, other organoselenium compounds (Jesse et al, 2007Pavin et al, 2011;Pinto et al, 2008;Savegnago et al, 2007a,c;Wilhelm et al, 2009), the BMD reduced the nociceptive response induced by glutamate in mice. Of note, aspirin and acetaminophen demonstrated an antinociceptive effect in the glutamate test (Choi et al, 2001).…”

“…Recently, our group of research showed hepatoprotective effect of bis(4-methylbenzoyl) diselenide (BMD), an disubstituted diaryl diselenide, against carbon tetrachloride (CCl 4 )-induced oxidative damage in mice possibly by modulating the antioxidant status (Filho et al, 2013). Moreover, in previous publications, the antinociceptive and anti-inflammatory properties of organoselenium compounds were reported (Jesse et al, 2007(Jesse et al, , 2009aLuchese et al, 2010;Savegnago et al, 2006Savegnago et al, , 2007aPinto et al, 2008;Wilhelm et al, 2009;Pavin et al, 2011) which are potentially useful drugs for pain management.…”