Antinociceptive activity of alcoholic extract of Hemidesmus indicus R.Br. in mice

Verma, Prashant; Joharapurkar, Amit; Chatpalliwar, Vivekanand A.; Asnani, Alpana J.

doi:10.1016/j.jep.2005.05.039

Cited by 85 publications

(45 citation statements)

References 15 publications

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“…An ethanolic extract was prepared [17] using a soxlet apparatus, and screened for its hepatoprotective property. The ethanolic extract (yield: 9.2%) was dark brown in color and sticky in consistency, and gave positive results for flavonoids, terpenoids, tannins, coumarins and glycoside, and negative results for alkaloids, anthraquinones, lactones/esters, proteins/amino acids and saponins [18].…”

Section: Hemidesmus Indicusmentioning

confidence: 99%

Hemidesmus indicus Protects against ethanol-induced liver toxicity

Saravanan

Nalini

2008

Cellular and Molecular Biology Letters

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Alcoholic liver disease (ALD) is one of the most common diseases in modern society. A large number of studies are in progress aiming to identify natural substances that would be effective in reducing the severity of ALD. Although there are currently a number of drugs on the market, their long-term use can have numerous side effects. Hemidesmus indicus is an indigenous Ayurvedic medicinal plant used in soft drinks in India. In this study, we examined the effects of its ethanolic root extract on experimental liver damage in order to evaluate its hepatoprotective effects against hepatotoxicity induced in rats by ethanol at a dosage of 5 g/kg body weight for 60 days. The H. indicus root extract was given at a dose of 500 mg/kg body weight for the last 30 days of the experiment. The animals were monitored for food intake and weight gain. The liver was analysed for the degree of lipid peroxidation using thiobarbituric acid reactive substances (TBARS) and antioxidant status using the activities of glutathione-depedendant enzymes. The degree of liver damage was analysed using serum marker enzyme activities, the total protein, albumin, globulin, ceruloplasmin and liver glycogen contents, and the A/G ratio. The Fourier transform infrared spectra (FT-IR) of the liver tissues were recorded in the region of 4000-400 cm -1 . The ethanol-fed rats showed significantly elevated liver marker enzyme activities, lipid peroxidation levels and reduced antioxidant levels as compared to the control rats. Oral administration of H. indicus for the latter 30 days resulted in an increased food intake and weight gain, decreased TBARS levels, near normal levels of glutathione-dependent enzymes, increased total protein, albumin, globulin and liver glycogen contents, an increased A/G ratio, and decreased liver marker enzyme activities and ceruloplasmin levels. The relative intensity of the liver FT-IR bands for the experimental groups were found to be altered significantly (p < 0.05) compared to the control samples. For the group that had H. indicus co-administered with ethanol, the intensity of the bands was near normal. Moreover, the results of the FT-IR study correlated with our biochemical results.

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Section: Hemidesmus Indicusmentioning

confidence: 99%

Hemidesmus indicus Protects against ethanol-induced liver toxicity

Saravanan

Nalini

2008

Cellular and Molecular Biology Letters

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“…Inhibition of the chronic phase of the formalin test by the extract may be due to the inflammations, which cause chemicals such as prostaglandins F2α and E2 to be released. In some cases certain amounts of these chemicals can sensitize central nociceptive neurons (28).…”

Section: Discussionmentioning

confidence: 99%

In Vivo Antinociceptive Effects of Persian Shallot (Allium hirtifolium) in Male Rat

Mohammadi¹,

Mahmoodi²,

Zarei³

et al. 2015

Avicenna J Neuro Psych Physio

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Background:The tendency toward using herbal plants instead of synthetic drugs is increasing in recent years because of their lower adverse effects and high varieties of efficient components. Objectives: In this investigation, analgesic effects of hydroalcoholic leaf extract of Allium hirtifolium were studied in male rats. Materials and Methods: A total of 48 adult male rats were divided eight groups: control, intraperitoneal 50, 100, and 200 mg/kg of extract, 200 mg/kg of A. hirtifolium extract plus aspirin, aspirin (1 mg/kg), morphine (1 mg/kg), and 200 mg/kg of A. hirtifolium extract plus naloxone (1 mg/kg). The analgesic effects of A. hirtifolium were assessed with writhing, tail-flick, and formalin tests. The data were compared with control by one-way ANOVA and Tukey post hoc test. Results: Allium hirtifolium extract at (200 mg/kg dosage), alone and in combination with aspirin, had shown antinociceptive effect in writhing, tail-flick, and formalin tests (P < 0.01). Allium hirtifolium extract (at 100 mg/kg dosage) had just shown analgesic effects on tailflick and formalin (chronic phase) tests. Conclusions: It looks that A. hirtifolium has antinociceptive effects that might be due to flavonoids and saponins in plant the analgesic effect of which was demonstrated previously.

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“…The test consists of two different phases: The first one (neurogenic phase) is generated in the periphery through the activation of nociceptive neurons by the direct action of formalin and the second phase (inflammatory phase) occurs through the activation of the ventral horn neurons at the spinal cord. The late phase is due to inflammation causing a release of serotonin, histamine, bradykinin and prostaglandins, which at least to some degree can cause the sensitization of the central nociceptive neurons (Verma et al, 2005). Morphine, a typical narcotic analgesic drug, can inhibit nociception in both phases (Shibata et al, 1989) while NSAIDs, by acting supraspinally, can also reduce the pain in both phases (Martindale et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Analgesic and Anti-inflammatory Activity of <i>Euphorbia antiquorum</i> Linn

Das

Alam

Bhattacharjee

et al. 2015

American Journal of Pharmacology and Toxicology

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Euphorbia antiquorum (L.) is used as traditional medicine for various ailments in Bangladesh. But the scientific basis for its use especially in pain and inflammation remains largely unknown. Therefore, the present study was designed to evaluate analgesic and anti-inflammatory effect of the aqueous ethanolic extract of the whole plant. The analgesic activity was evaluated by hot plate, acetic acid induced writhing and formalin induced writhing methods in Swiss Albino mice at the doses of 250 and 500 mg kg −1 body weight. The extract was also investigated for the anti-inflammatory effect on Long Evans rats at above mentioned doses using carrageenan induced rat paw edema method. Phytochemical analysis of the extract revealed the presence of tannins, alkaloids, flavonoids, saponins and terpenoids. The extract elicited a significant (p<0.01) analgesic activity in a dose dependent fashion in both the early and late phases of formalin test and also in hot plate and acetic acid induced writhing tests at both the doses employed. In acetic acid induced writhing test, the extract (at 500 mg kg −1 ) produced a maximum inhibition of writhing reaction by 51.13% (p<0.001), which is comparable to that of the standard drug Diclofenac sodium (58.80%). The extract also significantly inhibited the licking response at the dose of 500 mg kg −1 in both the early phase (63.64%, p<0.01) and the late phase (66.43%, p<0.01) of formalin test while the standard drug inhibited by 64.20% and 72.03%, respectively. The oral administration of the extract significantly (p<0.001) inhibited inflammatory response induced by carrageenan in a dose dependent manner. The most prominent inhibition of 74.25% (250 mg kg −1 ) and 78.44% (500 mg kg −1 ) were observed at the 4th hour of study. Presence of various chemical constituents in the extract of Euphorbia antiquorum especially tannins, flavanoids, terpenoids and alkaloids might be responsible for the observed analgesic and anti-inflammatory effects. These experimental findings lend pharmacological support to the suggested folkloric uses of the plant in the management and/or control of pain as well as inflammatory conditions.

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Antinociceptive activity of alcoholic extract of Hemidesmus indicus R.Br. in mice

Cited by 85 publications

References 15 publications

Hemidesmus indicus Protects against ethanol-induced liver toxicity

Hemidesmus indicus Protects against ethanol-induced liver toxicity

In Vivo Antinociceptive Effects of Persian Shallot (Allium hirtifolium) in Male Rat

Analgesic and Anti-inflammatory Activity of <i>Euphorbia antiquorum</i> Linn

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